Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions
Application of lipases (preferentially Candida antarctica Lipase B, CALB) for melt polycondensation of aliphatic polyesters by transesterification of activated dicarboxylic acids with diols allows to displace toxic metal and metal oxide catalysts. Immobilization of the enzyme enhances the activity a...
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ftmdpi:oai:mdpi.com:/2227-9717/9/3/411/ 2023-08-20T04:01:10+02:00 Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions Doris Pospiech Renata Choińska Daniel Flugrat Karin Sahre Dieter Jehnichen Andreas Korwitz Peter Friedel Anett Werner Brigitte Voit agris 2021-02-25 application/pdf https://doi.org/10.3390/pr9030411 EN eng Multidisciplinary Digital Publishing Institute Materials Processes https://dx.doi.org/10.3390/pr9030411 https://creativecommons.org/licenses/by/4.0/ Processes; Volume 9; Issue 3; Pages: 411 Candida antarctica enzyme activity enzymatic polycondensation poly(butylene succinate) aliphatic polyester Text 2021 ftmdpi https://doi.org/10.3390/pr9030411 2023-08-01T01:08:43Z Application of lipases (preferentially Candida antarctica Lipase B, CALB) for melt polycondensation of aliphatic polyesters by transesterification of activated dicarboxylic acids with diols allows to displace toxic metal and metal oxide catalysts. Immobilization of the enzyme enhances the activity and the temperature range of use. The possibility to use enzyme-catalyzed polycondensation in melt is studied and compared to results of polycondensations in solution. The experiments show that CALB successfully catalyzes polycondensation of both, divinyladipate and dimethylsuccinate, respectively, with 1,4-butanediol. NMR spectroscopy, relative molar masses obtained by size exclusion chromatography, MALDI-TOF MS and wide-angle X-ray scattering are employed to compare the influence of synthesis conditions for poly(butylene adipate) (PBA) and poly(butylene succinate) (PBS). It is shown that the enzymatic activity of immobilized CALB deviates and influences the molar mass. CALB-catalyzed polycondensation of PBA in solution for 24 h at 70 °C achieves molar masses of up to Mw~60,000 g/mol, higher than reported previously and comparable to conventional PBA, while melt polycondensation resulted in a moderate decrease of molar mass to Mw~31,000. Enzymatically catalyzed melt polycondensation of PBS yields Mw~23,400 g/mol vs. Mw~40,000 g/mol with titanium(IV)n-butoxide. Melt polycondensation with enzyme catalysis allows to reduce the reaction time from days to 3–4 h. Text Antarc* Antarctica MDPI Open Access Publishing Processes 9 3 411 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
Candida antarctica enzyme activity enzymatic polycondensation poly(butylene succinate) aliphatic polyester |
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Candida antarctica enzyme activity enzymatic polycondensation poly(butylene succinate) aliphatic polyester Doris Pospiech Renata Choińska Daniel Flugrat Karin Sahre Dieter Jehnichen Andreas Korwitz Peter Friedel Anett Werner Brigitte Voit Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions |
topic_facet |
Candida antarctica enzyme activity enzymatic polycondensation poly(butylene succinate) aliphatic polyester |
description |
Application of lipases (preferentially Candida antarctica Lipase B, CALB) for melt polycondensation of aliphatic polyesters by transesterification of activated dicarboxylic acids with diols allows to displace toxic metal and metal oxide catalysts. Immobilization of the enzyme enhances the activity and the temperature range of use. The possibility to use enzyme-catalyzed polycondensation in melt is studied and compared to results of polycondensations in solution. The experiments show that CALB successfully catalyzes polycondensation of both, divinyladipate and dimethylsuccinate, respectively, with 1,4-butanediol. NMR spectroscopy, relative molar masses obtained by size exclusion chromatography, MALDI-TOF MS and wide-angle X-ray scattering are employed to compare the influence of synthesis conditions for poly(butylene adipate) (PBA) and poly(butylene succinate) (PBS). It is shown that the enzymatic activity of immobilized CALB deviates and influences the molar mass. CALB-catalyzed polycondensation of PBA in solution for 24 h at 70 °C achieves molar masses of up to Mw~60,000 g/mol, higher than reported previously and comparable to conventional PBA, while melt polycondensation resulted in a moderate decrease of molar mass to Mw~31,000. Enzymatically catalyzed melt polycondensation of PBS yields Mw~23,400 g/mol vs. Mw~40,000 g/mol with titanium(IV)n-butoxide. Melt polycondensation with enzyme catalysis allows to reduce the reaction time from days to 3–4 h. |
format |
Text |
author |
Doris Pospiech Renata Choińska Daniel Flugrat Karin Sahre Dieter Jehnichen Andreas Korwitz Peter Friedel Anett Werner Brigitte Voit |
author_facet |
Doris Pospiech Renata Choińska Daniel Flugrat Karin Sahre Dieter Jehnichen Andreas Korwitz Peter Friedel Anett Werner Brigitte Voit |
author_sort |
Doris Pospiech |
title |
Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions |
title_short |
Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions |
title_full |
Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions |
title_fullStr |
Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions |
title_full_unstemmed |
Enzymatic Synthesis of Poly(alkylene succinate)s: Influence of Reaction Conditions |
title_sort |
enzymatic synthesis of poly(alkylene succinate)s: influence of reaction conditions |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/pr9030411 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Processes; Volume 9; Issue 3; Pages: 411 |
op_relation |
Materials Processes https://dx.doi.org/10.3390/pr9030411 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pr9030411 |
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Processes |
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9 |
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3 |
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411 |
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1774723151182168064 |