Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss
Thesis (M.Sc.)--Memorial University of Newfoundland. Biochemistry Bibliography: leaves 129-143 Scytalidium acidophilum was cultured in various peat based substrates to determine which one would produce the highest concentration of biomass. Peat was hydrolyzed individually with sulfuric, hydrochloric...
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ftmemorialunivdc:oai:collections.mun.ca:theses3/147026 2023-05-15T17:23:32+02:00 Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss Chintalapati, Sharada P. Memorial University of Newfoundland. Dept. of Biochemistry 1988 xvii, 154 leaves : ill. Image/jpeg; Application/pdf http://collections.mun.ca/cdm/ref/collection/theses3/id/147026 eng eng Electronic Theses and Dissertations (19.10 MB) -- http://collections.mun.ca/PDFs/theses/Chintalapati_SharadaP.pdf 76082971 http://collections.mun.ca/cdm/ref/collection/theses3/id/147026 The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries Scytalidium acidophilum--Growth Peat Text Electronic thesis or dissertation 1988 ftmemorialunivdc 2015-08-06T19:20:21Z Thesis (M.Sc.)--Memorial University of Newfoundland. Biochemistry Bibliography: leaves 129-143 Scytalidium acidophilum was cultured in various peat based substrates to determine which one would produce the highest concentration of biomass. Peat was hydrolyzed individually with sulfuric, hydrochloric, nitric and phosphoric acids and the hydrolysates used as substrates to prepare the media for culturing the fungus. The proximate composition of the hydrolysates were determined. The H2SO4 hydrolysate of peat was the richest in carbohydrate concentration. The constituent monosaccharides in the peat hydrolysates were determined by High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). Hexoses constituted nore than 50 % of the total reducing sugars present in the peat hydrolysates. -- Preliminary experiments with synthetic media showed that 5% (v/v) inoculum ratio produces the highest growth of the fungus. Growth of the fungus on the media containing different carbon sources indicated that hexoses were better utilized by the fungus than pentoses. -- Undiluted and water-diluted peat hydrolysates were used for culturing the fungus. Peat hydrolyzed with sulfuric acid and diluted 1:1 with water produced the highest growth parameters of the fungus. Supplementation of the hydrolysates with 3 g/L yeast extract and 0.4 g/L magnesium sulfate improved the growth of the fungus compared to the other nutritional additives tested. -- The effects of components of peat on the growth of the fungus were investigated. A study involving the removal of one peat component (modified peats) to avoid the potential inhibitory effect of peat is not a viable method of increasing the biomass production. A comparison of non-modified and modified peat hydrolysates showed that modification of peat results in a loss of nutrients. -- The nutrient supplementation of modified peat hydrolysates caused no statistically significant increases (P < 0.05) in the growth parameters as compared with those obtained when non-supplemented modified peat hydrolysates were used. -- Fractionation of humic substances of peat was completed to identify the growth inhibiting components. The fulvic acid fraction was found to show inhibitory effect on the growth of the fungus while the humic acids fraction was stimulatory. -- Hydrolysates obtained from peat-fish offal compost were also used as sub-strates for the growth of the fungus. Growth curves have been constructed by growing S. acidophilum in peat and compost hydrolysates. The composition of biomass produced on peat hydrolysate was determined. Thesis Newfoundland studies University of Newfoundland Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
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Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
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ftmemorialunivdc |
language |
English |
topic |
Scytalidium acidophilum--Growth Peat |
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Scytalidium acidophilum--Growth Peat Chintalapati, Sharada P. Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss |
topic_facet |
Scytalidium acidophilum--Growth Peat |
description |
Thesis (M.Sc.)--Memorial University of Newfoundland. Biochemistry Bibliography: leaves 129-143 Scytalidium acidophilum was cultured in various peat based substrates to determine which one would produce the highest concentration of biomass. Peat was hydrolyzed individually with sulfuric, hydrochloric, nitric and phosphoric acids and the hydrolysates used as substrates to prepare the media for culturing the fungus. The proximate composition of the hydrolysates were determined. The H2SO4 hydrolysate of peat was the richest in carbohydrate concentration. The constituent monosaccharides in the peat hydrolysates were determined by High Performance Liquid Chromatography (HPLC) and Gas Chromatography (GC). Hexoses constituted nore than 50 % of the total reducing sugars present in the peat hydrolysates. -- Preliminary experiments with synthetic media showed that 5% (v/v) inoculum ratio produces the highest growth of the fungus. Growth of the fungus on the media containing different carbon sources indicated that hexoses were better utilized by the fungus than pentoses. -- Undiluted and water-diluted peat hydrolysates were used for culturing the fungus. Peat hydrolyzed with sulfuric acid and diluted 1:1 with water produced the highest growth parameters of the fungus. Supplementation of the hydrolysates with 3 g/L yeast extract and 0.4 g/L magnesium sulfate improved the growth of the fungus compared to the other nutritional additives tested. -- The effects of components of peat on the growth of the fungus were investigated. A study involving the removal of one peat component (modified peats) to avoid the potential inhibitory effect of peat is not a viable method of increasing the biomass production. A comparison of non-modified and modified peat hydrolysates showed that modification of peat results in a loss of nutrients. -- The nutrient supplementation of modified peat hydrolysates caused no statistically significant increases (P < 0.05) in the growth parameters as compared with those obtained when non-supplemented modified peat hydrolysates were used. -- Fractionation of humic substances of peat was completed to identify the growth inhibiting components. The fulvic acid fraction was found to show inhibitory effect on the growth of the fungus while the humic acids fraction was stimulatory. -- Hydrolysates obtained from peat-fish offal compost were also used as sub-strates for the growth of the fungus. Growth curves have been constructed by growing S. acidophilum in peat and compost hydrolysates. The composition of biomass produced on peat hydrolysate was determined. |
author2 |
Memorial University of Newfoundland. Dept. of Biochemistry |
format |
Thesis |
author |
Chintalapati, Sharada P. |
author_facet |
Chintalapati, Sharada P. |
author_sort |
Chintalapati, Sharada P. |
title |
Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss |
title_short |
Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss |
title_full |
Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss |
title_fullStr |
Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss |
title_full_unstemmed |
Studies on the growth of fungus Scytalidium acidophilum in hydrolysates from sphagnum peat moss |
title_sort |
studies on the growth of fungus scytalidium acidophilum in hydrolysates from sphagnum peat moss |
publishDate |
1988 |
url |
http://collections.mun.ca/cdm/ref/collection/theses3/id/147026 |
genre |
Newfoundland studies University of Newfoundland |
genre_facet |
Newfoundland studies University of Newfoundland |
op_source |
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries |
op_relation |
Electronic Theses and Dissertations (19.10 MB) -- http://collections.mun.ca/PDFs/theses/Chintalapati_SharadaP.pdf 76082971 http://collections.mun.ca/cdm/ref/collection/theses3/id/147026 |
op_rights |
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. |
_version_ |
1766113150272274432 |