Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques

In recent years, there has been an increased concern over the appearance of endocrine dismpting chemicals (EDCs) in the aquatic environment due to their potential reproductive and early developmental toxicity to wildlife population. Municipal and industrial sewage effluents are considered as the maj...

Full description

Bibliographic Details
Main Author: Saravanabhavan, Gurusankar
Format: Thesis
Language:English
Published: Memorial University of Newfoundland 2003
Subjects:
Online Access:https://research.library.mun.ca/7084/
https://research.library.mun.ca/7084/1/Saravanabhavan_Gurusankar.pdf
https://research.library.mun.ca/7084/3/Saravanabhavan_Gurusankar.pdf
id ftmemorialuniv:oai:research.library.mun.ca:7084
record_format openpolar
spelling ftmemorialuniv:oai:research.library.mun.ca:7084 2023-10-01T03:57:38+02:00 Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques Saravanabhavan, Gurusankar 2003 application/pdf https://research.library.mun.ca/7084/ https://research.library.mun.ca/7084/1/Saravanabhavan_Gurusankar.pdf https://research.library.mun.ca/7084/3/Saravanabhavan_Gurusankar.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/7084/1/Saravanabhavan_Gurusankar.pdf https://research.library.mun.ca/7084/3/Saravanabhavan_Gurusankar.pdf Saravanabhavan, Gurusankar <https://research.library.mun.ca/view/creator_az/Saravanabhavan=3AGurusankar=3A=3A.html> (2003) Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques. Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2003 ftmemorialuniv 2023-09-03T06:46:13Z In recent years, there has been an increased concern over the appearance of endocrine dismpting chemicals (EDCs) in the aquatic environment due to their potential reproductive and early developmental toxicity to wildlife population. Municipal and industrial sewage effluents are considered as the major sources of EDCs. Among different classes of EDCs, natural and synthetic steroid estrogens have been identified as the most potent EDCs, as they can induce feminization in fish even at trace levels. Steroid hormones undergo extensive biodegradation during secondary treatment in municipal wastewater treatment processes. However, in Atlantic Canada only 50% of the population has municipal sewage treatment facilities. In many coastal towns and cities, including Halifax, Nova Scotia, and St. John's, Newfoundland & Labrador the raw sewage is directly discharged into harbours. Needless to say, sewage-related contaminants pose a serious threat to the marine ecosystem in these areas. -- Mussels are widely used to assess environmental impact of pollutants (metals and organics) in the marine environment. Mussels living near sewage outfalls may be exposed to constant levels of steroid estrogens through their food and respiration. This study examines whether these organisms can be used as a good bio-indicator for steroidal pollution. In this work, the analysis of steroid estrogens (estrone, estradiol, ethynylestradiol, diethylstilbestrol and mestranol) and fecal biomarker coprostanol in raw sewage, seawater and mussels collected from St. John's and Halifax harbours was undertaken. Two analytical methods based on GC- (ion trap) MS/MS were developed for mussel tissue and sewage analysis. A major factor in the success of method development was the removal of interferences of tissue matrix. Performance characteristics of these methods were evaluated using careful recovery experiments. Percentage recovery of analytes measured by spiking anaiyte standards in mussel and distilled water were >60% and >80% respectively. ... Thesis Newfoundland Memorial University of Newfoundland: Research Repository Newfoundland Canada
institution Open Polar
collection Memorial University of Newfoundland: Research Repository
op_collection_id ftmemorialuniv
language English
description In recent years, there has been an increased concern over the appearance of endocrine dismpting chemicals (EDCs) in the aquatic environment due to their potential reproductive and early developmental toxicity to wildlife population. Municipal and industrial sewage effluents are considered as the major sources of EDCs. Among different classes of EDCs, natural and synthetic steroid estrogens have been identified as the most potent EDCs, as they can induce feminization in fish even at trace levels. Steroid hormones undergo extensive biodegradation during secondary treatment in municipal wastewater treatment processes. However, in Atlantic Canada only 50% of the population has municipal sewage treatment facilities. In many coastal towns and cities, including Halifax, Nova Scotia, and St. John's, Newfoundland & Labrador the raw sewage is directly discharged into harbours. Needless to say, sewage-related contaminants pose a serious threat to the marine ecosystem in these areas. -- Mussels are widely used to assess environmental impact of pollutants (metals and organics) in the marine environment. Mussels living near sewage outfalls may be exposed to constant levels of steroid estrogens through their food and respiration. This study examines whether these organisms can be used as a good bio-indicator for steroidal pollution. In this work, the analysis of steroid estrogens (estrone, estradiol, ethynylestradiol, diethylstilbestrol and mestranol) and fecal biomarker coprostanol in raw sewage, seawater and mussels collected from St. John's and Halifax harbours was undertaken. Two analytical methods based on GC- (ion trap) MS/MS were developed for mussel tissue and sewage analysis. A major factor in the success of method development was the removal of interferences of tissue matrix. Performance characteristics of these methods were evaluated using careful recovery experiments. Percentage recovery of analytes measured by spiking anaiyte standards in mussel and distilled water were >60% and >80% respectively. ...
format Thesis
author Saravanabhavan, Gurusankar
spellingShingle Saravanabhavan, Gurusankar
Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques
author_facet Saravanabhavan, Gurusankar
author_sort Saravanabhavan, Gurusankar
title Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques
title_short Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques
title_full Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques
title_fullStr Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques
title_full_unstemmed Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques
title_sort analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using gc-ms techniques
publisher Memorial University of Newfoundland
publishDate 2003
url https://research.library.mun.ca/7084/
https://research.library.mun.ca/7084/1/Saravanabhavan_Gurusankar.pdf
https://research.library.mun.ca/7084/3/Saravanabhavan_Gurusankar.pdf
geographic Newfoundland
Canada
geographic_facet Newfoundland
Canada
genre Newfoundland
genre_facet Newfoundland
op_relation https://research.library.mun.ca/7084/1/Saravanabhavan_Gurusankar.pdf
https://research.library.mun.ca/7084/3/Saravanabhavan_Gurusankar.pdf
Saravanabhavan, Gurusankar <https://research.library.mun.ca/view/creator_az/Saravanabhavan=3AGurusankar=3A=3A.html> (2003) Analysis of steroid hormones as endocrine disruptors in sewage, seawater and mussels using GC-MS techniques. Masters thesis, Memorial University of Newfoundland.
op_rights thesis_license
_version_ 1778529483912904704