Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens
Tengchong Snow chickens are one of the most precious, black-boned chickens in Yunnan province and usually produce black meat. However, we found a small number of white meat traits in the chicken population during feeding. In order to determine the pattern of melanin deposition and the molecular mech...
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ftmdpi:oai:mdpi.com:/2306-7381/10/5/341/ 2023-08-20T04:09:48+02:00 Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens Xiannian Zi Xuehai Ge Yixuan Zhu Yong Liu Dawei Sun Zijian Li Mengqian Liu Zhengrong You Bo Wang Jiajia Kang Tengfei Dou Changrong Ge Kun Wang agris 2023-05-11 application/pdf https://doi.org/10.3390/vetsci10050341 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/vetsci10050341 https://creativecommons.org/licenses/by/4.0/ Veterinary Sciences; Volume 10; Issue 5; Pages: 341 Tengchong Snow chicken skin luminance value (L value) melanin transcriptome candidate genes Text 2023 ftmdpi https://doi.org/10.3390/vetsci10050341 2023-08-01T10:01:49Z Tengchong Snow chickens are one of the most precious, black-boned chickens in Yunnan province and usually produce black meat. However, we found a small number of white meat traits in the chicken population during feeding. In order to determine the pattern of melanin deposition and the molecular mechanism of formation in the Tengchong Snow chicken, we measured the luminance value (L value) and melanin content in the skin of black meat chickens (Bc) and white meat chickens (Wc) using a color colorimeter, ELISA kit, and enzyme marker. The results showed that the L value of skin tissues in black meat chickens was significantly lower than that of white meat chickens, and the L value of skin tissues gradually increased with an increase in age. The melanin content of skin tissues in black meat chickens was higher than that of white meat chickens, and melanin content in the skin tissues gradually decreased with an increase in age, but this difference was not significant (p > 0.05); the L value of skin tissues in black meat chickens was negatively correlated with melanin content, and the correlation coefficient was mostly above −0.6. In addition, based on the phenotypic results, we chose to perform the comparative transcriptome profiling of skin tissues at 90 days of age. We screened a total of 44 differential genes, of which 32 were upregulated and 12 were downregulated. These DEGs were mainly involved in melanogenesis, tyrosine metabolism and RNA transport. We identified TYR, DCT, and EDNRB2 as possible master effector genes for skin pigmentation in Tengchong Snow black meat chickens through DEGs analysis. Finally, we measured the mRNA of TYR, DCT, MC1R, EDNRB2, GPR143, MITF, and TYRP1 genes through a quantitative real-time polymerase chain reaction (qPCR) and found that the mRNA of all the above seven genes decreased with increasing age. In conclusion, our study initially constructed an evaluation system for the black-boned traits of Tengchong Snow chickens and found key candidate genes regulating melanin ... Text Snow Chicken MDPI Open Access Publishing Veterinary Sciences 10 5 341 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
Tengchong Snow chicken skin luminance value (L value) melanin transcriptome candidate genes |
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Tengchong Snow chicken skin luminance value (L value) melanin transcriptome candidate genes Xiannian Zi Xuehai Ge Yixuan Zhu Yong Liu Dawei Sun Zijian Li Mengqian Liu Zhengrong You Bo Wang Jiajia Kang Tengfei Dou Changrong Ge Kun Wang Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens |
topic_facet |
Tengchong Snow chicken skin luminance value (L value) melanin transcriptome candidate genes |
description |
Tengchong Snow chickens are one of the most precious, black-boned chickens in Yunnan province and usually produce black meat. However, we found a small number of white meat traits in the chicken population during feeding. In order to determine the pattern of melanin deposition and the molecular mechanism of formation in the Tengchong Snow chicken, we measured the luminance value (L value) and melanin content in the skin of black meat chickens (Bc) and white meat chickens (Wc) using a color colorimeter, ELISA kit, and enzyme marker. The results showed that the L value of skin tissues in black meat chickens was significantly lower than that of white meat chickens, and the L value of skin tissues gradually increased with an increase in age. The melanin content of skin tissues in black meat chickens was higher than that of white meat chickens, and melanin content in the skin tissues gradually decreased with an increase in age, but this difference was not significant (p > 0.05); the L value of skin tissues in black meat chickens was negatively correlated with melanin content, and the correlation coefficient was mostly above −0.6. In addition, based on the phenotypic results, we chose to perform the comparative transcriptome profiling of skin tissues at 90 days of age. We screened a total of 44 differential genes, of which 32 were upregulated and 12 were downregulated. These DEGs were mainly involved in melanogenesis, tyrosine metabolism and RNA transport. We identified TYR, DCT, and EDNRB2 as possible master effector genes for skin pigmentation in Tengchong Snow black meat chickens through DEGs analysis. Finally, we measured the mRNA of TYR, DCT, MC1R, EDNRB2, GPR143, MITF, and TYRP1 genes through a quantitative real-time polymerase chain reaction (qPCR) and found that the mRNA of all the above seven genes decreased with increasing age. In conclusion, our study initially constructed an evaluation system for the black-boned traits of Tengchong Snow chickens and found key candidate genes regulating melanin ... |
format |
Text |
author |
Xiannian Zi Xuehai Ge Yixuan Zhu Yong Liu Dawei Sun Zijian Li Mengqian Liu Zhengrong You Bo Wang Jiajia Kang Tengfei Dou Changrong Ge Kun Wang |
author_facet |
Xiannian Zi Xuehai Ge Yixuan Zhu Yong Liu Dawei Sun Zijian Li Mengqian Liu Zhengrong You Bo Wang Jiajia Kang Tengfei Dou Changrong Ge Kun Wang |
author_sort |
Xiannian Zi |
title |
Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens |
title_short |
Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens |
title_full |
Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens |
title_fullStr |
Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens |
title_full_unstemmed |
Transcriptome Profile Analysis Identifies Candidate Genes for the Melanin Pigmentation of Skin in Tengchong Snow Chickens |
title_sort |
transcriptome profile analysis identifies candidate genes for the melanin pigmentation of skin in tengchong snow chickens |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/vetsci10050341 |
op_coverage |
agris |
genre |
Snow Chicken |
genre_facet |
Snow Chicken |
op_source |
Veterinary Sciences; Volume 10; Issue 5; Pages: 341 |
op_relation |
https://dx.doi.org/10.3390/vetsci10050341 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/vetsci10050341 |
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