Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein
Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence...
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ftmdpi:oai:mdpi.com:/2218-273X/9/4/139/ 2023-08-20T04:03:49+02:00 Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein Aya Yamazaki Yoshiyuki Nishimiya Sakae Tsuda Koji Togashi Hiroyuki Munehara agris 2019-04-06 application/pdf https://doi.org/10.3390/biom9040139 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/biom9040139 https://creativecommons.org/licenses/by/4.0/ Biomolecules; Volume 9; Issue 4; Pages: 139 antifreeze proteins cold adaptations Cottoidea thermal hysteresis Text 2019 ftmdpi https://doi.org/10.3390/biom9040139 2023-07-31T22:10:30Z Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence encoding type I AFP for 3 species of 2 families (Cottidae and Agonidae) belonging to Cottoidea. We also examined antifreeze activity for 3 families and 32 species of Cottoidea (Cottidae, Agonidae, and Rhamphocottidae). These fishes were collected in 2013–2015 from the Arctic Ocean, Alaska, Japan. We could identify 8 distinct DNA sequences exhibiting a high similarity to those reported for Myoxocephalus species, suggesting that Cottidae and Agonidae share the same DNA sequence encoding type I AFP. Among the 3 families, Rhamphocottidae that experience a warm current did not show antifreeze activity. The species inhabiting the Arctic Ocean and Northern Japan that often covered with ice floe showed high activity, while those inhabiting Alaska, Southern Japan with a warm current showed low/no activity. These results suggest that Cottoidea acquires type I AFP gene before dividing into Cottidae and Agonidae, and have adapted to each location with optimal antifreeze activity level. Text Arctic Arctic Ocean Alaska MDPI Open Access Publishing Arctic Arctic Ocean Pacific Biomolecules 9 4 139 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
antifreeze proteins cold adaptations Cottoidea thermal hysteresis |
spellingShingle |
antifreeze proteins cold adaptations Cottoidea thermal hysteresis Aya Yamazaki Yoshiyuki Nishimiya Sakae Tsuda Koji Togashi Hiroyuki Munehara Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
topic_facet |
antifreeze proteins cold adaptations Cottoidea thermal hysteresis |
description |
Many marine species inhabiting icy seawater produce antifreeze proteins (AFPs) to prevent their body fluids from freezing. The sculpin species of the superfamily Cottoidea are widely found from the Arctic to southern hemisphere, some of which are known to express AFP. Here we clarified DNA sequence encoding type I AFP for 3 species of 2 families (Cottidae and Agonidae) belonging to Cottoidea. We also examined antifreeze activity for 3 families and 32 species of Cottoidea (Cottidae, Agonidae, and Rhamphocottidae). These fishes were collected in 2013–2015 from the Arctic Ocean, Alaska, Japan. We could identify 8 distinct DNA sequences exhibiting a high similarity to those reported for Myoxocephalus species, suggesting that Cottidae and Agonidae share the same DNA sequence encoding type I AFP. Among the 3 families, Rhamphocottidae that experience a warm current did not show antifreeze activity. The species inhabiting the Arctic Ocean and Northern Japan that often covered with ice floe showed high activity, while those inhabiting Alaska, Southern Japan with a warm current showed low/no activity. These results suggest that Cottoidea acquires type I AFP gene before dividing into Cottidae and Agonidae, and have adapted to each location with optimal antifreeze activity level. |
format |
Text |
author |
Aya Yamazaki Yoshiyuki Nishimiya Sakae Tsuda Koji Togashi Hiroyuki Munehara |
author_facet |
Aya Yamazaki Yoshiyuki Nishimiya Sakae Tsuda Koji Togashi Hiroyuki Munehara |
author_sort |
Aya Yamazaki |
title |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_short |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_full |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_fullStr |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_full_unstemmed |
Freeze Tolerance in Sculpins (Pisces; Cottoidea) Inhabiting North Pacific and Arctic Oceans: Antifreeze Activity and Gene Sequences of the Antifreeze Protein |
title_sort |
freeze tolerance in sculpins (pisces; cottoidea) inhabiting north pacific and arctic oceans: antifreeze activity and gene sequences of the antifreeze protein |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/biom9040139 |
op_coverage |
agris |
geographic |
Arctic Arctic Ocean Pacific |
geographic_facet |
Arctic Arctic Ocean Pacific |
genre |
Arctic Arctic Ocean Alaska |
genre_facet |
Arctic Arctic Ocean Alaska |
op_source |
Biomolecules; Volume 9; Issue 4; Pages: 139 |
op_relation |
https://dx.doi.org/10.3390/biom9040139 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biom9040139 |
container_title |
Biomolecules |
container_volume |
9 |
container_issue |
4 |
container_start_page |
139 |
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1774714255487008768 |