Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development

Fish reproduction is closely related to the regulation of the brain and liver, making it essential to identify the factors that control this process. The turbot (Scophthalmus maximus) is an economically significant species that has been successfully breeding through industrial aquaculture. Investiga...

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Published in:Fishes
Main Authors: Chunyan Zhao, Sujie Zheng, Yongji Dang, Mengshu Wang, Yichao Ren
Format: Text
Language:English
Published: Multidisciplinary Digital Publishing Institute 2023
Subjects:
Online Access:https://doi.org/10.3390/fishes8050240
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spelling ftmdpi:oai:mdpi.com:/2410-3888/8/5/240/ 2023-08-20T04:09:39+02:00 Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development Chunyan Zhao Sujie Zheng Yongji Dang Mengshu Wang Yichao Ren agris 2023-05-04 application/pdf https://doi.org/10.3390/fishes8050240 EN eng Multidisciplinary Digital Publishing Institute Genetics and Biotechnology https://dx.doi.org/10.3390/fishes8050240 https://creativecommons.org/licenses/by/4.0/ Fishes; Volume 8; Issue 5; Pages: 240 Scophthalmus maximus insulin-like growth factor 3 brain gonadal development Text 2023 ftmdpi https://doi.org/10.3390/fishes8050240 2023-08-01T09:56:39Z Fish reproduction is closely related to the regulation of the brain and liver, making it essential to identify the factors that control this process. The turbot (Scophthalmus maximus) is an economically significant species that has been successfully breeding through industrial aquaculture. Investigation of factors into the involvement of gonadal development is crucial for artificial breeding. In this study, a new insulin-like growth factor 3 igf3 gene was cloned and characterized. Additionally, all three types of turbot IGFs contain a distinct IGF domain, with IGF3 and IGF2 being grouped with other teleosts, demonstrating a closely related genetic relationship. The expression analysis showed that igf3 mRNA is predominantly expressed in the gonad and brain (specifically in the pituitary and hypothalamus), suggesting its effects at multiple levels in the brain–pituitary–gonadal axis. Furthermore, the mRNA levels of igfs during gonadal development were examined. In the gonad and liver of female turbots, the expression levels of igfs mRNA significantly increased from stage II to VI during the process of oogenesis, including maturation and degeneration. In the testis and liver of male turbots, igf3 mRNA maintained high expression levels during the proliferation of spermatogonia at stages II and III. In addition, the highest levels of igf1 and igf2 were observed at the beginning of spermatogenesis and during sperm production at stages V and VI. All the results suggest that the IGFs were closely related to the gonadal development in turbot and improve a better understanding of the IGF system in the regulation of gonadal development in teleost. Text Scophthalmus maximus Turbot MDPI Open Access Publishing Fishes 8 5 240
institution Open Polar
collection MDPI Open Access Publishing
op_collection_id ftmdpi
language English
topic Scophthalmus maximus
insulin-like growth factor 3
brain
gonadal development
spellingShingle Scophthalmus maximus
insulin-like growth factor 3
brain
gonadal development
Chunyan Zhao
Sujie Zheng
Yongji Dang
Mengshu Wang
Yichao Ren
Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development
topic_facet Scophthalmus maximus
insulin-like growth factor 3
brain
gonadal development
description Fish reproduction is closely related to the regulation of the brain and liver, making it essential to identify the factors that control this process. The turbot (Scophthalmus maximus) is an economically significant species that has been successfully breeding through industrial aquaculture. Investigation of factors into the involvement of gonadal development is crucial for artificial breeding. In this study, a new insulin-like growth factor 3 igf3 gene was cloned and characterized. Additionally, all three types of turbot IGFs contain a distinct IGF domain, with IGF3 and IGF2 being grouped with other teleosts, demonstrating a closely related genetic relationship. The expression analysis showed that igf3 mRNA is predominantly expressed in the gonad and brain (specifically in the pituitary and hypothalamus), suggesting its effects at multiple levels in the brain–pituitary–gonadal axis. Furthermore, the mRNA levels of igfs during gonadal development were examined. In the gonad and liver of female turbots, the expression levels of igfs mRNA significantly increased from stage II to VI during the process of oogenesis, including maturation and degeneration. In the testis and liver of male turbots, igf3 mRNA maintained high expression levels during the proliferation of spermatogonia at stages II and III. In addition, the highest levels of igf1 and igf2 were observed at the beginning of spermatogenesis and during sperm production at stages V and VI. All the results suggest that the IGFs were closely related to the gonadal development in turbot and improve a better understanding of the IGF system in the regulation of gonadal development in teleost.
format Text
author Chunyan Zhao
Sujie Zheng
Yongji Dang
Mengshu Wang
Yichao Ren
author_facet Chunyan Zhao
Sujie Zheng
Yongji Dang
Mengshu Wang
Yichao Ren
author_sort Chunyan Zhao
title Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development
title_short Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development
title_full Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development
title_fullStr Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development
title_full_unstemmed Identification of a New Insulin-like Growth Factor 3 (igf3) in Turbot (Scophthalmus maximus): Comparison and Expression Analysis of IGF System Genes during Gonadal Development
title_sort identification of a new insulin-like growth factor 3 (igf3) in turbot (scophthalmus maximus): comparison and expression analysis of igf system genes during gonadal development
publisher Multidisciplinary Digital Publishing Institute
publishDate 2023
url https://doi.org/10.3390/fishes8050240
op_coverage agris
genre Scophthalmus maximus
Turbot
genre_facet Scophthalmus maximus
Turbot
op_source Fishes; Volume 8; Issue 5; Pages: 240
op_relation Genetics and Biotechnology
https://dx.doi.org/10.3390/fishes8050240
op_rights https://creativecommons.org/licenses/by/4.0/
op_doi https://doi.org/10.3390/fishes8050240
container_title Fishes
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