Characterizing cobalamin cycling by Antarctic marine microbes across multiple scales

• Main Author: Rao, Deepa,Ph.D.Massachusetts Institute of Technology.
• Other Authors: Michael J. Follows., Joint Program in Oceanography/Applied Ocean Science and Engineering., Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences., Woods Hole Oceanographic Institution., Joint Program in Oceanography/Applied Ocean Science and Engineering, Massachusetts Institute of Technology. Department of Earth, Atmospheric, and Planetary Sciences, Woods Hole Oceanographic Institution
• Format: Thesis
• Language: English
• Published: Massachusetts Institute of Technology 2020
• Subjects: Joint Program in Oceanography/Applied Ocean Science and Engineering; Earth; Atmospheric; and Planetary Sciences; Woods Hole Oceanographic Institution; Antarctic; Southern Ocean; Antarc*; Antarctica
• Online Access: https://hdl.handle.net/1721.1/127908

Thesis: Ph. D., Joint Program in Oceanography/Applied Ocean Science and Engineering (Massachusetts Institute of Technology, Department of Earth, Atmospheric, and Planetary Sciences; and the Woods Hole Oceanographic Institution), May, 2020 Cataloged from the official PDF of thesis. Includes bibliographical references (pages 161-183). Highly productive marine microbial communities in the coastal Southern Ocean sustain the broader Antarctic ecosystem and play a key role in Earth's climate via the biological pump. Regional phytoplankton growth is primarily limited by iron and co-limited by cobalamin (vitamin B₁₂), a trace cobalt-containing organometallic compound only synthesized by some bacteria and archaea. These micronutrients impact primary production and the microbial ecology of the two keystone phytoplankton types: diatoms and Phaeocystis antarctica. This thesis investigates microbe-driven cobalamin cycling in Antarctic seas across multiple spatiotemporal scales. I conducted laboratory culture experiments with complementary proteomics and transcriptomics to investigate the B₁₂-ecophysiology of P. antarctica strain CCMP 1871 morphotypes under iron-B₁₂ co-limitation. We observed colony formation under higher iron treatments, and a facultative use of B₁₂-dependent (MetH) and B₁₂-independent (MetE) methionine synthase isoforms in response to vitamin availability, demonstrating that this strain is not B₁₂-auxotrophic. Through comparative 'omics, we identified a putative MetE protein in P. antarctica abundant under low B₁₂, which is also found in other marine microbes. Across Antarctic seas, community-scale cobalt and B₁₂ uptake rates were measured by ⁵⁷Co radiotracer incubation experiments and integrated with hydrographic and phytoplankton pigment data. I observed significant correlations between uptake fluxes and environmental variables, providing evidence for predominantly diatom-driven uptake of these micronutrients in warmer, fresher surface waters with notable regional differences. To date, this work ...