Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations
Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pat...
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ftmit:oai:dspace.mit.edu:1721.1/117414 2023-06-11T04:11:09+02:00 Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations Bruto, Maxime Labreuche, Yannick James, Adèle Piel, Damien Chenivesse, Sabine Petton, Bruno Le Roux, Frédérique Polz, Martin F Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Parsons Laboratory for Environmental Science and Engineering (Massachusetts Institute of Technology) Polz, Martin F 2018-08-20T14:24:42Z application/pdf http://hdl.handle.net/1721.1/117414 unknown Nature Publishing Group http://dx.doi.org/10.1038/s41396-018-0245-3 ISME Journal 1751-7362 1751-7370 http://hdl.handle.net/1721.1/117414 Bruto, Maxime et al. “Ancestral Gene Acquisition as the Key to Virulence Potential in Environmental Vibrio Populations.” The ISME Journal (August 2018) © 2018 The Authors orcid:0000-0001-9296-3733 Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ Nature Article http://purl.org/eprint/type/JournalArticle 2018 ftmit https://doi.org/10.1038/s41396-018-0245-3 2023-05-29T08:44:05Z Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus clade, virulence represents an ancestral trait but has been lost from several populations. Two loci are necessary for virulence, the first being widely distributed across the Splendidus clade and consisting of an exported conserved protein (R5.7). The second is a MARTX toxin cluster, which only occurs within V. splendidus and is for the first time associated with virulence in marine invertebrates. Varying frequencies of both loci among populations indicate different selective pressures and alternative ecological roles, based on which we suggest strategies for epidemiological surveys. Article in Journal/Newspaper Crassostrea gigas DSpace@MIT (Massachusetts Institute of Technology) The ISME Journal 12 12 2954 2966 |
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DSpace@MIT (Massachusetts Institute of Technology) |
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description |
Diseases of marine animals caused by bacteria of the genus Vibrio are on the rise worldwide. Understanding the eco-evolutionary dynamics of these infectious agents is important for predicting and managing these diseases. Yet, compared to Vibrio infecting humans, knowledge of their role as animal pathogens is scarce. Here we ask how widespread is virulence among ecologically differentiated Vibrio populations, and what is the nature and frequency of virulence genes within these populations? We use a combination of population genomics and molecular genetics to assay hundreds of Vibrio strains for their virulence in the oyster Crassostrea gigas, a unique animal model that allows high-throughput infection assays. We show that within the diverse Splendidus clade, virulence represents an ancestral trait but has been lost from several populations. Two loci are necessary for virulence, the first being widely distributed across the Splendidus clade and consisting of an exported conserved protein (R5.7). The second is a MARTX toxin cluster, which only occurs within V. splendidus and is for the first time associated with virulence in marine invertebrates. Varying frequencies of both loci among populations indicate different selective pressures and alternative ecological roles, based on which we suggest strategies for epidemiological surveys. |
author2 |
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering Parsons Laboratory for Environmental Science and Engineering (Massachusetts Institute of Technology) Polz, Martin F |
format |
Article in Journal/Newspaper |
author |
Bruto, Maxime Labreuche, Yannick James, Adèle Piel, Damien Chenivesse, Sabine Petton, Bruno Le Roux, Frédérique Polz, Martin F |
spellingShingle |
Bruto, Maxime Labreuche, Yannick James, Adèle Piel, Damien Chenivesse, Sabine Petton, Bruno Le Roux, Frédérique Polz, Martin F Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
author_facet |
Bruto, Maxime Labreuche, Yannick James, Adèle Piel, Damien Chenivesse, Sabine Petton, Bruno Le Roux, Frédérique Polz, Martin F |
author_sort |
Bruto, Maxime |
title |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_short |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_full |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_fullStr |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_full_unstemmed |
Ancestral gene acquisition as the key to virulence potential in environmental Vibrio populations |
title_sort |
ancestral gene acquisition as the key to virulence potential in environmental vibrio populations |
publisher |
Nature Publishing Group |
publishDate |
2018 |
url |
http://hdl.handle.net/1721.1/117414 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Nature |
op_relation |
http://dx.doi.org/10.1038/s41396-018-0245-3 ISME Journal 1751-7362 1751-7370 http://hdl.handle.net/1721.1/117414 Bruto, Maxime et al. “Ancestral Gene Acquisition as the Key to Virulence Potential in Environmental Vibrio Populations.” The ISME Journal (August 2018) © 2018 The Authors orcid:0000-0001-9296-3733 |
op_rights |
Creative Commons Attribution 4.0 International License http://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1038/s41396-018-0245-3 |
container_title |
The ISME Journal |
container_volume |
12 |
container_issue |
12 |
container_start_page |
2954 |
op_container_end_page |
2966 |
_version_ |
1768386029415301120 |