Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica)
Dispersal and philopatry play key roles in population structure and gene flow for vagile species such as seabirds. Seabirds, including the Atlantic puffin (Fratercula arctica), are known to have strong breeding philopatry, but due to challenges of studying pre-breeding individuals, little is known a...
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Memorial University of Newfoundland
2023
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ftmemorialuniv:oai:research.library.mun.ca:16065 2023-11-12T04:14:24+01:00 Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) Wilson, Amy Catherine 2023-08 application/pdf https://research.library.mun.ca/16065/ https://research.library.mun.ca/16065/1/converted.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/16065/1/converted.pdf Wilson, Amy Catherine <https://research.library.mun.ca/view/creator_az/Wilson=3AAmy_Catherine=3A=3A.html> (2023) Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica). Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2023 ftmemorialuniv 2023-10-28T23:12:35Z Dispersal and philopatry play key roles in population structure and gene flow for vagile species such as seabirds. Seabirds, including the Atlantic puffin (Fratercula arctica), are known to have strong breeding philopatry, but due to challenges of studying pre-breeding individuals, little is known about their natal philopatry or dispersal. My thesis investigated Atlantic puffins on Gull Island, Newfoundland and Labrador, using restriction site associated DNA (RAD) sequencing to assess: if fine-scale genetic population structure is detectable at the colony level, if there are differences in dispersal between the sexes, and the level of relatedness among individuals. I predicted that natal philopatry should generate genetic population structure at the colony level. Blood from Atlantic puffins in eight plots was collected, extracted, and sent for sequencing. Genetic admixture, principal component analysis (PCA), and Mantel tests were used to assess structure. The admixture and PCA found no evidence of genetic differentiation between plots or sex and the Mantel test found no significant correlations between genetic and geographic distance. I found little evidence of population structure within the colony, which suggests there may be natal dispersal in this species. Natal dispersal may be an important life history trait for maintaining gene flow in species. Thesis Atlantic puffin fratercula Fratercula arctica Newfoundland Memorial University of Newfoundland: Research Repository |
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Memorial University of Newfoundland: Research Repository |
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English |
description |
Dispersal and philopatry play key roles in population structure and gene flow for vagile species such as seabirds. Seabirds, including the Atlantic puffin (Fratercula arctica), are known to have strong breeding philopatry, but due to challenges of studying pre-breeding individuals, little is known about their natal philopatry or dispersal. My thesis investigated Atlantic puffins on Gull Island, Newfoundland and Labrador, using restriction site associated DNA (RAD) sequencing to assess: if fine-scale genetic population structure is detectable at the colony level, if there are differences in dispersal between the sexes, and the level of relatedness among individuals. I predicted that natal philopatry should generate genetic population structure at the colony level. Blood from Atlantic puffins in eight plots was collected, extracted, and sent for sequencing. Genetic admixture, principal component analysis (PCA), and Mantel tests were used to assess structure. The admixture and PCA found no evidence of genetic differentiation between plots or sex and the Mantel test found no significant correlations between genetic and geographic distance. I found little evidence of population structure within the colony, which suggests there may be natal dispersal in this species. Natal dispersal may be an important life history trait for maintaining gene flow in species. |
format |
Thesis |
author |
Wilson, Amy Catherine |
spellingShingle |
Wilson, Amy Catherine Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) |
author_facet |
Wilson, Amy Catherine |
author_sort |
Wilson, Amy Catherine |
title |
Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) |
title_short |
Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) |
title_full |
Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) |
title_fullStr |
Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) |
title_full_unstemmed |
Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica) |
title_sort |
assessing fine-scale population structure using rad sequencing in a philopatric seabird, the atlantic puffin (fratercula arctica) |
publisher |
Memorial University of Newfoundland |
publishDate |
2023 |
url |
https://research.library.mun.ca/16065/ https://research.library.mun.ca/16065/1/converted.pdf |
genre |
Atlantic puffin fratercula Fratercula arctica Newfoundland |
genre_facet |
Atlantic puffin fratercula Fratercula arctica Newfoundland |
op_relation |
https://research.library.mun.ca/16065/1/converted.pdf Wilson, Amy Catherine <https://research.library.mun.ca/view/creator_az/Wilson=3AAmy_Catherine=3A=3A.html> (2023) Assessing fine-scale population structure using RAD sequencing in a philopatric seabird, the Atlantic puffin (Fratercula arctica). Masters thesis, Memorial University of Newfoundland. |
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