Macrophage migration inhibitory factor may play a protective role in osteoarthritis
Background Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular...
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ftmemorialuniv:oai:research.library.mun.ca:15377 2023-10-01T03:57:39+02:00 Macrophage migration inhibitory factor may play a protective role in osteoarthritis Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju 2021-02-20 application/pdf https://research.library.mun.ca/15377/ https://research.library.mun.ca/15377/1/s13075-021-02442-w.pdf https://doi.org/10.1186/s13075-021-02442-w en eng BioMed Central (BMC) https://research.library.mun.ca/15377/1/s13075-021-02442-w.pdf Liu, Ming <https://research.library.mun.ca/view/creator_az/Liu=3AMing=3A=3A.html> and Xie, Zikun <https://research.library.mun.ca/view/creator_az/Xie=3AZikun=3A=3A.html> and Sun, Guang <https://research.library.mun.ca/view/creator_az/Sun=3AGuang=3A=3A.html> and Chen, Liujun <https://research.library.mun.ca/view/creator_az/Chen=3ALiujun=3A=3A.html> and Qi, Dake <https://research.library.mun.ca/view/creator_az/Qi=3ADake=3A=3A.html> and Zhang, Hongwei <https://research.library.mun.ca/view/creator_az/Zhang=3AHongwei=3A=3A.html> and Xiong, Jieying <https://research.library.mun.ca/view/creator_az/Xiong=3AJieying=3A=3A.html> and Furey, Andrew <https://research.library.mun.ca/view/creator_az/Furey=3AAndrew=3A=3A.html> and Rahman, Proton <https://research.library.mun.ca/view/creator_az/Rahman=3AProton=3A=3A.html> and Lei, Guanghua <https://research.library.mun.ca/view/creator_az/Lei=3AGuanghua=3A=3A.html> and Zhai, Guangju <https://research.library.mun.ca/view/creator_az/Zhai=3AGuangju=3A=3A.html> (2021) Macrophage migration inhibitory factor may play a protective role in osteoarthritis. Arthritis Research & Therapy, 23. ISSN 1478-6362 cc_by_nc Article PeerReviewed 2021 ftmemorialuniv https://doi.org/10.1186/s13075-021-02442-w 2023-09-03T06:50:12Z Background Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular mechanisms. Macrophage migration inhibitory factor (MIF) is a procytokine that mediates pleiotropic inflammatory effects in inflammatory diseases such as rheumatoid arthritis (RA) and ankylosing spondylitis (AS). However, data on the role of MIF in OA is limited with conflicting results. We undertook this study to investigate the role of MIF in OA by examining MIF genotype, mRNA expression, and protein levels in the Newfoundland Osteoarthritis Study. Methods One hundred nineteen end-stage knee/hip OA patients, 16 RA patients, and 113 healthy controls were included in the study. Two polymorphisms in the MIF gene, rs755622, and -794 CATT5-8, were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR followed by automated capillary electrophoresis, respectively. MIF mRNA levels in articular cartilage and subchondral bone were measured by quantitative polymerase chain reaction. Plasma concentrations of MIF, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured by enzyme-linked immunosorbent assay. Results rs755622 and -794 CATT5-8 genotypes were not associated with MIF mRNA or protein levels or OA (all p ≥ 0.19). MIF mRNA level in cartilage was lower in OA patients than in controls (p = 0.028) and RA patients (p = 0.004), while the levels in bone were comparable between OA patients and controls (p = 0.165). MIF protein level in plasma was lower in OA patients than in controls (p = 3.01 × 10−10), while the levels of TNF-α, IL-6 and IL-1β in plasma were all significantly higher in OA patients than in controls (all p ≤ 0.0007). Multivariable logistic regression showed lower MIF and higher IL-1β protein levels in plasma were ... Article in Journal/Newspaper Newfoundland Memorial University of Newfoundland: Research Repository Arthritis Research & Therapy 23 1 |
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Memorial University of Newfoundland: Research Repository |
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ftmemorialuniv |
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English |
description |
