Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue
Cushing’s syndrome, or hypercortisolism (HC), a common endocrinopathy in adult dogs, is caused by chronic hypercortisolemia. Among different metabolic disorders, this syndrome is associated with enhanced subcutaneous lipolysis and visceral adiposity. However, effects of HC in adipose tissue, especia...
Published in: | Veterinary Sciences |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/vetsci9060250 |
id |
ftmdpi:oai:mdpi.com:/2306-7381/9/6/250/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2306-7381/9/6/250/ 2023-08-20T04:05:47+02:00 Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl agris 2022-05-25 application/pdf https://doi.org/10.3390/vetsci9060250 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/vetsci9060250 https://creativecommons.org/licenses/by/4.0/ Veterinary Sciences; Volume 9; Issue 6; Pages: 250 dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model Text 2022 ftmdpi https://doi.org/10.3390/vetsci9060250 2023-08-01T05:09:42Z Cushing’s syndrome, or hypercortisolism (HC), a common endocrinopathy in adult dogs, is caused by chronic hypercortisolemia. Among different metabolic disorders, this syndrome is associated with enhanced subcutaneous lipolysis and visceral adiposity. However, effects of HC in adipose tissue, especially regarding visceral adipose tissue (VAT), are still poorly understood. Herein, the transcriptomic effects of chronic HC on VAT of dogs were evaluated. For this, subcutaneously implanted ACTH-releasing pumps were used, followed by deep RNA sequencing of the canine VAT. Prolonged HC seems to affect a plethora of regulatory mechanisms in VAT of treated dogs, with 1190 differentially expressed genes (DEGs, p and FDR < 0.01) being found. The 691 downregulated DEGs were mostly associated with functional terms like cell adhesion and migration, intracellular signaling, immune response, extracellular matrix and angiogenesis. Treatment also appeared to modulate local glucocorticoid and insulin signaling and hormonal sensitivity, and several factors, e.g., TIMP4, FGF1, CCR2, CXCR4 and HSD11B1/2, were identified as possible important players in the glucocorticoid-related expansion of VAT. Modulation of their function during chronic HC might present interesting targets for further clinical studies. Similarities in the effects of chronic HC on VAT of dogs and humans are highlighted. Text Canis lupus MDPI Open Access Publishing Veterinary Sciences 9 6 250 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model |
spellingShingle |
dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
topic_facet |
dog ( Canis lupus familiaris ) Cushing’s syndrome atherosclerosis animal model |
description |
Cushing’s syndrome, or hypercortisolism (HC), a common endocrinopathy in adult dogs, is caused by chronic hypercortisolemia. Among different metabolic disorders, this syndrome is associated with enhanced subcutaneous lipolysis and visceral adiposity. However, effects of HC in adipose tissue, especially regarding visceral adipose tissue (VAT), are still poorly understood. Herein, the transcriptomic effects of chronic HC on VAT of dogs were evaluated. For this, subcutaneously implanted ACTH-releasing pumps were used, followed by deep RNA sequencing of the canine VAT. Prolonged HC seems to affect a plethora of regulatory mechanisms in VAT of treated dogs, with 1190 differentially expressed genes (DEGs, p and FDR < 0.01) being found. The 691 downregulated DEGs were mostly associated with functional terms like cell adhesion and migration, intracellular signaling, immune response, extracellular matrix and angiogenesis. Treatment also appeared to modulate local glucocorticoid and insulin signaling and hormonal sensitivity, and several factors, e.g., TIMP4, FGF1, CCR2, CXCR4 and HSD11B1/2, were identified as possible important players in the glucocorticoid-related expansion of VAT. Modulation of their function during chronic HC might present interesting targets for further clinical studies. Similarities in the effects of chronic HC on VAT of dogs and humans are highlighted. |
format |
Text |
author |
Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl |
author_facet |
Miguel Tavares Pereira Isabelle Martin Hubert Rehrauer Mariusz P. Kowalewski Felicitas S. Boretti Nadja S. Sieber-Ruckstuhl |
author_sort |
Miguel Tavares Pereira |
title |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_short |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_full |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_fullStr |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_full_unstemmed |
Effects of ACTH-Induced Long-Term Hypercortisolism on the Transcriptome of Canine Visceral Adipose Tissue |
title_sort |
effects of acth-induced long-term hypercortisolism on the transcriptome of canine visceral adipose tissue |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/vetsci9060250 |
op_coverage |
agris |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Veterinary Sciences; Volume 9; Issue 6; Pages: 250 |
op_relation |
https://dx.doi.org/10.3390/vetsci9060250 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/vetsci9060250 |
container_title |
Veterinary Sciences |
container_volume |
9 |
container_issue |
6 |
container_start_page |
250 |
_version_ |
1774716528258711552 |