Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean
Thesis (M.Sc.)--Memorial University of Newfoundland, 1995. Biology Bibliography: leaves 161-177. The Biolog™ MicroStation System was evaluated for its capacity to identify marine bacteria. The strains studied were Vibrionaceae type cultures and strains isolated from a seasonally-cold ocean. Using th...
Main Author: | |
---|---|
Other Authors: | |
Format: | Thesis |
Language: | English |
Published: |
1995
|
Subjects: | |
Online Access: | http://collections.mun.ca/cdm/ref/collection/theses3/id/37188 |
id |
ftmemorialunivdc:oai:collections.mun.ca:theses3/37188 |
---|---|
record_format |
openpolar |
spelling |
ftmemorialunivdc:oai:collections.mun.ca:theses3/37188 2023-05-15T17:23:32+02:00 Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean Noble, Lisa Dorothy Memorial University of Newfoundland. Dept. of Biology 1995 xiv, 178, [72] leaves Image/jpeg; Application/pdf http://collections.mun.ca/cdm/ref/collection/theses3/id/37188 eng eng Electronic Theses and Dissertations (28.81 MB) -- http://collections.mun.ca/PDFs/theses/Noble_LisaDorothy.pdf 76245788 http://collections.mun.ca/cdm/ref/collection/theses3/id/37188 The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries Biolog MicroStation System (Computer System) Vibrionaceae--Identification--Data processing Vibrionaceae--Classification--Data processing Text Electronic thesis or dissertation 1995 ftmemorialunivdc 2015-08-06T19:17:53Z Thesis (M.Sc.)--Memorial University of Newfoundland, 1995. Biology Bibliography: leaves 161-177. The Biolog™ MicroStation System was evaluated for its capacity to identify marine bacteria. The strains studied were Vibrionaceae type cultures and strains isolated from a seasonally-cold ocean. Using the Biolog system 54% of the Vibrionaceae strains were correctly identified, 18% of the strains were incorrectly identified, and 28% were not assigned an identity. Part of this study was to investigate ways in which the accuracy of the system might be improved. -- Marine bacteria require Na+ for growth. Biolog protocol was altered to bring these strains to a more optimal metabolic potential during tests. It was found that 62% of strains were more metabolically active when suspended in a marine cation supplement (MCS) plus yeast (MCSpY) . Twenty-four percent of the strains were more metabolically active when suspended in salts solutions containing MCS. MCS was used in the suspending salts when testing the regional strains because this solution encouraged high metabolic activity and had a comparable identification rate to the standard suspension solution which was saline. -- Approximately 36% of the regional strains were assigned a name by Biolog. Based on previous knowledge of the type of metabolism of the bacteria the names assigned were probably incorrect. It was concluded that the Biolog system was an inadequate identification system for Vibrionaceae. A classification procedure based on data collected by the Biolog system was not significantly different (p- value < 0.01) from a classification based on data produced from traditional or classical bacteriological tests. This indicated that the Biolog microbial identification system could be useful for identification and classification provided that the users included reference cultures in the studies and subsequently created their own data bases. A classification meeting these criteria was produced during the course of this study. Thesis Newfoundland studies University of Newfoundland Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
institution |
Open Polar |
collection |
Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
op_collection_id |
ftmemorialunivdc |
language |
English |
topic |
Biolog MicroStation System (Computer System) Vibrionaceae--Identification--Data processing Vibrionaceae--Classification--Data processing |
spellingShingle |
Biolog MicroStation System (Computer System) Vibrionaceae--Identification--Data processing Vibrionaceae--Classification--Data processing Noble, Lisa Dorothy Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean |
topic_facet |
Biolog MicroStation System (Computer System) Vibrionaceae--Identification--Data processing Vibrionaceae--Classification--Data processing |
description |
Thesis (M.Sc.)--Memorial University of Newfoundland, 1995. Biology Bibliography: leaves 161-177. The Biolog™ MicroStation System was evaluated for its capacity to identify marine bacteria. The strains studied were Vibrionaceae type cultures and strains isolated from a seasonally-cold ocean. Using the Biolog system 54% of the Vibrionaceae strains were correctly identified, 18% of the strains were incorrectly identified, and 28% were not assigned an identity. Part of this study was to investigate ways in which the accuracy of the system might be improved. -- Marine bacteria require Na+ for growth. Biolog protocol was altered to bring these strains to a more optimal metabolic potential during tests. It was found that 62% of strains were more metabolically active when suspended in a marine cation supplement (MCS) plus yeast (MCSpY) . Twenty-four percent of the strains were more metabolically active when suspended in salts solutions containing MCS. MCS was used in the suspending salts when testing the regional strains because this solution encouraged high metabolic activity and had a comparable identification rate to the standard suspension solution which was saline. -- Approximately 36% of the regional strains were assigned a name by Biolog. Based on previous knowledge of the type of metabolism of the bacteria the names assigned were probably incorrect. It was concluded that the Biolog system was an inadequate identification system for Vibrionaceae. A classification procedure based on data collected by the Biolog system was not significantly different (p- value < 0.01) from a classification based on data produced from traditional or classical bacteriological tests. This indicated that the Biolog microbial identification system could be useful for identification and classification provided that the users included reference cultures in the studies and subsequently created their own data bases. A classification meeting these criteria was produced during the course of this study. |
author2 |
Memorial University of Newfoundland. Dept. of Biology |
format |
Thesis |
author |
Noble, Lisa Dorothy |
author_facet |
Noble, Lisa Dorothy |
author_sort |
Noble, Lisa Dorothy |
title |
Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean |
title_short |
Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean |
title_full |
Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean |
title_fullStr |
Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean |
title_full_unstemmed |
Use of the Biolog MicroStation System to classify and identify Vibrionaceae bacteria from a seasonally-cold ocean |
title_sort |
use of the biolog microstation system to classify and identify vibrionaceae bacteria from a seasonally-cold ocean |
publishDate |
1995 |
url |
http://collections.mun.ca/cdm/ref/collection/theses3/id/37188 |
genre |
Newfoundland studies University of Newfoundland |
genre_facet |
Newfoundland studies University of Newfoundland |
op_source |
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries |
op_relation |
Electronic Theses and Dissertations (28.81 MB) -- http://collections.mun.ca/PDFs/theses/Noble_LisaDorothy.pdf 76245788 http://collections.mun.ca/cdm/ref/collection/theses3/id/37188 |
op_rights |
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. |
_version_ |
1766113065763340288 |