The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry
Modern proteomics tools allow for quick identification of proteins that may be present in aerosolized samples collected from snow crab processing. The coupling of electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has allowed for a rapid,...
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Memorial University of Newfoundland
2003
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ftmemorialuniv:oai:research.library.mun.ca:7056 2023-10-01T03:59:26+02:00 The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry Whiteway, Geoffrey 2003 application/pdf https://research.library.mun.ca/7056/ https://research.library.mun.ca/7056/1/Whiteway_Geoffrey.pdf https://research.library.mun.ca/7056/3/Whiteway_Geoffrey.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/7056/1/Whiteway_Geoffrey.pdf https://research.library.mun.ca/7056/3/Whiteway_Geoffrey.pdf Whiteway, Geoffrey <https://research.library.mun.ca/view/creator_az/Whiteway=3AGeoffrey=3A=3A.html> (2003) The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2003 ftmemorialuniv 2023-09-03T06:46:13Z Modern proteomics tools allow for quick identification of proteins that may be present in aerosolized samples collected from snow crab processing. The coupling of electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has allowed for a rapid, 2-3 day, technique for the identification of these proteins. The protein bands are excised from gel slices, the protein present digested using trypsin and then analyzed using a MALDI-TOF MS instrument to produce a peptide mass fingerprint. -- On-line database searching of the peptide mass fingerprints through Swiss-Prot or other database engines provide an efficient and effective means for protein identification, provided the protein has been sequenced. As well, unmatched peptide peaks can be analyzed through a post-translational modification database to help understand any chemical modifications that have occurred. -- This combinational approach has produced a rapid technique for the screening of possible proteins aerosolized during commercial snow crab processing, some of which are allergens. Using the Swiss-Prot database, tropomyosin was identified in both lab-produced condensate and an air filter sample collected from a commercial snow crab processing plant. Thesis Snow crab Memorial University of Newfoundland: Research Repository |
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Memorial University of Newfoundland: Research Repository |
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English |
description |
Modern proteomics tools allow for quick identification of proteins that may be present in aerosolized samples collected from snow crab processing. The coupling of electrophoresis and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) has allowed for a rapid, 2-3 day, technique for the identification of these proteins. The protein bands are excised from gel slices, the protein present digested using trypsin and then analyzed using a MALDI-TOF MS instrument to produce a peptide mass fingerprint. -- On-line database searching of the peptide mass fingerprints through Swiss-Prot or other database engines provide an efficient and effective means for protein identification, provided the protein has been sequenced. As well, unmatched peptide peaks can be analyzed through a post-translational modification database to help understand any chemical modifications that have occurred. -- This combinational approach has produced a rapid technique for the screening of possible proteins aerosolized during commercial snow crab processing, some of which are allergens. Using the Swiss-Prot database, tropomyosin was identified in both lab-produced condensate and an air filter sample collected from a commercial snow crab processing plant. |
format |
Thesis |
author |
Whiteway, Geoffrey |
spellingShingle |
Whiteway, Geoffrey The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
author_facet |
Whiteway, Geoffrey |
author_sort |
Whiteway, Geoffrey |
title |
The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
title_short |
The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
title_full |
The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
title_fullStr |
The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
title_full_unstemmed |
The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
title_sort |
identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry |
publisher |
Memorial University of Newfoundland |
publishDate |
2003 |
url |
https://research.library.mun.ca/7056/ https://research.library.mun.ca/7056/1/Whiteway_Geoffrey.pdf https://research.library.mun.ca/7056/3/Whiteway_Geoffrey.pdf |
genre |
Snow crab |
genre_facet |
Snow crab |
op_relation |
https://research.library.mun.ca/7056/1/Whiteway_Geoffrey.pdf https://research.library.mun.ca/7056/3/Whiteway_Geoffrey.pdf Whiteway, Geoffrey <https://research.library.mun.ca/view/creator_az/Whiteway=3AGeoffrey=3A=3A.html> (2003) The identification of aerosolized snow crab protein by electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry. Masters thesis, Memorial University of Newfoundland. |
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thesis_license |
_version_ |
1778533449643065344 |