Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression
Thesis (M.Sc.)--Memorial University of Newfoundland, 1987. Medicine Bibliography: leaves 103-116. Carcinoembryonic antigen (CEA), a human tumor-associated marker for many epithelial tumors, has been well characterized immunologically and structurally but its biological function(s) remains a mystery....
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ftmemorialunivdc:oai:collections.mun.ca:theses2/44259 2023-05-15T17:23:30+02:00 Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression Sutherland, Peter Michael, 1963- Memorial University of Newfoundland. Faculty of Medicine 1987 x, 136 leaves : ill., plates. Image/jpeg; Application/pdf http://collections.mun.ca/cdm/ref/collection/theses2/id/44259 Eng eng Electronic Theses and Dissertations (18.87 MB) -- http://collections.mun.ca/PDFs/theses/Sutherland_PeterMichael.pdf 75414524 http://collections.mun.ca/cdm/ref/collection/theses2/id/44259 The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries CEA (Oncology) Somatic hybrids Gene mapping Text Electronic thesis or dissertation 1987 ftmemorialunivdc 2015-08-06T19:16:35Z Thesis (M.Sc.)--Memorial University of Newfoundland, 1987. Medicine Bibliography: leaves 103-116. Carcinoembryonic antigen (CEA), a human tumor-associated marker for many epithelial tumors, has been well characterized immunologically and structurally but its biological function(s) remains a mystery. The objectives of this investigation were to produce interspecific somatic cell hybrids between human colorectal, CEA expressing/secreting, cancer cell lines (LS174T, SKC01 and HT29) and mouse cell lines (NS1 SP2/0, RAG, PG19 and STO) and to characterize the resultant hybrids for CEA expression/secretion with the longer term goal of assigning the CEA gene(s) to a particular chromosome. -- Fusions were performed using a polyethylene glycol (PEG) suspension technique and hybrids were isolated by hypoxanthine-aminopterin-thymidine/ouabain double selection and characterized for CEA expression/secretion in specific CEA immunoassays. Forty-eight fusions were performed (43, monolayer-suspension; 5, monolayer-monolayer) producing 344 hybrid colonies of the following types (SKCO1xRAG, SKCO1xSTO, HT29xRAG, HT29xSTO and HT29xNS1). Chromosomal analysis by Giemsa differential staining confirmed that these fusion products were definite hybrids containing human and mouse chromosomes. - This study has demonstrated (1) the successful production of human-mouse somatic cell hybrids, (2) that fusion of monolayer-monolayer cell lines resulted in a greater yield of hybrids and (3) that none of the hybrids obtained showed high levels of CEA expression/secretion, probably because the RAG and STO cell lines were nonpermissive fusion partners. Thesis Newfoundland studies University of Newfoundland Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
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Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
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English |
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CEA (Oncology) Somatic hybrids Gene mapping |
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CEA (Oncology) Somatic hybrids Gene mapping Sutherland, Peter Michael, 1963- Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
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CEA (Oncology) Somatic hybrids Gene mapping |
description |
Thesis (M.Sc.)--Memorial University of Newfoundland, 1987. Medicine Bibliography: leaves 103-116. Carcinoembryonic antigen (CEA), a human tumor-associated marker for many epithelial tumors, has been well characterized immunologically and structurally but its biological function(s) remains a mystery. The objectives of this investigation were to produce interspecific somatic cell hybrids between human colorectal, CEA expressing/secreting, cancer cell lines (LS174T, SKC01 and HT29) and mouse cell lines (NS1 SP2/0, RAG, PG19 and STO) and to characterize the resultant hybrids for CEA expression/secretion with the longer term goal of assigning the CEA gene(s) to a particular chromosome. -- Fusions were performed using a polyethylene glycol (PEG) suspension technique and hybrids were isolated by hypoxanthine-aminopterin-thymidine/ouabain double selection and characterized for CEA expression/secretion in specific CEA immunoassays. Forty-eight fusions were performed (43, monolayer-suspension; 5, monolayer-monolayer) producing 344 hybrid colonies of the following types (SKCO1xRAG, SKCO1xSTO, HT29xRAG, HT29xSTO and HT29xNS1). Chromosomal analysis by Giemsa differential staining confirmed that these fusion products were definite hybrids containing human and mouse chromosomes. - This study has demonstrated (1) the successful production of human-mouse somatic cell hybrids, (2) that fusion of monolayer-monolayer cell lines resulted in a greater yield of hybrids and (3) that none of the hybrids obtained showed high levels of CEA expression/secretion, probably because the RAG and STO cell lines were nonpermissive fusion partners. |
author2 |
Memorial University of Newfoundland. Faculty of Medicine |
format |
Thesis |
author |
Sutherland, Peter Michael, 1963- |
author_facet |
Sutherland, Peter Michael, 1963- |
author_sort |
Sutherland, Peter Michael, 1963- |
title |
Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
title_short |
Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
title_full |
Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
title_fullStr |
Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
title_full_unstemmed |
Production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
title_sort |
production of human-mouse somatic cell hybrids and characterization for carcinoembryonic antigen expression |
publishDate |
1987 |
url |
http://collections.mun.ca/cdm/ref/collection/theses2/id/44259 |
genre |
Newfoundland studies University of Newfoundland |
genre_facet |
Newfoundland studies University of Newfoundland |
op_source |
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries |
op_relation |
Electronic Theses and Dissertations (18.87 MB) -- http://collections.mun.ca/PDFs/theses/Sutherland_PeterMichael.pdf 75414524 http://collections.mun.ca/cdm/ref/collection/theses2/id/44259 |
op_rights |
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. |
_version_ |
1766112815171502080 |