Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro
Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflamm...
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ftmdpi:oai:mdpi.com:/2304-8158/10/11/2887/ 2023-08-20T04:00:19+02:00 Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin agris 2021-11-22 application/pdf https://doi.org/10.3390/foods10112887 EN eng Multidisciplinary Digital Publishing Institute Nutraceuticals and Functional Foods https://dx.doi.org/10.3390/foods10112887 https://creativecommons.org/licenses/by/4.0/ Foods; Volume 10; Issue 11; Pages: 2887 krill oil anti-inflammatory effect RAW 264.7 cell chemical composition Text 2021 ftmdpi https://doi.org/10.3390/foods10112887 2023-08-01T03:19:39Z Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflammatory activity, there is little information about the relationship between its chemical compositions and anti-inflammatory activity. In this study, the RAW264.7 macrophages model was used to elucidate and compare the anti-inflammatory potential of different krill lipid fractions: KLF-A, KLF-H and KLF-E, which have increasing phospholipids, EPA and DHA contents but decreasing astaxanthin and tocopherols levels. Results showed that all the krill lipid fractions alleviated the inflammatory reaction by inhibition of production of nitric oxide (NO), release of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 and gene expression of proinflammatory mediators including TNF-α, IL-1β, IL-6, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). In addition, KLF-E with the highest phospholipids, EPA and DHA contents showed the strongest inhibition effect on the LPS-induced proinflammatory mediator release and their gene expressions. The results would be helpful to provide powerful insights into the underlying anti-inflammatory mechanism of krill lipid and guiding the production of krill oil products with tailor-made anti-inflammatory activity. Text Antarc* Antarctic Antarctic Krill MDPI Open Access Publishing Antarctic Foods 10 11 2887 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
krill oil anti-inflammatory effect RAW 264.7 cell chemical composition |
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krill oil anti-inflammatory effect RAW 264.7 cell chemical composition Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro |
topic_facet |
krill oil anti-inflammatory effect RAW 264.7 cell chemical composition |
description |
Antarctic krill oil is an emerging marine lipid and expected to be a potential functional food due to its diverse nutrients, such as eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), phospholipids, astaxanthin and tocopherols. Although krill oil has been previously proved to have anti-inflammatory activity, there is little information about the relationship between its chemical compositions and anti-inflammatory activity. In this study, the RAW264.7 macrophages model was used to elucidate and compare the anti-inflammatory potential of different krill lipid fractions: KLF-A, KLF-H and KLF-E, which have increasing phospholipids, EPA and DHA contents but decreasing astaxanthin and tocopherols levels. Results showed that all the krill lipid fractions alleviated the inflammatory reaction by inhibition of production of nitric oxide (NO), release of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-6 and gene expression of proinflammatory mediators including TNF-α, IL-1β, IL-6, cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). In addition, KLF-E with the highest phospholipids, EPA and DHA contents showed the strongest inhibition effect on the LPS-induced proinflammatory mediator release and their gene expressions. The results would be helpful to provide powerful insights into the underlying anti-inflammatory mechanism of krill lipid and guiding the production of krill oil products with tailor-made anti-inflammatory activity. |
format |
Text |
author |
Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin |
author_facet |
Dan Xie Fangyuan He Xiaosan Wang Xingguo Wang Qingzhe Jin Jun Jin |
author_sort |
Dan Xie |
title |
Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro |
title_short |
Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro |
title_full |
Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro |
title_fullStr |
Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro |
title_full_unstemmed |
Diverse Krill Lipid Fractions Differentially Reduce LPS-Induced Inflammatory Markers in RAW264.7 Macrophages In Vitro |
title_sort |
diverse krill lipid fractions differentially reduce lps-induced inflammatory markers in raw264.7 macrophages in vitro |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/foods10112887 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Foods; Volume 10; Issue 11; Pages: 2887 |
op_relation |
Nutraceuticals and Functional Foods https://dx.doi.org/10.3390/foods10112887 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/foods10112887 |
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Foods |
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10 |
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11 |
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2887 |
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