Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada
Antibiotic production is a natural phenomenon employed by microorganisms to control their environment in stress situations. Thus, antibiotic resistance has evolved in parallel to counteract naturally produced antibiotics. On the other hand, excessive use of antibiotics by humans, either for protecti...
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Memorial University of Newfoundland
2017
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ftmemorialuniv:oai:research.library.mun.ca:13130 2023-10-01T03:57:35+02:00 Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada Edet, Emana Ifeoma 2017-10 application/pdf https://research.library.mun.ca/13130/ https://research.library.mun.ca/13130/1/thesis.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/13130/1/thesis.pdf Edet, Emana Ifeoma <https://research.library.mun.ca/view/creator_az/Edet=3AEmana_Ifeoma=3A=3A.html> (2017) Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada. Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2017 ftmemorialuniv 2023-09-03T06:49:08Z Antibiotic production is a natural phenomenon employed by microorganisms to control their environment in stress situations. Thus, antibiotic resistance has evolved in parallel to counteract naturally produced antibiotics. On the other hand, excessive use of antibiotics by humans, either for protecting human or animal health, may induce unnaturally high antibiotic stresses leading to enhanced antibiotic resistance, the so-called acquired resistance. This study is the first to report an initial exploration into the distribution and quantification of antibiotic resistance genes, i.e. resistome, in a mixed-use watershed in Western Newfoundland, along the Humber river. The river was sampled along a gradient of increased human impact, mainly associated with the discharge of municipal wastewaters. The goals of my thesis were to (1) understand the relationship between human impact in the Humber river and the occurrence of antibiotic resistance genes in the environment, and (2) understand the role of the river in mitigating the abundance of these resistance genes, if any role is present. Total DNA was extracted from the river water samples, sequenced by shotgun sequencing on an Illumina Hiseq platform with sequencing data quality controlled and cleaned at the sequencing facility via a QIIME pipeline, followed by identification and quantification of antibiotic resistance markers through a computational pipeline carried out in ShortBRED against a curated Antibiotic Resistance Genes Database (ARDB). About 400 antibiotic resistance genes, of variable abundance, were identified, distributed across the tested systems, an indication of the wide distribution of antibiotic resistance in the environment. This analysis revealed the distribution and abundance of Antibiotic Resistance Genes (ARGs) within the microbial population in the Humber River. Quantitative real-time PCR (qRT-PCR) was used to quantify absolute (copies L⁻¹) and relative abundances (copies /16S rRNA) for TetO, TetM and AdeC genes. Relative abundance of ARGs ... Thesis Newfoundland Memorial University of Newfoundland: Research Repository Canada |
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Memorial University of Newfoundland: Research Repository |
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English |
description |
Antibiotic production is a natural phenomenon employed by microorganisms to control their environment in stress situations. Thus, antibiotic resistance has evolved in parallel to counteract naturally produced antibiotics. On the other hand, excessive use of antibiotics by humans, either for protecting human or animal health, may induce unnaturally high antibiotic stresses leading to enhanced antibiotic resistance, the so-called acquired resistance. This study is the first to report an initial exploration into the distribution and quantification of antibiotic resistance genes, i.e. resistome, in a mixed-use watershed in Western Newfoundland, along the Humber river. The river was sampled along a gradient of increased human impact, mainly associated with the discharge of municipal wastewaters. The goals of my thesis were to (1) understand the relationship between human impact in the Humber river and the occurrence of antibiotic resistance genes in the environment, and (2) understand the role of the river in mitigating the abundance of these resistance genes, if any role is present. Total DNA was extracted from the river water samples, sequenced by shotgun sequencing on an Illumina Hiseq platform with sequencing data quality controlled and cleaned at the sequencing facility via a QIIME pipeline, followed by identification and quantification of antibiotic resistance markers through a computational pipeline carried out in ShortBRED against a curated Antibiotic Resistance Genes Database (ARDB). About 400 antibiotic resistance genes, of variable abundance, were identified, distributed across the tested systems, an indication of the wide distribution of antibiotic resistance in the environment. This analysis revealed the distribution and abundance of Antibiotic Resistance Genes (ARGs) within the microbial population in the Humber River. Quantitative real-time PCR (qRT-PCR) was used to quantify absolute (copies L⁻¹) and relative abundances (copies /16S rRNA) for TetO, TetM and AdeC genes. Relative abundance of ARGs ... |
format |
Thesis |
author |
Edet, Emana Ifeoma |
spellingShingle |
Edet, Emana Ifeoma Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada |
author_facet |
Edet, Emana Ifeoma |
author_sort |
Edet, Emana Ifeoma |
title |
Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada |
title_short |
Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada |
title_full |
Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada |
title_fullStr |
Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada |
title_full_unstemmed |
Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada |
title_sort |
survey of antibiotic resistome in a mixed-use watershed in western newfoundland, canada |
publisher |
Memorial University of Newfoundland |
publishDate |
2017 |
url |
https://research.library.mun.ca/13130/ https://research.library.mun.ca/13130/1/thesis.pdf |
geographic |
Canada |
geographic_facet |
Canada |
genre |
Newfoundland |
genre_facet |
Newfoundland |
op_relation |
https://research.library.mun.ca/13130/1/thesis.pdf Edet, Emana Ifeoma <https://research.library.mun.ca/view/creator_az/Edet=3AEmana_Ifeoma=3A=3A.html> (2017) Survey of antibiotic resistome in a mixed-use watershed in Western Newfoundland, Canada. Masters thesis, Memorial University of Newfoundland. |
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thesis_license |
_version_ |
1778529229849231360 |