Subcellular compartmentalization of mRNAs
Thesis (M.Sc.)--Memorial University of Newfoundland, 1986. Medicine Bibliography: leaves 75-84. The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts an...
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ftmemorialunivdc:oai:collections.mun.ca:theses2/220087 2023-05-15T17:23:31+02:00 Subcellular compartmentalization of mRNAs Bagchi, Tamishraha Memorial University of Newfoundland. Faculty of Medicine 1986 viii, 84 leaves : ill. Image/jpeg; Application/pdf http://collections.mun.ca/cdm/ref/collection/theses2/id/220087 Eng eng Electronic Theses and Dissertations (12.22 MB) -- http://collections.mun.ca/PDFs/theses/Bagchi_Tamishraha.pdf 75380718 http://collections.mun.ca/cdm/ref/collection/theses2/id/220087 The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries Cytoskeleton Cell compartmentation Proteins--Synthesis RNA Messenger Text Electronic thesis or dissertation 1986 ftmemorialunivdc 2015-08-06T19:17:26Z Thesis (M.Sc.)--Memorial University of Newfoundland, 1986. Medicine Bibliography: leaves 75-84. The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. The role of the cytoskeleton in compartmentalization of specific mRNPs in myoblasts and myotubes was investigated. -- mRNPs in polysomal arrays were primarily associated with the cytoskeleton. In addition, the cytoskeletal fraction also contained mRNA in the form of free mRNPs. Therefore, the association of mRNA with the cytoskeleton did not seem to depend on the presence of ribosomes for attachment. Furthermore, analysis of specific mRNAs in the various subcellular fractions of myoblasts and myotubes revealed differences in the distribution pattern of these mRNAs. -- The effects of depolymerizing the microfilaments with cytochalasin B in myoblasts, was investigated. Treatment of myoblasts with cytochalasin B did not result in movement of ribosomes or specific mRNPs from the cytoskeletal fraction to the soluble fraction. This indicates that in L6 myoblasts, ribosomes and mRNPs are not associated with microfilaments. In addition, it was observed that cytochalasin B inhibited the incorporation of precursor into RNA and not protein. The effect on RNA synthesis, however, was due to an inhibition of uptake of precursor uridine, which was found to be reversible. Thesis Newfoundland studies University of Newfoundland Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
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Memorial University of Newfoundland: Digital Archives Initiative (DAI) |
op_collection_id |
ftmemorialunivdc |
language |
English |
topic |
Cytoskeleton Cell compartmentation Proteins--Synthesis RNA Messenger |
spellingShingle |
Cytoskeleton Cell compartmentation Proteins--Synthesis RNA Messenger Bagchi, Tamishraha Subcellular compartmentalization of mRNAs |
topic_facet |
Cytoskeleton Cell compartmentation Proteins--Synthesis RNA Messenger |
description |
Thesis (M.Sc.)--Memorial University of Newfoundland, 1986. Medicine Bibliography: leaves 75-84. The importance of the cytoskeleton in protein synthesis was studied in differentiating L6 rat myoblasts. Soluble and cytoskeletal fractions obtained after gentle, non-ionic detergent lysis of myoblasts and myotubes were analysed for the presence of ribosomes and mRNPs. The role of the cytoskeleton in compartmentalization of specific mRNPs in myoblasts and myotubes was investigated. -- mRNPs in polysomal arrays were primarily associated with the cytoskeleton. In addition, the cytoskeletal fraction also contained mRNA in the form of free mRNPs. Therefore, the association of mRNA with the cytoskeleton did not seem to depend on the presence of ribosomes for attachment. Furthermore, analysis of specific mRNAs in the various subcellular fractions of myoblasts and myotubes revealed differences in the distribution pattern of these mRNAs. -- The effects of depolymerizing the microfilaments with cytochalasin B in myoblasts, was investigated. Treatment of myoblasts with cytochalasin B did not result in movement of ribosomes or specific mRNPs from the cytoskeletal fraction to the soluble fraction. This indicates that in L6 myoblasts, ribosomes and mRNPs are not associated with microfilaments. In addition, it was observed that cytochalasin B inhibited the incorporation of precursor into RNA and not protein. The effect on RNA synthesis, however, was due to an inhibition of uptake of precursor uridine, which was found to be reversible. |
author2 |
Memorial University of Newfoundland. Faculty of Medicine |
format |
Thesis |
author |
Bagchi, Tamishraha |
author_facet |
Bagchi, Tamishraha |
author_sort |
Bagchi, Tamishraha |
title |
Subcellular compartmentalization of mRNAs |
title_short |
Subcellular compartmentalization of mRNAs |
title_full |
Subcellular compartmentalization of mRNAs |
title_fullStr |
Subcellular compartmentalization of mRNAs |
title_full_unstemmed |
Subcellular compartmentalization of mRNAs |
title_sort |
subcellular compartmentalization of mrnas |
publishDate |
1986 |
url |
http://collections.mun.ca/cdm/ref/collection/theses2/id/220087 |
genre |
Newfoundland studies University of Newfoundland |
genre_facet |
Newfoundland studies University of Newfoundland |
op_source |
Paper copy kept in the Centre for Newfoundland Studies, Memorial University Libraries |
op_relation |
Electronic Theses and Dissertations (12.22 MB) -- http://collections.mun.ca/PDFs/theses/Bagchi_Tamishraha.pdf 75380718 http://collections.mun.ca/cdm/ref/collection/theses2/id/220087 |
op_rights |
The author retains copyright ownership and moral rights in this thesis. Neither the thesis nor substantial extracts from it may be printed or otherwise reproduced without the author's permission. |
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1766112980830781440 |