Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids
This thesis involved a study of the effect of North American (NA) farmed, European origin (EO) farmed, wild, and related hybrid cross types on the innate antiviral-like immune response in Atlantic salmon (Salmo salar). Since the 1970s, millions of farmed salmon have escaped causing concern about how...
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Memorial University of Newfoundland
2022
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ftmemorialuniv:oai:research.library.mun.ca:15720 2023-10-01T03:54:44+02:00 Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids Dove, Sindy 2022-12 application/pdf https://research.library.mun.ca/15720/ https://research.library.mun.ca/15720/3/converted.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/15720/3/converted.pdf Dove, Sindy <https://research.library.mun.ca/view/creator_az/Dove=3ASindy=3A=3A.html> (2022) Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids. Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2022 ftmemorialuniv 2023-09-03T06:50:24Z This thesis involved a study of the effect of North American (NA) farmed, European origin (EO) farmed, wild, and related hybrid cross types on the innate antiviral-like immune response in Atlantic salmon (Salmo salar). Since the 1970s, millions of farmed salmon have escaped causing concern about how interbreeding with wild salmon may affect wild populations. Farmed and wild salmon differ genetically, yet the immune responses of farmed-wild hybrids are not well understood. In Newfoundland, permission has been granted to start farming an EO strain in addition to the current NA strain from the Saint John River. As these strains differ genetically and geographically, there is concern about potential differences in immune responses. Hence, I used real-time quantitative polymerase chain reaction (qPCR) with head kidney templates to measure expression of seventeen antiviral and other immune-relevant biomarker genes (i.e., dhx58, gig1a, helz2, ifna, ifng, irf1a, irf7b, isg15a, mxb, pgds, rsad2a, rsad2b, stat1a, stat1c, tlr3, tlr7, and 5loxb) to assess the innate antiviral-like immune responses [i.e. to intraperitoneal (IP) injection with polyriboinosinic polyribocytidylic (pIC)] of six different Atlantic salmon cross types including pure crosses (i.e. NA, EO, and local Northeast Placentia River Wild) and three related hybrid crosses. Cross type abbreviations are as follows: pure Wild; pure European farmed (Farm.EO); pure North American farmed (Farm.NA); wild female crossed with Farm.EO male (Hyb.EO w♀); Farm.EO female crossed with wild male (Hyb.EO F♀); and Farm.NA female crossed with wild male (Hyb.NA F♀). At a basal transcript expression level, only one gene (pgds) showed significant cross type differences, where Hyb.NA F♀ had significantly higher expression than the Wild and Hyb.EO F♀. After pIC injection, fifteen of the seventeen target genes (i.e. all except pgds and 5loxb) were significantly upregulated. In addition, there were significant cross type differences in transcript expression of ifna, isg15a, rsad2a, ... Thesis Atlantic salmon Newfoundland Salmo salar Memorial University of Newfoundland: Research Repository |
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Memorial University of Newfoundland: Research Repository |
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This thesis involved a study of the effect of North American (NA) farmed, European origin (EO) farmed, wild, and related hybrid cross types on the innate antiviral-like immune response in Atlantic salmon (Salmo salar). Since the 1970s, millions of farmed salmon have escaped causing concern about how interbreeding with wild salmon may affect wild populations. Farmed and wild salmon differ genetically, yet the immune responses of farmed-wild hybrids are not well understood. In Newfoundland, permission has been granted to start farming an EO strain in addition to the current NA strain from the Saint John River. As these strains differ genetically and geographically, there is concern about potential differences in immune responses. Hence, I used real-time quantitative polymerase chain reaction (qPCR) with head kidney templates to measure expression of seventeen antiviral and other immune-relevant biomarker genes (i.e., dhx58, gig1a, helz2, ifna, ifng, irf1a, irf7b, isg15a, mxb, pgds, rsad2a, rsad2b, stat1a, stat1c, tlr3, tlr7, and 5loxb) to assess the innate antiviral-like immune responses [i.e. to intraperitoneal (IP) injection with polyriboinosinic polyribocytidylic (pIC)] of six different Atlantic salmon cross types including pure crosses (i.e. NA, EO, and local Northeast Placentia River Wild) and three related hybrid crosses. Cross type abbreviations are as follows: pure Wild; pure European farmed (Farm.EO); pure North American farmed (Farm.NA); wild female crossed with Farm.EO male (Hyb.EO w♀); Farm.EO female crossed with wild male (Hyb.EO F♀); and Farm.NA female crossed with wild male (Hyb.NA F♀). At a basal transcript expression level, only one gene (pgds) showed significant cross type differences, where Hyb.NA F♀ had significantly higher expression than the Wild and Hyb.EO F♀. After pIC injection, fifteen of the seventeen target genes (i.e. all except pgds and 5loxb) were significantly upregulated. In addition, there were significant cross type differences in transcript expression of ifna, isg15a, rsad2a, ... |
format |
Thesis |
author |
Dove, Sindy |
spellingShingle |
Dove, Sindy Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids |
author_facet |
Dove, Sindy |
author_sort |
Dove, Sindy |
title |
Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids |
title_short |
Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids |
title_full |
Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids |
title_fullStr |
Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids |
title_full_unstemmed |
Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids |
title_sort |
innate antiviral-like immune responses differ among divergent european and north american farmed and wild atlantic salmon (salmo salar) and their hybrids |
publisher |
Memorial University of Newfoundland |
publishDate |
2022 |
url |
https://research.library.mun.ca/15720/ https://research.library.mun.ca/15720/3/converted.pdf |
genre |
Atlantic salmon Newfoundland Salmo salar |
genre_facet |
Atlantic salmon Newfoundland Salmo salar |
op_relation |
https://research.library.mun.ca/15720/3/converted.pdf Dove, Sindy <https://research.library.mun.ca/view/creator_az/Dove=3ASindy=3A=3A.html> (2022) Innate antiviral-like immune responses differ among divergent European and North American farmed and wild Atlantic salmon (Salmo salar) and their hybrids. Masters thesis, Memorial University of Newfoundland. |
op_rights |
thesis_license |
_version_ |
1778522630677069824 |