A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS)
Background Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which...
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ftmit:oai:dspace.mit.edu:1721.1/70111 2023-06-11T04:10:14+02:00 A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) Lovoll, Marie Wiik-Nielsen, Jannicke Grove, Soren Wiik-Nielsen, Christer R. Kristoffersen, Anja B. Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S. Nederbragt, Alexander J. Meyerson, Matthew L. Rimstad, Espen Tengs, Torstein Harvard University--MIT Division of Health Sciences and Technology Meyerson, Matthew L. 2010-07 application/pdf http://hdl.handle.net/1721.1/70111 en_US eng BioMed Central Ltd. http://dx.doi.org/10.1186/1743-422x-7-309 Virology Journal 1743-422X http://hdl.handle.net/1721.1/70111 Løvoll, Marie et al. “A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo Salar) with Cardiomyopathy Syndrome (CMS).” Virology Journal 7.1 (2010): 309. Web. Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 BioMed Central Article http://purl.org/eprint/type/JournalArticle 2010 ftmit https://doi.org/10.1186/1743-422x-7-309 2023-05-29T08:43:24Z Background Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which is associated with another cardiac disease in Atlantic salmon; heart and skeletal muscle inflammation (HSMI). In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the PRV genome were identified. In addition, a sequence contig from a novel totivirus could also be constructed. Using RT-qPCR, levels of PRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. In situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses, our results are compatible with a hypothesis where, in the experimental challenge studied, PRV behaves as an opportunist whereas the totivirus might be more directly linked with the development of CMS. Research Council of Norway (183196/S40) Research Council of Norway (1869072) Research Council of Norway (1869073) Article in Journal/Newspaper Atlantic salmon Salmo salar DSpace@MIT (Massachusetts Institute of Technology) Norway Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Virology Journal 7 1 309 |
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Open Polar |
collection |
DSpace@MIT (Massachusetts Institute of Technology) |
op_collection_id |
ftmit |
language |
English |
description |
Background Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which is associated with another cardiac disease in Atlantic salmon; heart and skeletal muscle inflammation (HSMI). In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the PRV genome were identified. In addition, a sequence contig from a novel totivirus could also be constructed. Using RT-qPCR, levels of PRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. In situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses, our results are compatible with a hypothesis where, in the experimental challenge studied, PRV behaves as an opportunist whereas the totivirus might be more directly linked with the development of CMS. Research Council of Norway (183196/S40) Research Council of Norway (1869072) Research Council of Norway (1869073) |
author2 |
Harvard University--MIT Division of Health Sciences and Technology Meyerson, Matthew L. |
format |
Article in Journal/Newspaper |
author |
Lovoll, Marie Wiik-Nielsen, Jannicke Grove, Soren Wiik-Nielsen, Christer R. Kristoffersen, Anja B. Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S. Nederbragt, Alexander J. Meyerson, Matthew L. Rimstad, Espen Tengs, Torstein |
spellingShingle |
Lovoll, Marie Wiik-Nielsen, Jannicke Grove, Soren Wiik-Nielsen, Christer R. Kristoffersen, Anja B. Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S. Nederbragt, Alexander J. Meyerson, Matthew L. Rimstad, Espen Tengs, Torstein A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) |
author_facet |
Lovoll, Marie Wiik-Nielsen, Jannicke Grove, Soren Wiik-Nielsen, Christer R. Kristoffersen, Anja B. Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S. Nederbragt, Alexander J. Meyerson, Matthew L. Rimstad, Espen Tengs, Torstein |
author_sort |
Lovoll, Marie |
title |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) |
title_short |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) |
title_full |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) |
title_fullStr |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) |
title_full_unstemmed |
A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS) |
title_sort |
novel totivirus and piscine reovirus (prv) in atlantic salmon (salmo salar) with cardiomyopathy syndrome (cms) |
publisher |
BioMed Central Ltd. |
publishDate |
2010 |
url |
http://hdl.handle.net/1721.1/70111 |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Norway Slaughter |
geographic_facet |
Norway Slaughter |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BioMed Central |
op_relation |
http://dx.doi.org/10.1186/1743-422x-7-309 Virology Journal 1743-422X http://hdl.handle.net/1721.1/70111 Løvoll, Marie et al. “A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo Salar) with Cardiomyopathy Syndrome (CMS).” Virology Journal 7.1 (2010): 309. Web. |
op_rights |
Creative Commons Attribution http://creativecommons.org/licenses/by/2.0 |
op_doi |
https://doi.org/10.1186/1743-422x-7-309 |
container_title |
Virology Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
309 |
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1768384530728615936 |