Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation
Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental c...
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ftmdpi:oai:mdpi.com:/2227-9717/9/1/14/ 2023-08-20T04:01:01+02:00 Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation Wenyao Shao Ying Lin Yinghua Lu agris 2020-12-23 application/pdf https://doi.org/10.3390/pr9010014 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/pr9010014 https://creativecommons.org/licenses/by/4.0/ Processes; Volume 9; Issue 1; Pages: 14 foam separation fermentation lipase surfactant enrichment ratio recovery rate Text 2020 ftmdpi https://doi.org/10.3390/pr9010014 2023-08-01T00:43:16Z Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B. Text Antarc* Antarctica MDPI Open Access Publishing Processes 9 1 14 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
foam separation fermentation lipase surfactant enrichment ratio recovery rate |
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foam separation fermentation lipase surfactant enrichment ratio recovery rate Wenyao Shao Ying Lin Yinghua Lu Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
topic_facet |
foam separation fermentation lipase surfactant enrichment ratio recovery rate |
description |
Candida antarctica Lipase B (CALB) has a wide range of applications in many fields. In this study, Pichia pastoris was used to express CALB for fermentation tank culture. Sodium dodecyl sulfate (SDS) was used as a surfactant, and foam separation technology was used to explore the best experimental conditions for the harvest of CALB. The results showed that the optimal technological conditions for the foam separation and recovery of CALB were as follows: liquid volume was 150 mL, separating gas velocity was 600 mL/min, pH value was 7, and surfactant SDS concentration was 0.5 mg/mL. Under these conditions, the enrichment ratio of CALB was 0.95, and recovery rate R was 80.32%, respectively, indicating that the foam separation technology is feasible to extract lipase B. |
format |
Text |
author |
Wenyao Shao Ying Lin Yinghua Lu |
author_facet |
Wenyao Shao Ying Lin Yinghua Lu |
author_sort |
Wenyao Shao |
title |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_short |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_full |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_fullStr |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_full_unstemmed |
Study on the Extraction Technology of Candida antarctica Lipase B by Foam Separation |
title_sort |
study on the extraction technology of candida antarctica lipase b by foam separation |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/pr9010014 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Processes; Volume 9; Issue 1; Pages: 14 |
op_relation |
https://dx.doi.org/10.3390/pr9010014 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/pr9010014 |
container_title |
Processes |
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9 |
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1 |
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14 |
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1774722123781111808 |