Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level
The antibiotic resistance phenomenon horizontally involves numerous bacteria cultured from fresh or processed seafood matrix microbiomes. In this study, the identified bacteria from food-producing processes and industrial environments were screened for phenotypic and genotypic resistance determinant...
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ftmdpi:oai:mdpi.com:/2304-8158/12/8/1699/ 2023-08-20T04:06:38+02:00 Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level Gianluigi Ferri Carlotta Lauteri Mauro Scattolini Alberto Vergara agris 2023-04-19 application/pdf https://doi.org/10.3390/foods12081699 EN eng Multidisciplinary Digital Publishing Institute Food Microbiology https://dx.doi.org/10.3390/foods12081699 https://creativecommons.org/licenses/by/4.0/ Foods; Volume 12; Issue 8; Pages: 1699 seafood industry Gadidae salted and seasoned products soaked products high pressure processing antibiotic resistance antibiotic resistance genes one-health Text 2023 ftmdpi https://doi.org/10.3390/foods12081699 2023-08-01T09:45:25Z The antibiotic resistance phenomenon horizontally involves numerous bacteria cultured from fresh or processed seafood matrix microbiomes. In this study, the identified bacteria from food-producing processes and industrial environments were screened for phenotypic and genotypic resistance determinants. A total of 684 bacterial strains [537 from processed codfish (Gadus morhua and Gadus macrocephalus) products as salted and seasoned and soaked and 147 from environmental samples] were isolated. Antibiotic susceptibility tests showed resistance against tetracycline, oxacillin, and clindamycin in the Staphylococcus genus (both from food and environmental samples) and against beta-lactams (cefotaxime, carbapenems, etc.) and nitrofurans (nitrofurantoin) from E. coli and Salmonella enterica serovar. Enteritidis isolates. One-thousand and ten genetic determinants—tetracycline tetC (25.17%), tetK (21.06%), tetL (11.70%), clindamycin ermC (17.23%), ermB (7.60%), linezolid cfr (8.22%), optrA (3.62%), poxtA (2.05%), and oxacillin mecA (17.37%)—were amplified from Gram-positive resistant and phenotypically susceptible bacteria. Concerning Gram-negative bacteria, the beta-lactam-resistant genes (blaTEM, blaCIT, blaCTX-M, blaIMP, blaKPC, blaOXA-48-like) represented 57.30% of the amplified ARGs. This study found high antibiotic resistance genes in circulation in the fish food industry chain from the macro- to microenvironment. The obtained data confirmed the diffusion of the “antibiotic resistance phenomenon” and its repercussions on the One-health and food-producing systems. Text Gadus morhua MDPI Open Access Publishing Foods 12 8 1699 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
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seafood industry Gadidae salted and seasoned products soaked products high pressure processing antibiotic resistance antibiotic resistance genes one-health |
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seafood industry Gadidae salted and seasoned products soaked products high pressure processing antibiotic resistance antibiotic resistance genes one-health Gianluigi Ferri Carlotta Lauteri Mauro Scattolini Alberto Vergara Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level |
topic_facet |
seafood industry Gadidae salted and seasoned products soaked products high pressure processing antibiotic resistance antibiotic resistance genes one-health |
description |
The antibiotic resistance phenomenon horizontally involves numerous bacteria cultured from fresh or processed seafood matrix microbiomes. In this study, the identified bacteria from food-producing processes and industrial environments were screened for phenotypic and genotypic resistance determinants. A total of 684 bacterial strains [537 from processed codfish (Gadus morhua and Gadus macrocephalus) products as salted and seasoned and soaked and 147 from environmental samples] were isolated. Antibiotic susceptibility tests showed resistance against tetracycline, oxacillin, and clindamycin in the Staphylococcus genus (both from food and environmental samples) and against beta-lactams (cefotaxime, carbapenems, etc.) and nitrofurans (nitrofurantoin) from E. coli and Salmonella enterica serovar. Enteritidis isolates. One-thousand and ten genetic determinants—tetracycline tetC (25.17%), tetK (21.06%), tetL (11.70%), clindamycin ermC (17.23%), ermB (7.60%), linezolid cfr (8.22%), optrA (3.62%), poxtA (2.05%), and oxacillin mecA (17.37%)—were amplified from Gram-positive resistant and phenotypically susceptible bacteria. Concerning Gram-negative bacteria, the beta-lactam-resistant genes (blaTEM, blaCIT, blaCTX-M, blaIMP, blaKPC, blaOXA-48-like) represented 57.30% of the amplified ARGs. This study found high antibiotic resistance genes in circulation in the fish food industry chain from the macro- to microenvironment. The obtained data confirmed the diffusion of the “antibiotic resistance phenomenon” and its repercussions on the One-health and food-producing systems. |
format |
Text |
author |
Gianluigi Ferri Carlotta Lauteri Mauro Scattolini Alberto Vergara |
author_facet |
Gianluigi Ferri Carlotta Lauteri Mauro Scattolini Alberto Vergara |
author_sort |
Gianluigi Ferri |
title |
Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level |
title_short |
Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level |
title_full |
Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level |
title_fullStr |
Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level |
title_full_unstemmed |
Antibiotic Resistance Profiles and ARG Detection from Isolated Bacteria in a Culture-Dependent Study at the Codfish Industry Level |
title_sort |
antibiotic resistance profiles and arg detection from isolated bacteria in a culture-dependent study at the codfish industry level |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/foods12081699 |
op_coverage |
agris |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Foods; Volume 12; Issue 8; Pages: 1699 |
op_relation |
Food Microbiology https://dx.doi.org/10.3390/foods12081699 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/foods12081699 |
container_title |
Foods |
container_volume |
12 |
container_issue |
8 |
container_start_page |
1699 |
_version_ |
1774717856770949120 |