Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea
Coral bacterial associates can play important functional roles for the holobiont, such as nitrogen cycling, nutrient processing, and supporting immunity. While bacteria found within the microbiome of corals may benefit the host, they can also be linked to pathogenesis. In the deep-sea, cold-water co...
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Online Access: | https://research.library.mun.ca/13664/ https://research.library.mun.ca/13664/1/fmars-05-00378.pdf https://doi.org/10.3389/fmars.2018.00378 |
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ftmemorialuniv:oai:research.library.mun.ca:13664 2023-10-01T03:58:45+02:00 Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea Weiler, Bradley A. Verhoeven, Joost T. P. Dufour, Suzanne C. 2018-10-18 application/pdf https://research.library.mun.ca/13664/ https://research.library.mun.ca/13664/1/fmars-05-00378.pdf https://doi.org/10.3389/fmars.2018.00378 en eng Frontiers Media https://research.library.mun.ca/13664/1/fmars-05-00378.pdf Weiler, Bradley A. <https://research.library.mun.ca/view/creator_az/Weiler=3ABradley_A=2E=3A=3A.html> and Verhoeven, Joost T. P. <https://research.library.mun.ca/view/creator_az/Verhoeven=3AJoost_T=2E_P=2E=3A=3A.html> and Dufour, Suzanne C. <https://research.library.mun.ca/view/creator_az/Dufour=3ASuzanne_C=2E=3A=3A.html> (2018) Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea. Frontiers in Marine Science, 5. ISSN 2296-7745 cc_by_nc Article PeerReviewed 2018 ftmemorialuniv https://doi.org/10.3389/fmars.2018.00378 2023-09-03T06:49:23Z Coral bacterial associates can play important functional roles for the holobiont, such as nitrogen cycling, nutrient processing, and supporting immunity. While bacteria found within the microbiome of corals may benefit the host, they can also be linked to pathogenesis. In the deep-sea, cold-water corals, like their warm shallow-water counterparts, host bacterial communities, but have received little attention due to logistical constraints in sampling. In particular, bacteria associated with surficial mucus of cold-water corals have not yet been investigated. Here, tissue and mucus samples of Paragorgia arborea were collected from three submarine canyons along the continental slope of the Gulf of Maine. Bacterial DNA was extracted from tissue and mucus samples and sequencing of the V6–V8 hypervariable region of the 16S rRNA gene was performed using Illumina MiSeq. The bacterial communities associated with P. arborea compartments (tissue and mucus) and sampling locations (canyon) differed significantly in composition. Proteobacteria, Tenericutes, and Spirochaetes were the dominant phyla across the majority of coral tissue samples, with Gammaproteobacteria and Alphaproteobacteria identified as the largest Proteobacteria contributors across all samples. Operational taxonomic units (OTUs) belonging to the taxa Spirochaeta, Mycoplasma, Flavobacteriaceae, Terasakiellaceae, Campylobacterales, and Rickettsiales were identified as biomarkers (bacterial taxa significantly more abundant in a specific coral microhabitat) of P. arborea tissues, while Paracoccus was a biomarker of P. arborea mucus. Many of the recovered biomarker taxa may be involved in nitrogen cycling. Representatives from several bacterial families (Vibrionaceae, Campylobacteraceae, Rhodobacteraceae, Flavobacteriaceae, and Burkholderiaceae) previously reported in diseased scleractinians, were present in P. arborea as rare bacterial taxa. Characterizing the bacterial associates present in visibly healthy coral colonies provides a benchmark of dominant and ... Article in Journal/Newspaper Paragorgia arborea Memorial University of Newfoundland: Research Repository Frontiers in Marine Science 5 |
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Open Polar |
collection |
Memorial University of Newfoundland: Research Repository |
op_collection_id |
ftmemorialuniv |
language |
English |
description |
Coral bacterial associates can play important functional roles for the holobiont, such as nitrogen cycling, nutrient processing, and supporting immunity. While bacteria found within the microbiome of corals may benefit the host, they can also be linked to pathogenesis. In the deep-sea, cold-water corals, like their warm shallow-water counterparts, host bacterial communities, but have received little attention due to logistical constraints in sampling. In particular, bacteria associated with surficial mucus of cold-water corals have not yet been investigated. Here, tissue and mucus samples of Paragorgia arborea were collected from three submarine canyons along the continental slope of the Gulf of Maine. Bacterial DNA was extracted from tissue and mucus samples and sequencing of the V6–V8 hypervariable region of the 16S rRNA gene was performed using Illumina MiSeq. The bacterial communities associated with P. arborea compartments (tissue and mucus) and sampling locations (canyon) differed significantly in composition. Proteobacteria, Tenericutes, and Spirochaetes were the dominant phyla across the majority of coral tissue samples, with Gammaproteobacteria and Alphaproteobacteria identified as the largest Proteobacteria contributors across all samples. Operational taxonomic units (OTUs) belonging to the taxa Spirochaeta, Mycoplasma, Flavobacteriaceae, Terasakiellaceae, Campylobacterales, and Rickettsiales were identified as biomarkers (bacterial taxa significantly more abundant in a specific coral microhabitat) of P. arborea tissues, while Paracoccus was a biomarker of P. arborea mucus. Many of the recovered biomarker taxa may be involved in nitrogen cycling. Representatives from several bacterial families (Vibrionaceae, Campylobacteraceae, Rhodobacteraceae, Flavobacteriaceae, and Burkholderiaceae) previously reported in diseased scleractinians, were present in P. arborea as rare bacterial taxa. Characterizing the bacterial associates present in visibly healthy coral colonies provides a benchmark of dominant and ... |
format |
Article in Journal/Newspaper |
author |
Weiler, Bradley A. Verhoeven, Joost T. P. Dufour, Suzanne C. |
spellingShingle |
Weiler, Bradley A. Verhoeven, Joost T. P. Dufour, Suzanne C. Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea |
author_facet |
Weiler, Bradley A. Verhoeven, Joost T. P. Dufour, Suzanne C. |
author_sort |
Weiler, Bradley A. |
title |
Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea |
title_short |
Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea |
title_full |
Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea |
title_fullStr |
Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea |
title_full_unstemmed |
Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea |
title_sort |
bacterial communities in tissues and surficial mucus of the cold-water coral paragorgia arborea |
publisher |
Frontiers Media |
publishDate |
2018 |
url |
https://research.library.mun.ca/13664/ https://research.library.mun.ca/13664/1/fmars-05-00378.pdf https://doi.org/10.3389/fmars.2018.00378 |
genre |
Paragorgia arborea |
genre_facet |
Paragorgia arborea |
op_relation |
https://research.library.mun.ca/13664/1/fmars-05-00378.pdf Weiler, Bradley A. <https://research.library.mun.ca/view/creator_az/Weiler=3ABradley_A=2E=3A=3A.html> and Verhoeven, Joost T. P. <https://research.library.mun.ca/view/creator_az/Verhoeven=3AJoost_T=2E_P=2E=3A=3A.html> and Dufour, Suzanne C. <https://research.library.mun.ca/view/creator_az/Dufour=3ASuzanne_C=2E=3A=3A.html> (2018) Bacterial Communities in Tissues and Surficial Mucus of the Cold-Water Coral Paragorgia arborea. Frontiers in Marine Science, 5. ISSN 2296-7745 |
op_rights |
cc_by_nc |
op_doi |
https://doi.org/10.3389/fmars.2018.00378 |
container_title |
Frontiers in Marine Science |
container_volume |
5 |
_version_ |
1778531894965567488 |