Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques
Background: Daily and seasonal changes in temperature are challenges that fish within aquaculture settings cannot completely avoid, and are known to elicit complex organismal and cellular stress responses. We conducted a large-scale gene discovery and transcript expression study in order to better u...
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ftmemorialuniv:oai:research.library.mun.ca:361 2024-09-09T19:29:47+00:00 Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques Hori, Tiago S. Gamperl, A. Kurt Afonso, Luis OB Johnson, Stewart C. Hubert, Sophie Kimball, Jennifer Bowman, Sharen Rise, Matthew L. 2010-01-28 application/pdf https://research.library.mun.ca/361/ https://research.library.mun.ca/361/1/heat_shock_responsive.pdf https://research.library.mun.ca/361/3/heat_shock_responsive.pdf http://www.biomedcentral.com/1471-2164/11/72# en eng BioMed Central Ltd. https://research.library.mun.ca/361/1/heat_shock_responsive.pdf https://research.library.mun.ca/361/3/heat_shock_responsive.pdf Hori, Tiago S. <https://research.library.mun.ca/view/creator_az/Hori=3ATiago_S=2E_=3A=3A.html> and Gamperl, A. Kurt <https://research.library.mun.ca/view/creator_az/Gamperl=3AA=2E_Kurt=3A=3A.html> and Afonso, Luis OB <https://research.library.mun.ca/view/creator_az/Afonso=3ALuis_OB=3A=3A.html> and Johnson, Stewart C. <https://research.library.mun.ca/view/creator_az/Johnson=3AStewart_C=2E_=3A=3A.html> and Hubert, Sophie <https://research.library.mun.ca/view/creator_az/Hubert=3ASophie=3A=3A.html> and Kimball, Jennifer <https://research.library.mun.ca/view/creator_az/Kimball=3AJennifer=3A=3A.html> and Bowman, Sharen <https://research.library.mun.ca/view/creator_az/Bowman=3ASharen=3A=3A.html> and Rise, Matthew L. <https://research.library.mun.ca/view/creator_az/Rise=3AMatthew_L=2E_=3A=3A.html> (2010) Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques. BMC Genomics, 11 (72). pp. 1-22. ISSN 1471-2164 cc_by QH301 Biology QH426 Genetics Article PeerReviewed 2010 ftmemorialuniv 2024-07-10T03:16:00Z Background: Daily and seasonal changes in temperature are challenges that fish within aquaculture settings cannot completely avoid, and are known to elicit complex organismal and cellular stress responses. We conducted a large-scale gene discovery and transcript expression study in order to better understand the genes that are potentially involved in the physiological and cellular aspects of stress caused by heat-shock. We used suppression subtractive hybridization (SSH) cDNA library construction and characterization to identify transcripts that were dysregulated by heat-shock in liver, skeletal muscle and head kidney of Atlantic cod. These tissues were selected due to their roles in metabolic regulation, locomotion and growth, and immune function, respectively. Fish were exposed for 3 hours to an 8°C elevation in temperature, and then allowed to recover for 24 hours at the original temperature (i.e. 10°C). Tissue samples obtained before heat-shock (BHS), at the cessation of heat-shock (CS), and 3, 12, and 24 hours after the cessation of heat-shock (ACS), were used for reciprocal SSH library construction and quantitative reverse transcription - polymerase chain reaction (QPCR) analysis of gene expression using samples from a group that was transferred but not heat-shocked (CT) as controls.Results: We sequenced and characterized 4394 ESTs (1524 from liver, 1451 from head kidney and 1419 from skeletal muscle) from three "forward subtracted" libraries (enriched for genes up-regulated by heat-shock) and 1586 from the liver "reverse subtracted" library (enriched for genes down-regulated by heat-shock), for a total of 5980 ESTs. Several cDNAs encoding putative chaperones belonging to the heat-shock protein (HSP) family were found in these libraries, and "protein folding" was among the gene ontology (GO) terms with the highest proportion in the libraries. QPCR analysis of HSP90α and HSP70-1 (synonym: HSPA1A) mRNA expression showed significant up-regulation in all three tissues studied. These transcripts were more than ... Article in Journal/Newspaper atlantic cod Gadus morhua Memorial University of Newfoundland: Research Repository |
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Open Polar |
collection |
Memorial University of Newfoundland: Research Repository |
op_collection_id |
ftmemorialuniv |
language |
English |
topic |
QH301 Biology QH426 Genetics |
spellingShingle |
