Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research
Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and...
Published in: | Metabolites |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/metabo12010028 |
id |
ftmdpi:oai:mdpi.com:/2218-1989/12/1/28/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2218-1989/12/1/28/ 2023-08-20T04:05:17+02:00 Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig 2021-12-31 application/pdf https://doi.org/10.3390/metabo12010028 EN eng Multidisciplinary Digital Publishing Institute Animal Metabolism https://dx.doi.org/10.3390/metabo12010028 https://creativecommons.org/licenses/by/4.0/ Metabolites; Volume 12; Issue 1; Pages: 28 gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Text 2021 ftmdpi https://doi.org/10.3390/metabo12010028 2023-08-01T03:43:12Z Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon (Salmo salar) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research. Text Atlantic salmon Salmo salar MDPI Open Access Publishing Metabolites 12 1 28 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization |
spellingShingle |
gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
topic_facet |
gill mucus skin mucus Atlantic salmon biomarkers non-invasive sampling data normalization |
description |
Mucous membranes such as the gill and skin mucosa in fish protect them against a multitude of environmental factors. At the same time, changes in the molecular composition of mucus may provide valuable information about the interaction of the fish with their environment, as well as their health and welfare. In this study, the metabolite profiles of the plasma, skin and gill mucus of freshwater Atlantic salmon (Salmo salar) were compared using liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS). Several normalization procedures aimed to reduce unwanted variation in the untargeted data were tested. In addition, the basal metabolism of skin and gills, and the impact of the anesthetic benzocaine for euthanisation were studied. For targeted metabolomics, the commercial AbsoluteIDQ p400 HR kit was used to evaluate the potential differences in metabolic composition in epidermal mucus as compared to the plasma. The targeted metabolomics data showed a high level of correlation between different types of biological fluids from the same individual, indicating that mucus metabolite composition could be used for fish health monitoring and research. |
format |
Text |
author |
Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig |
author_facet |
Lada Ivanova Oscar D. Rangel-Huerta Haitham Tartor Mona C. Gjessing Maria K. Dahle Silvio Uhlig |
author_sort |
Lada Ivanova |
title |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_short |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_full |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_fullStr |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_full_unstemmed |
Fish Skin and Gill Mucus: A Source of Metabolites for Non-Invasive Health Monitoring and Research |
title_sort |
fish skin and gill mucus: a source of metabolites for non-invasive health monitoring and research |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/metabo12010028 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Metabolites; Volume 12; Issue 1; Pages: 28 |
op_relation |
Animal Metabolism https://dx.doi.org/10.3390/metabo12010028 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/metabo12010028 |
container_title |
Metabolites |
container_volume |
12 |
container_issue |
1 |
container_start_page |
28 |
_version_ |
1774715781412552704 |