Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum
Flavobacterium psychrophilum is a Gram-negative bacteria that produce proteins that cause tissue degradation leading to diseases like bacterial cold-water diseases (BCWD) and rainbow trout fry syndrome (RTFS), among salmonids. The manifestation of these infections includes skin ulcers, necrotic myos...
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Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato
2022
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ftminnesotastuni:oai:cornerstone.lib.mnsu.edu:urs-3729 2023-05-15T15:32:38+02:00 Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum Marsani, Ritika Beveridge, Carly 2022-04-12T21:00:00Z https://cornerstone.lib.mnsu.edu/urs/2022/poster-session-02/19 unknown Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato https://cornerstone.lib.mnsu.edu/urs/2022/poster-session-02/19 Undergraduate Research Symposium Bacteriology Environmental Microbiology and Microbial Ecology text 2022 ftminnesotastuni 2022-05-12T17:45:51Z Flavobacterium psychrophilum is a Gram-negative bacteria that produce proteins that cause tissue degradation leading to diseases like bacterial cold-water diseases (BCWD) and rainbow trout fry syndrome (RTFS), among salmonids. The manifestation of these infections includes skin ulcers, necrotic myositis, septicemia, and exophthalmia. Bacterial cold-water diseases are prominent when water is 16oC and below. Among salmonids, F. psychrophilum mainly targets Coho salmon, Rainbow trout, Atlantic salmon, and Ayu. Adult fish are highly susceptible to necrotic lesion caused by BCWD whereas young fish is highly susceptible to hemorrhagic septicemia caused by RTFS. Global economic losses in aquaculture are caused due to BCWD and RTFS. Therefore, it is necessary to address the damages caused by infections caused by F. psychrophilum. The goal of this study is to develop genetic manipulation tools that can be used to understand the virulence mechanisms of F. psychrophilum. Specifically, we will construct helper plasmids carrying methyltransferase genes that can methylate and protect foreign DNA during conjugation between E. coli and F. psychrophilum. We have been cloning two methyltransferase genes from F. psychrophilum (acuIM and its downstream gene) into the pACYC184 plasmid. We plan to test if the helper plasmids carrying acuIM and its downstream methyltransferase would protect foreign DNA and increase the conjugation efficiency of F. psychrophilum. These tools will further allow us to generate avirulent or less virulent mutants that can be developed as live attenuated vaccines. Text Atlantic salmon Minnesota State University, Mankato: Cornerstone |
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Minnesota State University, Mankato: Cornerstone |
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Bacteriology Environmental Microbiology and Microbial Ecology |
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Bacteriology Environmental Microbiology and Microbial Ecology Marsani, Ritika Beveridge, Carly Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum |
topic_facet |
Bacteriology Environmental Microbiology and Microbial Ecology |
description |
Flavobacterium psychrophilum is a Gram-negative bacteria that produce proteins that cause tissue degradation leading to diseases like bacterial cold-water diseases (BCWD) and rainbow trout fry syndrome (RTFS), among salmonids. The manifestation of these infections includes skin ulcers, necrotic myositis, septicemia, and exophthalmia. Bacterial cold-water diseases are prominent when water is 16oC and below. Among salmonids, F. psychrophilum mainly targets Coho salmon, Rainbow trout, Atlantic salmon, and Ayu. Adult fish are highly susceptible to necrotic lesion caused by BCWD whereas young fish is highly susceptible to hemorrhagic septicemia caused by RTFS. Global economic losses in aquaculture are caused due to BCWD and RTFS. Therefore, it is necessary to address the damages caused by infections caused by F. psychrophilum. The goal of this study is to develop genetic manipulation tools that can be used to understand the virulence mechanisms of F. psychrophilum. Specifically, we will construct helper plasmids carrying methyltransferase genes that can methylate and protect foreign DNA during conjugation between E. coli and F. psychrophilum. We have been cloning two methyltransferase genes from F. psychrophilum (acuIM and its downstream gene) into the pACYC184 plasmid. We plan to test if the helper plasmids carrying acuIM and its downstream methyltransferase would protect foreign DNA and increase the conjugation efficiency of F. psychrophilum. These tools will further allow us to generate avirulent or less virulent mutants that can be developed as live attenuated vaccines. |
format |
Text |
author |
Marsani, Ritika Beveridge, Carly |
author_facet |
Marsani, Ritika Beveridge, Carly |
author_sort |
Marsani, Ritika |
title |
Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum |
title_short |
Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum |
title_full |
Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum |
title_fullStr |
Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum |
title_full_unstemmed |
Development of Genetic Manipulation Techniques for the Fish Pathogen, Flavobacterium psychrophilum |
title_sort |
development of genetic manipulation techniques for the fish pathogen, flavobacterium psychrophilum |
publisher |
Cornerstone: A Collection of Scholarly and Creative Works for Minnesota State University, Mankato |
publishDate |
2022 |
url |
https://cornerstone.lib.mnsu.edu/urs/2022/poster-session-02/19 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Undergraduate Research Symposium |
op_relation |
https://cornerstone.lib.mnsu.edu/urs/2022/poster-session-02/19 |
_version_ |
1766363120063741952 |