Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing
An extraction process for the recovery of proteolytic enzymes from the digestive tract of cod (Gadus morha) is described. Processing variables were optimized by employing a rotatable experimental design with computer graphics-assisted response surface methodology (RSM). A simple extraction procedure...
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Memorial University of Newfoundland
1993
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Online Access: | https://research.library.mun.ca/4079/ https://research.library.mun.ca/4079/1/Han_Xiao-Qing.pdf https://research.library.mun.ca/4079/3/Han_Xiao-Qing.pdf |
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ftmemorialuniv:oai:research.library.mun.ca:4079 2023-10-01T03:54:33+02:00 Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing Han, Xiao-Qing 1993 application/pdf https://research.library.mun.ca/4079/ https://research.library.mun.ca/4079/1/Han_Xiao-Qing.pdf https://research.library.mun.ca/4079/3/Han_Xiao-Qing.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/4079/1/Han_Xiao-Qing.pdf https://research.library.mun.ca/4079/3/Han_Xiao-Qing.pdf Han, Xiao-Qing <https://research.library.mun.ca/view/creator_az/Han=3AXiao-Qing=3A=3A.html> (1993) Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing. Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 1993 ftmemorialuniv 2023-09-03T06:44:51Z An extraction process for the recovery of proteolytic enzymes from the digestive tract of cod (Gadus morha) is described. Processing variables were optimized by employing a rotatable experimental design with computer graphics-assisted response surface methodology (RSM). A simple extraction procedure was developed which effectively isolated both acid and alkaline proteases from cod viscera with recovery yields of 52% and 30%, respectively. Further purification and characterization revealed that the crude acid proteases consisted of three types of gastric enzymes designated as acid proteases A, B, and C. Acid protease B was classified as fish pepsin II. Acid proteases A and C possessed properties similar to chymosin and gastricsin, respectively. The partially purified alkaline proteases possessed properties of trypsin-like enzymes and acted on N-benzoyl-L-tyrosine ethyl ester (BTEE), a synthetic substrate for chymotrypsin. -- Utilization of the crude isolated proteases was tested in milk-clotting for cheese making, as well as preparation of protein hydrolysates from under-utilized fish species. Cod pepsin was capable of clotting milk efficiently at low temperatures, which shows its potential use in cold renneting of milk. Capelin protein hydrolysate with a recovery yield of 55.8% was obtained when crude cod gastric proteases were used over a 4 h hydrolysis period at ambient temperatures. Thesis atlantic cod Gadus morhua Memorial University of Newfoundland: Research Repository |
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Memorial University of Newfoundland: Research Repository |
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English |
description |
An extraction process for the recovery of proteolytic enzymes from the digestive tract of cod (Gadus morha) is described. Processing variables were optimized by employing a rotatable experimental design with computer graphics-assisted response surface methodology (RSM). A simple extraction procedure was developed which effectively isolated both acid and alkaline proteases from cod viscera with recovery yields of 52% and 30%, respectively. Further purification and characterization revealed that the crude acid proteases consisted of three types of gastric enzymes designated as acid proteases A, B, and C. Acid protease B was classified as fish pepsin II. Acid proteases A and C possessed properties similar to chymosin and gastricsin, respectively. The partially purified alkaline proteases possessed properties of trypsin-like enzymes and acted on N-benzoyl-L-tyrosine ethyl ester (BTEE), a synthetic substrate for chymotrypsin. -- Utilization of the crude isolated proteases was tested in milk-clotting for cheese making, as well as preparation of protein hydrolysates from under-utilized fish species. Cod pepsin was capable of clotting milk efficiently at low temperatures, which shows its potential use in cold renneting of milk. Capelin protein hydrolysate with a recovery yield of 55.8% was obtained when crude cod gastric proteases were used over a 4 h hydrolysis period at ambient temperatures. |
format |
Thesis |
author |
Han, Xiao-Qing |
spellingShingle |
Han, Xiao-Qing Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing |
author_facet |
Han, Xiao-Qing |
author_sort |
Han, Xiao-Qing |
title |
Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing |
title_short |
Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing |
title_full |
Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing |
title_fullStr |
Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing |
title_full_unstemmed |
Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing |
title_sort |
recovery of digestive enzymes from atlantic cod (gadus morhua) and their utilization in food processing |
publisher |
Memorial University of Newfoundland |
publishDate |
1993 |
url |
https://research.library.mun.ca/4079/ https://research.library.mun.ca/4079/1/Han_Xiao-Qing.pdf https://research.library.mun.ca/4079/3/Han_Xiao-Qing.pdf |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://research.library.mun.ca/4079/1/Han_Xiao-Qing.pdf https://research.library.mun.ca/4079/3/Han_Xiao-Qing.pdf Han, Xiao-Qing <https://research.library.mun.ca/view/creator_az/Han=3AXiao-Qing=3A=3A.html> (1993) Recovery of digestive enzymes from Atlantic cod (Gadus morhua) and their utilization in food processing. Masters thesis, Memorial University of Newfoundland. |
op_rights |
thesis_license |
_version_ |
1778522308621631488 |