Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel
Surface modification plays a key role in the fabrication of highly active and stable enzymatic nanoreactors. In this study, we report for the first time the effect of various functional groups (epoxy, amine, trimethyl, and hexadecyl) on the catalytic performance of lipase B from Candida antarctica (...
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ftmdpi:oai:mdpi.com:/2310-2861/8/1/3/ 2023-08-20T04:01:35+02:00 Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel Cédric Decarpigny Anne Ponchel Eric Monflier Rudina Bleta 2021-12-21 application/pdf https://doi.org/10.3390/gels8010003 EN eng Multidisciplinary Digital Publishing Institute Gel Chemistry and Physics https://dx.doi.org/10.3390/gels8010003 https://creativecommons.org/licenses/by/4.0/ Gels; Volume 8; Issue 1; Pages: 3 CALB supramolecular hydrogel silica surface functionalization α-cyclodextrin Pluronic F127 2,5-furandicarboxylic acid Text 2021 ftmdpi https://doi.org/10.3390/gels8010003 2023-08-01T03:37:13Z Surface modification plays a key role in the fabrication of highly active and stable enzymatic nanoreactors. In this study, we report for the first time the effect of various functional groups (epoxy, amine, trimethyl, and hexadecyl) on the catalytic performance of lipase B from Candida antarctica (CALB) incorporated within a monolithic supramolecular hydrogel with multiscale pore architecture. The supramolecular hydrogel formed by host-guest interactions between α-cyclodextrin (α-CD) and Pluronic F127 was first silicified to provide a hierarchically porous material whose surface was further modified with different organosilanes permitting both covalent anchoring and interfacial activation of CALB. The catalytic activity of nanoreactors was evaluated in the liquid phase cascade oxidation of 2,5-diformylfuran (DFF) to 2,5-furandicarboxylic acid (FDCA) under mild conditions. Results showed that high FDCA yields and high efficiency conversion of DFF could be correlated with the ability of epoxy and amine moieties to keep CALB attached to the carrier, while the trimethyl and hexadecyl groups could provide a suitable hydrophobic-hydrophilic interface for the interfacial activation of lipase. Cationic cross-linked β-CD was also evaluated as an enzyme-stabilizing agent and was found to provide beneficial effects in the operational stability of the biocatalyst. These supramolecular silicified hydrogel monoliths with hierarchical porosity may be used as promising nanoreactors to provide easier enzyme recovery in other biocatalytic continuous flow processes. Text Antarc* Antarctica MDPI Open Access Publishing Gels 8 1 3 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
CALB supramolecular hydrogel silica surface functionalization α-cyclodextrin Pluronic F127 2,5-furandicarboxylic acid |
spellingShingle |
CALB supramolecular hydrogel silica surface functionalization α-cyclodextrin Pluronic F127 2,5-furandicarboxylic acid Cédric Decarpigny Anne Ponchel Eric Monflier Rudina Bleta Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel |
topic_facet |
CALB supramolecular hydrogel silica surface functionalization α-cyclodextrin Pluronic F127 2,5-furandicarboxylic acid |
description |
Surface modification plays a key role in the fabrication of highly active and stable enzymatic nanoreactors. In this study, we report for the first time the effect of various functional groups (epoxy, amine, trimethyl, and hexadecyl) on the catalytic performance of lipase B from Candida antarctica (CALB) incorporated within a monolithic supramolecular hydrogel with multiscale pore architecture. The supramolecular hydrogel formed by host-guest interactions between α-cyclodextrin (α-CD) and Pluronic F127 was first silicified to provide a hierarchically porous material whose surface was further modified with different organosilanes permitting both covalent anchoring and interfacial activation of CALB. The catalytic activity of nanoreactors was evaluated in the liquid phase cascade oxidation of 2,5-diformylfuran (DFF) to 2,5-furandicarboxylic acid (FDCA) under mild conditions. Results showed that high FDCA yields and high efficiency conversion of DFF could be correlated with the ability of epoxy and amine moieties to keep CALB attached to the carrier, while the trimethyl and hexadecyl groups could provide a suitable hydrophobic-hydrophilic interface for the interfacial activation of lipase. Cationic cross-linked β-CD was also evaluated as an enzyme-stabilizing agent and was found to provide beneficial effects in the operational stability of the biocatalyst. These supramolecular silicified hydrogel monoliths with hierarchical porosity may be used as promising nanoreactors to provide easier enzyme recovery in other biocatalytic continuous flow processes. |
format |
Text |
author |
Cédric Decarpigny Anne Ponchel Eric Monflier Rudina Bleta |
author_facet |
Cédric Decarpigny Anne Ponchel Eric Monflier Rudina Bleta |
author_sort |
Cédric Decarpigny |
title |
Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel |
title_short |
Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel |
title_full |
Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel |
title_fullStr |
Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel |
title_full_unstemmed |
Effect of Functional Group on the Catalytic Activity of Lipase B from Candida antarctica Immobilized in a Silica-Reinforced Pluronic F127/α-Cyclodextrin Hydrogel |
title_sort |
effect of functional group on the catalytic activity of lipase b from candida antarctica immobilized in a silica-reinforced pluronic f127/α-cyclodextrin hydrogel |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/gels8010003 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Gels; Volume 8; Issue 1; Pages: 3 |
op_relation |
Gel Chemistry and Physics https://dx.doi.org/10.3390/gels8010003 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/gels8010003 |
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