Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture
Thesis (M.Sc.) -- Memorial University of Newfoundland, 1990. Medicine Bibliography: leaves 134-143 At least 4 classes of voltage activated potassium currents have been identified on adult frog muscle using macroscopic voltage clamp techniques. This thesis deals with the identification and characteri...
Main Author: | |
---|---|
Other Authors: | |
Format: | Thesis |
Language: | English |
Published: |
1990
|
Subjects: | |
Online Access: | http://collections.mun.ca/cdm/ref/collection/theses3/id/35376 |
id |
ftmemorialunivdc:oai:collections.mun.ca:theses3/35376 |
---|---|
record_format |
openpolar |
spelling |
ftmemorialunivdc:oai:collections.mun.ca:theses3/35376 2023-05-15T17:23:32+02:00 Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture Gilbert, Robert William. Memorial University of Newfoundland. Faculty of Medicine 1990 xii, 145 leaves : ill. Image/jpeg; Application/pdf http://collections.mun.ca/cdm/ref/collection/theses3/id/35376 Eng eng Electronic Theses and Dissertations (13.93 MB) -- http://collections.mun.ca/PDFs/theses/Gilbert_RobertWilliam.pdf 76072861 http://collections.mun.ca/cdm/ref/collection/theses3/id/35376 The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries Potassium channels Myogenesis Voltage-clamp techniques (Electrophysiology) Striated muscle--Electric properties Xenopus--embryology Muscles Text Electronic thesis or dissertation 1990 ftmemorialunivdc 2015-08-06T19:17:50Z Thesis (M.Sc.) -- Memorial University of Newfoundland, 1990. Medicine Bibliography: leaves 134-143 At least 4 classes of voltage activated potassium currents have been identified on adult frog muscle using macroscopic voltage clamp techniques. This thesis deals with the identification and characterization of two potassium conductances present in the plasma membrane of embryonic Xenopus muscle cells growing in 24 hour old cultures. The tight seal whole cell recording technique was used in this study. One of the currents identified was a hyperpolarization gated inward potassium current thought to be mediated through anomalous rectifying potassium channels. This current activated when the membrane potential was hyperpolarized to values negative to the equilibrium potential for potassium and could be blocked by the external application of 1mM cesium chloride. A second depolarization activated outward current possesses characteristics similar to the delayed rectifier potassium current described on adult frog skeletal muscle ???rations. This conductance activated upon membrane depolarizations positive to membrane potentials of -30mV and could be reduced by the external application of 50mM Tetraethylammonium. Kinetic and pharmacological properties of these currents have been examined. Thesis Newfoundland studies University of Newfoundland Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
institution |
Open Polar |
collection |
Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
op_collection_id |
ftmemorialunivdc |
language |
English |
topic |
Potassium channels Myogenesis Voltage-clamp techniques (Electrophysiology) Striated muscle--Electric properties Xenopus--embryology Muscles |
spellingShingle |
Potassium channels Myogenesis Voltage-clamp techniques (Electrophysiology) Striated muscle--Electric properties Xenopus--embryology Muscles Gilbert, Robert William. Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
topic_facet |
Potassium channels Myogenesis Voltage-clamp techniques (Electrophysiology) Striated muscle--Electric properties Xenopus--embryology Muscles |
description |
Thesis (M.Sc.) -- Memorial University of Newfoundland, 1990. Medicine Bibliography: leaves 134-143 At least 4 classes of voltage activated potassium currents have been identified on adult frog muscle using macroscopic voltage clamp techniques. This thesis deals with the identification and characterization of two potassium conductances present in the plasma membrane of embryonic Xenopus muscle cells growing in 24 hour old cultures. The tight seal whole cell recording technique was used in this study. One of the currents identified was a hyperpolarization gated inward potassium current thought to be mediated through anomalous rectifying potassium channels. This current activated when the membrane potential was hyperpolarized to values negative to the equilibrium potential for potassium and could be blocked by the external application of 1mM cesium chloride. A second depolarization activated outward current possesses characteristics similar to the delayed rectifier potassium current described on adult frog skeletal muscle ???rations. This conductance activated upon membrane depolarizations positive to membrane potentials of -30mV and could be reduced by the external application of 50mM Tetraethylammonium. Kinetic and pharmacological properties of these currents have been examined. |
author2 |
Memorial University of Newfoundland. Faculty of Medicine |
format |
Thesis |
author |
Gilbert, Robert William. |
author_facet |
Gilbert, Robert William. |
author_sort |
Gilbert, Robert William. |
title |
Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
title_short |
Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
title_full |
Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
title_fullStr |
Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
title_full_unstemmed |
Two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
title_sort |
two voltage gated potassium currents on embryonic xenopus skeletal muscle cells growing in culture |
publishDate |
1990 |
url |
http://collections.mun.ca/cdm/ref/collection/theses3/id/35376 |
genre |
Newfoundland studies University of Newfoundland |
genre_facet |
Newfoundland studies University of Newfoundland |
op_source |
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries |
op_relation |
Electronic Theses and Dissertations (13.93 MB) -- http://collections.mun.ca/PDFs/theses/Gilbert_RobertWilliam.pdf 76072861 http://collections.mun.ca/cdm/ref/collection/theses3/id/35376 |
op_rights |
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. |
_version_ |
1766113064548040704 |