Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone
Lipids are the fundamental components of cell membranes and they play a significant role in their integrity and fluidity. The alteration in lipid composition of membranes has been reported to be a major response to abiotic environmental stresses. Seasonal dynamics of membrane lipids in the shoots of...
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ftmdpi:oai:mdpi.com:/2223-7747/12/1/15/ 2023-08-20T04:09:15+02:00 Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone Vasiliy V. Nokhsorov Svetlana V. Senik Valentina E. Sofronova Ekaterina R. Kotlova Alexander D. Misharev Nadezhda K. Chirikova Lyubov V. Dudareva agris 2022-12-20 application/pdf https://doi.org/10.3390/plants12010015 EN eng Multidisciplinary Digital Publishing Institute Plant Physiology and Metabolism https://dx.doi.org/10.3390/plants12010015 https://creativecommons.org/licenses/by/4.0/ Plants; Volume 12; Issue 1; Pages: 15 Ephedra monosperma lipids fatty acids glycerolipid profiling molecular species seasonal dynamics cold tolerance phospholipids glycolipids adaptation lipid metabolism Text 2022 ftmdpi https://doi.org/10.3390/plants12010015 2023-08-01T07:53:02Z Lipids are the fundamental components of cell membranes and they play a significant role in their integrity and fluidity. The alteration in lipid composition of membranes has been reported to be a major response to abiotic environmental stresses. Seasonal dynamics of membrane lipids in the shoots of Ephedra monosperma J.G. Gmel. ex C.A. Mey. growing in natural conditions of permafrost ecosystems was studied using HPTLC, GC-MS and ESI-MS. An important role of lipid metabolism was established during the autumn-winter period when the shoots of the evergreen shrub were exposed to low positive (3.6 °C), negative (−8.3 °C) and extremely low temperatures (−38.4 °C). Maximum accumulation of phosphatidic acid (PA), the amount of which is times times greater than the sum of phosphatidylcholine and phosphatidylethanolamine (PC + PE) was noted in shoots of E. monosperma in the summer-autumn period. The autumn hardening period (3.6 °C) is accompanied by active biosynthesis and accumulation of membrane lipids, a decrease of saturated 34:1 PCs, 34:1 PEs and 34:1 PAs, and an increase in unsaturated long-chain 38:5 PEs, 38:6 PEs, indicating that the adaptation of E. monosperma occurs not at the level of lipid classes but at the level of molecular species. At a further decrease of average daily air temperature in October (−8.3 °C) a sharp decline of PA level was registered. At an extreme reduction of environmental temperature (−38.4 °C) the content of non-bilayer PE and PA increases, the level of unsaturated fatty acids (FA) rises due to the increase of C18:2(Δ9,12) and C18:3(Δ9,12,15) acids and the decrease of C16:0 acids. It is concluded that changes in lipid metabolism reflect structural and functional reorganization of cell membranes and are an integral component of the complex process of plant hardening to low temperatures, which contributes to the survival of E. monosperma monocotyledonous plants in the extreme conditions of the Yakutia cryolithozone. Text permafrost Yakutia MDPI Open Access Publishing Plants 12 1 15 |
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MDPI Open Access Publishing |
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English |
topic |
Ephedra monosperma lipids fatty acids glycerolipid profiling molecular species seasonal dynamics cold tolerance phospholipids glycolipids adaptation lipid metabolism |
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Ephedra monosperma lipids fatty acids glycerolipid profiling molecular species seasonal dynamics cold tolerance phospholipids glycolipids adaptation lipid metabolism Vasiliy V. Nokhsorov Svetlana V. Senik Valentina E. Sofronova Ekaterina R. Kotlova Alexander D. Misharev Nadezhda K. Chirikova Lyubov V. Dudareva Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone |
topic_facet |
Ephedra monosperma lipids fatty acids glycerolipid profiling molecular species seasonal dynamics cold tolerance phospholipids glycolipids adaptation lipid metabolism |
description |
Lipids are the fundamental components of cell membranes and they play a significant role in their integrity and fluidity. The alteration in lipid composition of membranes has been reported to be a major response to abiotic environmental stresses. Seasonal dynamics of membrane lipids in the shoots of Ephedra monosperma J.G. Gmel. ex C.A. Mey. growing in natural conditions of permafrost ecosystems was studied using HPTLC, GC-MS and ESI-MS. An important role of lipid metabolism was established during the autumn-winter period when the shoots of the evergreen shrub were exposed to low positive (3.6 °C), negative (−8.3 °C) and extremely low temperatures (−38.4 °C). Maximum accumulation of phosphatidic acid (PA), the amount of which is times times greater than the sum of phosphatidylcholine and phosphatidylethanolamine (PC + PE) was noted in shoots of E. monosperma in the summer-autumn period. The autumn hardening period (3.6 °C) is accompanied by active biosynthesis and accumulation of membrane lipids, a decrease of saturated 34:1 PCs, 34:1 PEs and 34:1 PAs, and an increase in unsaturated long-chain 38:5 PEs, 38:6 PEs, indicating that the adaptation of E. monosperma occurs not at the level of lipid classes but at the level of molecular species. At a further decrease of average daily air temperature in October (−8.3 °C) a sharp decline of PA level was registered. At an extreme reduction of environmental temperature (−38.4 °C) the content of non-bilayer PE and PA increases, the level of unsaturated fatty acids (FA) rises due to the increase of C18:2(Δ9,12) and C18:3(Δ9,12,15) acids and the decrease of C16:0 acids. It is concluded that changes in lipid metabolism reflect structural and functional reorganization of cell membranes and are an integral component of the complex process of plant hardening to low temperatures, which contributes to the survival of E. monosperma monocotyledonous plants in the extreme conditions of the Yakutia cryolithozone. |
format |
Text |
author |
Vasiliy V. Nokhsorov Svetlana V. Senik Valentina E. Sofronova Ekaterina R. Kotlova Alexander D. Misharev Nadezhda K. Chirikova Lyubov V. Dudareva |
author_facet |
Vasiliy V. Nokhsorov Svetlana V. Senik Valentina E. Sofronova Ekaterina R. Kotlova Alexander D. Misharev Nadezhda K. Chirikova Lyubov V. Dudareva |
author_sort |
Vasiliy V. Nokhsorov |
title |
Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone |
title_short |
Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone |
title_full |
Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone |
title_fullStr |
Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone |
title_full_unstemmed |
Role of Lipids of the Evergreen Shrub Ephedra monosperma in Adaptation to Low Temperature in the Cryolithozone |
title_sort |
role of lipids of the evergreen shrub ephedra monosperma in adaptation to low temperature in the cryolithozone |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/plants12010015 |
op_coverage |
agris |
genre |
permafrost Yakutia |
genre_facet |
permafrost Yakutia |
op_source |
Plants; Volume 12; Issue 1; Pages: 15 |
op_relation |
Plant Physiology and Metabolism https://dx.doi.org/10.3390/plants12010015 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/plants12010015 |
container_title |
Plants |
container_volume |
12 |
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1 |
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15 |
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