Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety
This study presents an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted lipid A obtained from the native LPS extracts isolated from Aeromonas Liquefaciencs. This LPS SJ-19 is known to infect various fish species (Atlantic salmon and cod) which are cultivated...
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Memorial University of Newfoundland
2014
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ftmemorialuniv:oai:research.library.mun.ca:8334 2023-10-01T03:54:47+02:00 Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety Allehyane, Bashaeer 2014-09-15 application/pdf https://research.library.mun.ca/8334/ https://research.library.mun.ca/8334/1/thesis.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/8334/1/thesis.pdf Allehyane, Bashaeer <https://research.library.mun.ca/view/creator_az/Allehyane=3ABashaeer=3A=3A.html> (2014) Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety. Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2014 ftmemorialuniv 2023-09-03T06:46:51Z This study presents an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted lipid A obtained from the native LPS extracts isolated from Aeromonas Liquefaciencs. This LPS SJ-19 is known to infect various fish species (Atlantic salmon and cod) which are cultivated in aquaculture ventures. The exact molecular structure of the lipid A and the core oligosaccharide region of this Rough bacterium have not yet been precisely established. This thesis presents the mass spectrometric fingerprint identification and structural elucidation of the lipid A from A. Liquefaciencs, which was accomplished via the use of electrospray ionization tandem mass spectrometry (ESI-MS/MS using FTICR, QIT, QHQ-MS/MS) and matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS). In this work, tandem-in-time and tandem-in-space mass spectrometry was chosen for the structural elucidation of the lipid A isolated from A. Liquefaciencs SJ-19. The concomitant uses of high-energy and low-energy collision induced dissociation (CIDMS/ MS) analysis were also used to elucidate the MS/MS fingerprints of this complex biomolecule and can be effectively used for any quantitative or qualitative studies. In addition, a novel MS/MS study of a series of 2-acylated-2-deoxy-Dgluosamine glycosyl donors is also presented using ESI-MS/MS tandem-in-space and tandem-in-time mass spectrometry. Thesis Atlantic salmon Memorial University of Newfoundland: Research Repository |
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Memorial University of Newfoundland: Research Repository |
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English |
description |
This study presents an interpretation of the mass spectrometry gas-phase fragmentation patterns of the extracted lipid A obtained from the native LPS extracts isolated from Aeromonas Liquefaciencs. This LPS SJ-19 is known to infect various fish species (Atlantic salmon and cod) which are cultivated in aquaculture ventures. The exact molecular structure of the lipid A and the core oligosaccharide region of this Rough bacterium have not yet been precisely established. This thesis presents the mass spectrometric fingerprint identification and structural elucidation of the lipid A from A. Liquefaciencs, which was accomplished via the use of electrospray ionization tandem mass spectrometry (ESI-MS/MS using FTICR, QIT, QHQ-MS/MS) and matrix assisted laser desorption ionization tandem mass spectrometry (MALDI-MS/MS). In this work, tandem-in-time and tandem-in-space mass spectrometry was chosen for the structural elucidation of the lipid A isolated from A. Liquefaciencs SJ-19. The concomitant uses of high-energy and low-energy collision induced dissociation (CIDMS/ MS) analysis were also used to elucidate the MS/MS fingerprints of this complex biomolecule and can be effectively used for any quantitative or qualitative studies. In addition, a novel MS/MS study of a series of 2-acylated-2-deoxy-Dgluosamine glycosyl donors is also presented using ESI-MS/MS tandem-in-space and tandem-in-time mass spectrometry. |
format |
Thesis |
author |
Allehyane, Bashaeer |
spellingShingle |
Allehyane, Bashaeer Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety |
author_facet |
Allehyane, Bashaeer |
author_sort |
Allehyane, Bashaeer |
title |
Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety |
title_short |
Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety |
title_full |
Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety |
title_fullStr |
Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety |
title_full_unstemmed |
Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety |
title_sort |
tandem mass spectrometric analysis of bacterial lipid a and synthetic glycosyl donors containing the 2-amino-2-deoxy-d-glucosaminyl moiety |
publisher |
Memorial University of Newfoundland |
publishDate |
2014 |
url |
https://research.library.mun.ca/8334/ https://research.library.mun.ca/8334/1/thesis.pdf |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
https://research.library.mun.ca/8334/1/thesis.pdf Allehyane, Bashaeer <https://research.library.mun.ca/view/creator_az/Allehyane=3ABashaeer=3A=3A.html> (2014) Tandem mass spectrometric analysis of bacterial lipid A and synthetic glycosyl donors containing the 2-amino-2-deoxy-D-glucosaminyl moiety. Masters thesis, Memorial University of Newfoundland. |
op_rights |
thesis_license |
_version_ |
1778522715825635328 |