Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species
In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius),...
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ftmdpi:oai:mdpi.com:/2410-3888/7/3/117/ 2023-08-20T04:09:26+02:00 Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero agris 2022-05-24 application/pdf https://doi.org/10.3390/fishes7030117 EN eng Multidisciplinary Digital Publishing Institute Genetics and Biotechnology https://dx.doi.org/10.3390/fishes7030117 https://creativecommons.org/licenses/by/4.0/ Fishes; Volume 7; Issue 3; Pages: 117 meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population Text 2022 ftmdpi https://doi.org/10.3390/fishes7030117 2023-08-01T05:08:44Z In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups. Text Red drum Sciaenops ocellatus MDPI Open Access Publishing Fishes 7 3 117 |
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collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population |
spellingShingle |
meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species |
topic_facet |
meagre ( Argyrosomus regius ) microsatellites parental assignment PCR population |
description |
In this study, a microsatellite-based multiplex PCR panel for meagre (Argyrosomus regius) was developed as a useful and single tool in parental assignment and population studies. Twenty-one specific and interspecific microsatellites from different aquaculture species of meagre (Argyrosomus regius), Japanese meagre (A. japonicus), red drum (Sciaenops ocellatus) and yellow meagre (Acoupa weakfish) were assessed for genetic variability, allelic range and genotype reliability. Finally, a SuperMultiplex for Argyrosomus regius (SMAr) was designed with only the best eight microsatellite markers. The panel assessment was performed using a batch of brood stock from one company and a sample of 616 offspring. It was possible to assign 95% of the offspring to a single pair of parents using the exclusion method. It is therefore considered an easy procedure, and a powerful and low-cost tool for parental assignment to support companies’ breeding programs and to exchange information between research groups. |
format |
Text |
author |
Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero |
author_facet |
Antonio Vallecillos Emilio María-Dolores Javier Villa Francisco Miguel Rueda José Carrillo Guillermo Ramis Mohamed Soula Juan Manuel Afonso Eva Armero |
author_sort |
Antonio Vallecillos |
title |
Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species |
title_short |
Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species |
title_full |
Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species |
title_fullStr |
Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species |
title_full_unstemmed |
Development of the First Microsatellite Multiplex PCR Panel for Meagre (Argyrosomus regius), a Commercial Aquaculture Species |
title_sort |
development of the first microsatellite multiplex pcr panel for meagre (argyrosomus regius), a commercial aquaculture species |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/fishes7030117 |
op_coverage |
agris |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_source |
Fishes; Volume 7; Issue 3; Pages: 117 |
op_relation |
Genetics and Biotechnology https://dx.doi.org/10.3390/fishes7030117 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/fishes7030117 |
container_title |
Fishes |
container_volume |
7 |
container_issue |
3 |
container_start_page |
117 |
_version_ |
1774722392544772096 |