Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112)
This study will present an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted Lipid A that is obtained from the native LPS extracts isolated from the marine Gram-negative bacteria Aeromonas Salmonicida (SJ-112). It is known that the surface antigen lipopolysacc...
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Memorial University of Newfoundland
2015
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ftmemorialuniv:oai:research.library.mun.ca:8511 2023-10-01T03:54:47+02:00 Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) Alarabi, Faisal 2015-06 application/pdf https://research.library.mun.ca/8511/ https://research.library.mun.ca/8511/1/thesis.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/8511/1/thesis.pdf Alarabi, Faisal <https://research.library.mun.ca/view/creator_az/Alarabi=3AFaisal=3A=3A.html> (2015) Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112). Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2015 ftmemorialuniv 2023-09-03T06:46:57Z This study will present an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted Lipid A that is obtained from the native LPS extracts isolated from the marine Gram-negative bacteria Aeromonas Salmonicida (SJ-112). It is known that the surface antigen lipopolysaccarides (LPS) SJ-112 infect various fish species (Atlantic salmon and cod) which are cultivated in aquaculture ventures. The exact molecular structure of the Lipid A has not yet been precisely established. This thesis will present the mass spectrometric fingerprint identification and structural elucidation of the Lipid A from A. Salmonicida, which are carried out by using mass spectrometry techniques namely, electrospray ionization tandem mass spectrometry (ESI-MS/MS) using an FT-ICR instrument and matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS) using a TOF/TOF instrument. The concomitant uses of high-energy (CID-MS/MS) and low-energy collision induced dissociation (CID-MS/MS) analysis were also used to elucidate the MS/MS fingerprints of this complex biomolecule and can be effectively used for any quantitative or qualitative studies. Thesis Atlantic salmon Memorial University of Newfoundland: Research Repository |
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Memorial University of Newfoundland: Research Repository |
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ftmemorialuniv |
language |
English |
description |
This study will present an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted Lipid A that is obtained from the native LPS extracts isolated from the marine Gram-negative bacteria Aeromonas Salmonicida (SJ-112). It is known that the surface antigen lipopolysaccarides (LPS) SJ-112 infect various fish species (Atlantic salmon and cod) which are cultivated in aquaculture ventures. The exact molecular structure of the Lipid A has not yet been precisely established. This thesis will present the mass spectrometric fingerprint identification and structural elucidation of the Lipid A from A. Salmonicida, which are carried out by using mass spectrometry techniques namely, electrospray ionization tandem mass spectrometry (ESI-MS/MS) using an FT-ICR instrument and matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS) using a TOF/TOF instrument. The concomitant uses of high-energy (CID-MS/MS) and low-energy collision induced dissociation (CID-MS/MS) analysis were also used to elucidate the MS/MS fingerprints of this complex biomolecule and can be effectively used for any quantitative or qualitative studies. |
format |
Thesis |
author |
Alarabi, Faisal |
spellingShingle |
Alarabi, Faisal Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) |
author_facet |
Alarabi, Faisal |
author_sort |
Alarabi, Faisal |
title |
Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) |
title_short |
Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) |
title_full |
Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) |
title_fullStr |
Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) |
title_full_unstemmed |
Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112) |
title_sort |
tandem mass spectrometric analysis of bacterial lipid a of aeromonas salmonicida (sj-112) |
publisher |
Memorial University of Newfoundland |
publishDate |
2015 |
url |
https://research.library.mun.ca/8511/ https://research.library.mun.ca/8511/1/thesis.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://research.library.mun.ca/8511/1/thesis.pdf Alarabi, Faisal <https://research.library.mun.ca/view/creator_az/Alarabi=3AFaisal=3A=3A.html> (2015) Tandem mass spectrometric analysis of bacterial lipid A of Aeromonas Salmonicida (SJ-112). Masters thesis, Memorial University of Newfoundland. |
op_rights |
thesis_license |
_version_ |
1778522697044590592 |