Background Osteoarthritis (OA) is the most prevalent form of arthritis and the major cause of disability and overall diminution of quality of life in the elderly population. Currently there is no cure for OA, partly due to the large gaps in our understanding of its underlying molecular and cellular mechanisms. Macrophage migration inhibitory factor (MIF) is a procytokine that mediates pleiotropic inflammatory effects in inflammatory diseases such as rheumatoid arthritis (RA) and ankylosing spondylitis (AS). However, data on the role of MIF in OA is limited with conflicting results. We undertook this study to investigate the role of MIF in OA by examining MIF genotype, mRNA expression, and protein levels in the Newfoundland Osteoarthritis Study. Methods One hundred nineteen end-stage knee/hip OA patients, 16 RA patients, and 113 healthy controls were included in the study. Two polymorphisms in the MIF gene, rs755622, and -794 CATT5-8, were genotyped using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) and PCR followed by automated capillary electrophoresis, respectively. MIF mRNA levels in articular cartilage and subchondral bone were measured by quantitative polymerase chain reaction. Plasma concentrations of MIF, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) were measured by enzyme-linked immunosorbent assay. Results rs755622 and -794 CATT5-8 genotypes were not associated with MIF mRNA or protein levels or OA (all p ≥ 0.19). MIF mRNA level in cartilage was lower in OA patients than in controls (p = 0.028) and RA patients (p = 0.004), while the levels in bone were comparable between OA patients and controls (p = 0.165). MIF protein level in plasma was lower in OA patients than in controls (p = 3.01 × 10−10), while the levels of TNF-α, IL-6 and IL-1β in plasma were all significantly higher in OA patients than in controls (all p ≤ 0.0007). Multivariable logistic regression showed lower MIF and higher IL-1β protein levels in plasma were ... |
format |
Article in Journal/Newspaper |
author |
Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju |
spellingShingle |
Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
author_facet |
Liu, Ming Xie, Zikun Sun, Guang Chen, Liujun Qi, Dake Zhang, Hongwei Xiong, Jieying Furey, Andrew Rahman, Proton Lei, Guanghua Zhai, Guangju |
author_sort |
Liu, Ming |
title |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_short |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_full |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_fullStr |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_full_unstemmed |
Macrophage migration inhibitory factor may play a protective role in osteoarthritis |
title_sort |
macrophage migration inhibitory factor may play a protective role in osteoarthritis |
publisher |
BioMed Central (BMC) |
publishDate |
2021 |
url |
https://research.library.mun.ca/15377/ https://research.library.mun.ca/15377/1/s13075-021-02442-w.pdf https://doi.org/10.1186/s13075-021-02442-w |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
https://research.library.mun.ca/15377/1/s13075-021-02442-w.pdf Liu, Ming <https://research.library.mun.ca/view/creator_az/Liu=3AMing=3A=3A.html> and Xie, Zikun <https://research.library.mun.ca/view/creator_az/Xie=3AZikun=3A=3A.html> and Sun, Guang <https://research.library.mun.ca/view/creator_az/Sun=3AGuang=3A=3A.html> and Chen, Liujun <https://research.library.mun.ca/view/creator_az/Chen=3ALiujun=3A=3A.html> and Qi, Dake <https://research.library.mun.ca/view/creator_az/Qi=3ADake=3A=3A.html> and Zhang, Hongwei <https://research.library.mun.ca/view/creator_az/Zhang=3AHongwei=3A=3A.html> and Xiong, Jieying <https://research.library.mun.ca/view/creator_az/Xiong=3AJieying=3A=3A.html> and Furey, Andrew <https://research.library.mun.ca/view/creator_az/Furey=3AAndrew=3A=3A.html> and Rahman, Proton <https://research.library.mun.ca/view/creator_az/Rahman=3AProton=3A=3A.html> and Lei, Guanghua <https://research.library.mun.ca/view/creator_az/Lei=3AGuanghua=3A=3A.html> and Zhai, Guangju <https://research.library.mun.ca/view/creator_az/Zhai=3AGuangju=3A=3A.html> (2021) Macrophage migration inhibitory factor may play a protective role in osteoarthritis. Arthritis Research & Therapy, 23. ISSN 1478-6362 |
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cc_by_nc |
op_doi |
https://doi.org/10.1186/s13075-021-02442-w |
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