Cold adaptation of Atlantic salmon tropomyosin: role of residue '77'

• Main Author: Ige, Tolulope Oluwasomo
• Format: Thesis
• Language: English
• Published: Memorial University of Newfoundland 2012
• Subjects: Atlantic salmon; Salmo salar
• Online Access: https://research.library.mun.ca/10716/; https://research.library.mun.ca/10716/1/Ige_TolulopeO.pdf

Tropomyosin from the fast skeletal (trunk) muscle of Atlantic salmon (Salmo salar) is an alpha-type isoform. It shares ~93% identity with a counterpart from rabbit skeletal muscle (20 amino acid substitutions out of a total of 284) but is less thermally stable. -- An interesting aspect of the small heterogeneity is the replacement of lysine-77 in rabbit with threonine in salmon. In the case of the mammalian protein, the lysine in question is positioned to form ion pairs with aspartate-80 in the next turn of the same helix and also with glutamate 82 in the opposing helix of the coiled coil. Thus, at neutral pH, salmon tropomyosin loses two potential charge-charge (ion pair) interactions. The contribution of residue-77 to the properties of salmon tropomyosin has been investigated by mutating it to the amino acid in rabbit using site directed mutagenesis, followed by circular dichroism, differential scanning calorimetry, affinity chromatography and limited proteolysis. A major finding of this research is that threonine in the 77th position of salmon tropomyosin is destabilizing compared to lysine in the same position. Reducing the number of ion pairs is concluded to be part of the structural means by which tropomyosin, and possibly other rod-shaped proteins, adapt to low temperature. The specific details are outlined below in point form: -- 1. Atlantic salmon fast skeletal muscle alpha-tropomyosin cDNA was mutated to replace the threonine at position 77 with lysine using the QuickChange Lightning site directed mutagenesis kit. The mutation was confirmed by DNA sequencing. -- 2. Mutated and non mutated recombinant tropomyosins were obtained by expression in E. coli BL21 cells and chromatographically isolated. -- 3. The mutant salmon tropomyosin, containing Lys-77, exhibited a faster electrophoretic mobility than the non-mutant (Thr-77), in the presence of the detergent sodium dodecyl sulfate, which is attributable to a difference in detergent binding due to the extra charge. This electrophoretic shift was useful in ...