Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA
The European eelAnguilla anguilla is a critically endangered catadromous species. There is an urgent need for close surveillance of the populations that are still viable in European rivers. The species is difficult to observe in freshwater because of its bottom-dwelling behavior; the currently emplo...
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2023
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ftmdpi:oai:mdpi.com:/2410-3888/8/6/279/ 2023-08-20T03:59:43+02:00 Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA Sara Fernandez Álvaro Gutiérrez Dumas Deconinck Jose Luis Martinez Almudena Alvarez Isabel Marquez Gonzalo Machado-Schiaffino Eva Garcia-Vazquez agris 2023-05-24 application/pdf https://doi.org/10.3390/fishes8060279 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/fishes8060279 https://creativecommons.org/licenses/by/4.0/ Fishes; Volume 8; Issue 6; Pages: 279 Anguilla anguilla biomass prediction environmental DNA hydrographic correction qPCR specific marker Text 2023 ftmdpi https://doi.org/10.3390/fishes8060279 2023-08-01T10:12:22Z The European eelAnguilla anguilla is a critically endangered catadromous species. There is an urgent need for close surveillance of the populations that are still viable in European rivers. The species is difficult to observe in freshwater because of its bottom-dwelling behavior; the currently employed methods of eel monitoring in Europe based on the physical capture of individuals are stressful and may cause mortality. Here, we present a new highly sensitive method based on an A. anguilla-specific qPCR marker designed within the cytochrome oxidase I mitochondrial gene for application on environmental DNA (eDNA). Since the detectability of eDNA depends on the hydrographic conditions, we applied correction for altitude and a linear model and were able to predict the eel biomass from the eDNA in the different rivers of northern Spain still holding wild populations. The method was validated by electrofishing surveys. This novel eDNA-based marker allows for estimating the European eel biomass in running waters from small 1.5 L water samples and could complement, or replace in some cases, current eel surveys without disturbing wild populations. Text Anguilla anguilla European eel MDPI Open Access Publishing Fishes 8 6 279 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Anguilla anguilla biomass prediction environmental DNA hydrographic correction qPCR specific marker |
spellingShingle |
Anguilla anguilla biomass prediction environmental DNA hydrographic correction qPCR specific marker Sara Fernandez Álvaro Gutiérrez Dumas Deconinck Jose Luis Martinez Almudena Alvarez Isabel Marquez Gonzalo Machado-Schiaffino Eva Garcia-Vazquez Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA |
topic_facet |
Anguilla anguilla biomass prediction environmental DNA hydrographic correction qPCR specific marker |
description |
The European eelAnguilla anguilla is a critically endangered catadromous species. There is an urgent need for close surveillance of the populations that are still viable in European rivers. The species is difficult to observe in freshwater because of its bottom-dwelling behavior; the currently employed methods of eel monitoring in Europe based on the physical capture of individuals are stressful and may cause mortality. Here, we present a new highly sensitive method based on an A. anguilla-specific qPCR marker designed within the cytochrome oxidase I mitochondrial gene for application on environmental DNA (eDNA). Since the detectability of eDNA depends on the hydrographic conditions, we applied correction for altitude and a linear model and were able to predict the eel biomass from the eDNA in the different rivers of northern Spain still holding wild populations. The method was validated by electrofishing surveys. This novel eDNA-based marker allows for estimating the European eel biomass in running waters from small 1.5 L water samples and could complement, or replace in some cases, current eel surveys without disturbing wild populations. |
format |
Text |
author |
Sara Fernandez Álvaro Gutiérrez Dumas Deconinck Jose Luis Martinez Almudena Alvarez Isabel Marquez Gonzalo Machado-Schiaffino Eva Garcia-Vazquez |
author_facet |
Sara Fernandez Álvaro Gutiérrez Dumas Deconinck Jose Luis Martinez Almudena Alvarez Isabel Marquez Gonzalo Machado-Schiaffino Eva Garcia-Vazquez |
author_sort |
Sara Fernandez |
title |
Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA |
title_short |
Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA |
title_full |
Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA |
title_fullStr |
Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA |
title_full_unstemmed |
Biomass Quantification of the Critically Endangered European eel from Running Waters Using Environmental DNA |
title_sort |
biomass quantification of the critically endangered european eel from running waters using environmental dna |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/fishes8060279 |
op_coverage |
agris |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Fishes; Volume 8; Issue 6; Pages: 279 |
op_relation |
https://dx.doi.org/10.3390/fishes8060279 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/fishes8060279 |
container_title |
Fishes |
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8 |
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6 |
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279 |
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1774715001520521216 |