QH301 Biology QH426 Genetics Hori, Tiago S. Gamperl, A. Kurt Afonso, Luis OB Johnson, Stewart C. Hubert, Sophie Kimball, Jennifer Bowman, Sharen Rise, Matthew L. Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
topic_facet |
QH301 Biology QH426 Genetics |
description |
Background: Daily and seasonal changes in temperature are challenges that fish within aquaculture settings cannot completely avoid, and are known to elicit complex organismal and cellular stress responses. We conducted a large-scale gene discovery and transcript expression study in order to better understand the genes that are potentially involved in the physiological and cellular aspects of stress caused by heat-shock. We used suppression subtractive hybridization (SSH) cDNA library construction and characterization to identify transcripts that were dysregulated by heat-shock in liver, skeletal muscle and head kidney of Atlantic cod. These tissues were selected due to their roles in metabolic regulation, locomotion and growth, and immune function, respectively. Fish were exposed for 3 hours to an 8°C elevation in temperature, and then allowed to recover for 24 hours at the original temperature (i.e. 10°C). Tissue samples obtained before heat-shock (BHS), at the cessation of heat-shock (CS), and 3, 12, and 24 hours after the cessation of heat-shock (ACS), were used for reciprocal SSH library construction and quantitative reverse transcription - polymerase chain reaction (QPCR) analysis of gene expression using samples from a group that was transferred but not heat-shocked (CT) as controls.Results: We sequenced and characterized 4394 ESTs (1524 from liver, 1451 from head kidney and 1419 from skeletal muscle) from three "forward subtracted" libraries (enriched for genes up-regulated by heat-shock) and 1586 from the liver "reverse subtracted" library (enriched for genes down-regulated by heat-shock), for a total of 5980 ESTs. Several cDNAs encoding putative chaperones belonging to the heat-shock protein (HSP) family were found in these libraries, and "protein folding" was among the gene ontology (GO) terms with the highest proportion in the libraries. QPCR analysis of HSP90α and HSP70-1 (synonym: HSPA1A) mRNA expression showed significant up-regulation in all three tissues studied. These transcripts were more than ... |
format |
Article in Journal/Newspaper |
author |
Hori, Tiago S. Gamperl, A. Kurt Afonso, Luis OB Johnson, Stewart C. Hubert, Sophie Kimball, Jennifer Bowman, Sharen Rise, Matthew L. |
author_facet |
Hori, Tiago S. Gamperl, A. Kurt Afonso, Luis OB Johnson, Stewart C. Hubert, Sophie Kimball, Jennifer Bowman, Sharen Rise, Matthew L. |
author_sort |
Hori, Tiago S. |
title |
Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
title_short |
Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
title_full |
Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
title_fullStr |
Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
title_full_unstemmed |
Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
title_sort |
heat-shock responsive genes identified and validated in atlantic cod (gadus morhua) liver, head kidney and skeletal muscle using genomic techniques |
publisher |
BioMed Central Ltd. |
publishDate |
2010 |
url |
https://research.library.mun.ca/361/ https://research.library.mun.ca/361/1/heat_shock_responsive.pdf https://research.library.mun.ca/361/3/heat_shock_responsive.pdf http://www.biomedcentral.com/1471-2164/11/72# |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
https://research.library.mun.ca/361/1/heat_shock_responsive.pdf https://research.library.mun.ca/361/3/heat_shock_responsive.pdf Hori, Tiago S. <https://research.library.mun.ca/view/creator_az/Hori=3ATiago_S=2E_=3A=3A.html> and Gamperl, A. Kurt <https://research.library.mun.ca/view/creator_az/Gamperl=3AA=2E_Kurt=3A=3A.html> and Afonso, Luis OB <https://research.library.mun.ca/view/creator_az/Afonso=3ALuis_OB=3A=3A.html> and Johnson, Stewart C. <https://research.library.mun.ca/view/creator_az/Johnson=3AStewart_C=2E_=3A=3A.html> and Hubert, Sophie <https://research.library.mun.ca/view/creator_az/Hubert=3ASophie=3A=3A.html> and Kimball, Jennifer <https://research.library.mun.ca/view/creator_az/Kimball=3AJennifer=3A=3A.html> and Bowman, Sharen <https://research.library.mun.ca/view/creator_az/Bowman=3ASharen=3A=3A.html> and Rise, Matthew L. <https://research.library.mun.ca/view/creator_az/Rise=3AMatthew_L=2E_=3A=3A.html> (2010) Heat-shock responsive genes identified and validated in Atlantic cod (Gadus morhua) liver, head kidney and skeletal muscle using genomic techniques. BMC Genomics, 11 (72). pp. 1-22. ISSN 1471-2164 |
op_rights |
cc_by |
_version_ |
1809898882493054976 |