The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar)
Expression of the type Ill antifreeze protein (AFP) gene from ocean pout (OP5α) was examined in ocean pout and Atlantic salmon transgenic fora truncated version this gene (t-OP5α). Also, Atlantic salmon transgenic for an “all-fish chimeric gene construct” composed of the ocean pout type Ill AFP gene...
Main Author: | |
---|---|
Format: | Thesis |
Language: | English |
Published: |
Memorial University of Newfoundland
2005
|
Subjects: | |
Online Access: | https://research.library.mun.ca/10662/ https://research.library.mun.ca/10662/1/Hobbs_RodneyStephen.pdf |
id |
ftmemorialuniv:oai:research.library.mun.ca:10662 |
---|---|
record_format |
openpolar |
spelling |
ftmemorialuniv:oai:research.library.mun.ca:10662 2023-10-01T03:54:43+02:00 The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) Hobbs, Rodney Stephen 2005 application/pdf https://research.library.mun.ca/10662/ https://research.library.mun.ca/10662/1/Hobbs_RodneyStephen.pdf en eng Memorial University of Newfoundland https://research.library.mun.ca/10662/1/Hobbs_RodneyStephen.pdf Hobbs, Rodney Stephen <https://research.library.mun.ca/view/creator_az/Hobbs=3ARodney_Stephen=3A=3A.html> (2005) The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar). Masters thesis, Memorial University of Newfoundland. thesis_license Thesis NonPeerReviewed 2005 ftmemorialuniv 2023-09-03T06:47:58Z Expression of the type Ill antifreeze protein (AFP) gene from ocean pout (OP5α) was examined in ocean pout and Atlantic salmon transgenic fora truncated version this gene (t-OP5α). Also, Atlantic salmon transgenic for an “all-fish chimeric gene construct” composed of the ocean pout type Ill AFP gene 5' and 3' ends linked to the Chinook salmon growth hormone (GH) gene (EO-1α) was examined for GH mRNA. Northern blot analysis of ocean pout demonstrated that AFP mRNA was detected in most tissues with extremely high levels observed in the liver, and high levels in the stomach and gill. Low AFP mRNA levels were observed in skin, mouth skin, intestine, spleen, and kidney, while barely detectable levels were observed in heart, ovary, brain, and blood cells. No AFP mRNA was observed in muscle. For t-OP5α transgenic Atlantic salmon, AFP mRNA was detected in most tissues with high levels observed in heart, liver, stomach, and brain, low levels observed in mouth skin, intestine, spleen, gill, and muscle, and barely detectable levels observed in kidney, ovary and skin. No AFP mRNA was observed in blood cells. Northern blot analysis of EO-1α transgenic salmon demonstrated that expression was observed only in pituitary and spleen, with moderate expression detected in the pituitary and low expression in the spleen. Reverse transcription polymerase chain reaction (RT-PCR) demonstrated that the OP5α gene is expressed in all tissues of the ocean pout, and all tissues except for blood in both transgenic Atlantic salmon lines (t-OP5α and EO-1α). Blood plasma samples from the three strains of fish were tested for AFP activity by determining the thermal hysteresis (TH) for each. TH measurements from winter acclimated ocean pout were high (approximately 1.36°C) with typical bipyrimidal ice crystal shaping indicative of a high amount of AFP, while t-OP5α transgenic fish showed little(< 0.01°C) TH activity, but yielded hexagonal shaped ice crystals indicative of the presence of AFP. On the other hand, EO-1α transgenics showed no (0°C) ... Thesis Atlantic salmon Salmo salar Memorial University of Newfoundland: Research Repository |
institution |
Open Polar |
collection |
Memorial University of Newfoundland: Research Repository |
op_collection_id |
ftmemorialuniv |
language |
English |
description |
Expression of the type Ill antifreeze protein (AFP) gene from ocean pout (OP5α) was examined in ocean pout and Atlantic salmon transgenic fora truncated version this gene (t-OP5α). Also, Atlantic salmon transgenic for an “all-fish chimeric gene construct” composed of the ocean pout type Ill AFP gene 5' and 3' ends linked to the Chinook salmon growth hormone (GH) gene (EO-1α) was examined for GH mRNA. Northern blot analysis of ocean pout demonstrated that AFP mRNA was detected in most tissues with extremely high levels observed in the liver, and high levels in the stomach and gill. Low AFP mRNA levels were observed in skin, mouth skin, intestine, spleen, and kidney, while barely detectable levels were observed in heart, ovary, brain, and blood cells. No AFP mRNA was observed in muscle. For t-OP5α transgenic Atlantic salmon, AFP mRNA was detected in most tissues with high levels observed in heart, liver, stomach, and brain, low levels observed in mouth skin, intestine, spleen, gill, and muscle, and barely detectable levels observed in kidney, ovary and skin. No AFP mRNA was observed in blood cells. Northern blot analysis of EO-1α transgenic salmon demonstrated that expression was observed only in pituitary and spleen, with moderate expression detected in the pituitary and low expression in the spleen. Reverse transcription polymerase chain reaction (RT-PCR) demonstrated that the OP5α gene is expressed in all tissues of the ocean pout, and all tissues except for blood in both transgenic Atlantic salmon lines (t-OP5α and EO-1α). Blood plasma samples from the three strains of fish were tested for AFP activity by determining the thermal hysteresis (TH) for each. TH measurements from winter acclimated ocean pout were high (approximately 1.36°C) with typical bipyrimidal ice crystal shaping indicative of a high amount of AFP, while t-OP5α transgenic fish showed little(< 0.01°C) TH activity, but yielded hexagonal shaped ice crystals indicative of the presence of AFP. On the other hand, EO-1α transgenics showed no (0°C) ... |
format |
Thesis |
author |
Hobbs, Rodney Stephen |
spellingShingle |
Hobbs, Rodney Stephen The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) |
author_facet |
Hobbs, Rodney Stephen |
author_sort |
Hobbs, Rodney Stephen |
title |
The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) |
title_short |
The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) |
title_full |
The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) |
title_fullStr |
The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) |
title_full_unstemmed |
The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar) |
title_sort |
ocean pout (macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic atlantic salmon (salmo salar) |
publisher |
Memorial University of Newfoundland |
publishDate |
2005 |
url |
https://research.library.mun.ca/10662/ https://research.library.mun.ca/10662/1/Hobbs_RodneyStephen.pdf |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
https://research.library.mun.ca/10662/1/Hobbs_RodneyStephen.pdf Hobbs, Rodney Stephen <https://research.library.mun.ca/view/creator_az/Hobbs=3ARodney_Stephen=3A=3A.html> (2005) The ocean pout (Macrozoarces americanus) antifreeze protein gene promoter drives expression of antifreeze protein and growth hormone genes in transgenic Atlantic salmon (Salmo salar). Masters thesis, Memorial University of Newfoundland. |
op_rights |
thesis_license |
_version_ |
1778522584359370752 |