The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products
Scarce research into the secondary metabolites of the fungi Pseudogymnoascus spp. has shown a hidden biosynthetic potential for biologically active compounds. This work investigated the biosynthesis of secondary metabolites by two Pseudogymnoascus fungal strains, VKM F-4518 and VKM F-4519, isolated...
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ftmdpi:oai:mdpi.com:/2311-5637/9/8/702/ 2023-08-20T04:04:01+02:00 The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein agris 2023-07-26 application/pdf https://doi.org/10.3390/fermentation9080702 EN eng Multidisciplinary Digital Publishing Institute Fermentation Process Design https://dx.doi.org/10.3390/fermentation9080702 https://creativecommons.org/licenses/by/4.0/ Fermentation; Volume 9; Issue 8; Pages: 702 fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B the Arctic NMR spectroscopy gene cluster Text 2023 ftmdpi https://doi.org/10.3390/fermentation9080702 2023-08-01T11:00:47Z Scarce research into the secondary metabolites of the fungi Pseudogymnoascus spp. has shown a hidden biosynthetic potential for biologically active compounds. This work investigated the biosynthesis of secondary metabolites by two Pseudogymnoascus fungal strains, VKM F-4518 and VKM F-4519, isolated from the surface soil layer of the Kolyma Lowland, Russia, in the Arctic. In these strains, 16-membered trilactone macrolides, (+)-macrosphelides A and B, were identified using 1D and 2D NMR, UHRMS, and optical rotation data. In the fungi of this genus, these metabolites were found for the first time. The studied strains are highly active producers of macrosphelide A, which is being considered as a promising agent for the cure of cancer. Using the antiSMASH secondary metabolite analysis tool, we found that the genome of strain VKM F-4518 contained 32 of the biosynthetic clusters of the secondary metabolite genes (BGC) and that of VKM F-4519 had 17 BGCs. Based on the comparison of the cluster of macrotriolide genes from the fungus Paraphaeosphaeria sporulosa, we found the complete supposed cluster BGCs of macrosphelides in the genomes of two Pseudogymnoascus strains using the BLAST+ program. Text Arctic MDPI Open Access Publishing Arctic Kolyma ENVELOPE(161.000,161.000,69.500,69.500) Fermentation 9 8 702 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B the Arctic NMR spectroscopy gene cluster |
spellingShingle |
fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B the Arctic NMR spectroscopy gene cluster Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
topic_facet |
fungi secondary metabolites natural compounds biotechnology macrolides macrosphelides A and B the Arctic NMR spectroscopy gene cluster |
description |
Scarce research into the secondary metabolites of the fungi Pseudogymnoascus spp. has shown a hidden biosynthetic potential for biologically active compounds. This work investigated the biosynthesis of secondary metabolites by two Pseudogymnoascus fungal strains, VKM F-4518 and VKM F-4519, isolated from the surface soil layer of the Kolyma Lowland, Russia, in the Arctic. In these strains, 16-membered trilactone macrolides, (+)-macrosphelides A and B, were identified using 1D and 2D NMR, UHRMS, and optical rotation data. In the fungi of this genus, these metabolites were found for the first time. The studied strains are highly active producers of macrosphelide A, which is being considered as a promising agent for the cure of cancer. Using the antiSMASH secondary metabolite analysis tool, we found that the genome of strain VKM F-4518 contained 32 of the biosynthetic clusters of the secondary metabolite genes (BGC) and that of VKM F-4519 had 17 BGCs. Based on the comparison of the cluster of macrotriolide genes from the fungus Paraphaeosphaeria sporulosa, we found the complete supposed cluster BGCs of macrosphelides in the genomes of two Pseudogymnoascus strains using the BLAST+ program. |
format |
Text |
author |
Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein |
author_facet |
Tatiana V. Antipova Kirill V. Zaitsev Valentina P. Zhelifonova Sergey V. Tarlachkov Yuri K. Grishin Galina A. Kochkina Mikhail B. Vainshtein |
author_sort |
Tatiana V. Antipova |
title |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_short |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_full |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_fullStr |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_full_unstemmed |
The Potential of Arctic Pseudogymnoascus Fungi in the Biosynthesis of Natural Products |
title_sort |
potential of arctic pseudogymnoascus fungi in the biosynthesis of natural products |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/fermentation9080702 |
op_coverage |
agris |
long_lat |
ENVELOPE(161.000,161.000,69.500,69.500) |
geographic |
Arctic Kolyma |
geographic_facet |
Arctic Kolyma |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Fermentation; Volume 9; Issue 8; Pages: 702 |
op_relation |
Fermentation Process Design https://dx.doi.org/10.3390/fermentation9080702 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/fermentation9080702 |
container_title |
Fermentation |
container_volume |
9 |
container_issue |
8 |
container_start_page |
702 |
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1774714452832157696 |