Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon
The overexpression of GATA-3, T-bet and TGF-ß may theoretically induce IL-4/A, IFN-γ and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ß were used as overexpression tools, transfected...
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ftmdpi:oai:mdpi.com:/2079-7737/9/4/82/ 2023-08-20T04:05:18+02:00 Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon Tiril H. Slettjord Hege J. Sekkenes Heng Chi Jarl Bøgwald Trilochan Swain Roy A. Dalmo Jaya Kumari Swain agris 2020-04-20 application/pdf https://doi.org/10.3390/biology9040082 EN eng Multidisciplinary Digital Publishing Institute Immunology https://dx.doi.org/10.3390/biology9040082 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 9; Issue 4; Pages: 82 overexpression T-bet GATA-3 TGF-ß Atlantic salmon gene expression Text 2020 ftmdpi https://doi.org/10.3390/biology9040082 2023-07-31T23:23:53Z The overexpression of GATA-3, T-bet and TGF-ß may theoretically induce IL-4/A, IFN-γ and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ß were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-γ, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ß genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-γ, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ß in the muscles of fish injected with TGF-ß-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ß on both bacterial infections were negligible. Text Atlantic salmon MDPI Open Access Publishing Gata ENVELOPE(-19.702,-19.702,63.540,63.540) Biology 9 4 82 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
overexpression T-bet GATA-3 TGF-ß Atlantic salmon gene expression |
spellingShingle |
overexpression T-bet GATA-3 TGF-ß Atlantic salmon gene expression Tiril H. Slettjord Hege J. Sekkenes Heng Chi Jarl Bøgwald Trilochan Swain Roy A. Dalmo Jaya Kumari Swain Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon |
topic_facet |
overexpression T-bet GATA-3 TGF-ß Atlantic salmon gene expression |
description |
The overexpression of GATA-3, T-bet and TGF-ß may theoretically induce IL-4/A, IFN-γ and IL-17A expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged T-bet, GATA-3 and TGF-ß were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of IFN-γ, IL-4/13A and IL-17A. In addition, the fish were either experimentally challenged with Vibrio anguillarum (VA group) or Piscirickettsia salmonis (PS group). The reporter gene (RFP) inserted upstream of the GATA-3, T-bet and TGF-ß genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of GATA-3 and T-bet-encoding plasmids, the expression of GATA-3 and T-bet was high at the injection site. The spleen expression of IFN-γ, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The T-bet and GATA-3-overexpressing fish expressed high T-bet and GATA-3 mRNA levels in the muscles and on day 4 post-challenge. The expression of TGF-ß in the muscles of fish injected with TGF-ß-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of T-bet, GATA-3 and TGF-ß on both bacterial infections were negligible. |
format |
Text |
author |
Tiril H. Slettjord Hege J. Sekkenes Heng Chi Jarl Bøgwald Trilochan Swain Roy A. Dalmo Jaya Kumari Swain |
author_facet |
Tiril H. Slettjord Hege J. Sekkenes Heng Chi Jarl Bøgwald Trilochan Swain Roy A. Dalmo Jaya Kumari Swain |
author_sort |
Tiril H. Slettjord |
title |
Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon |
title_short |
Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon |
title_full |
Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon |
title_fullStr |
Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon |
title_full_unstemmed |
Overexpression of T-bet, GATA-3 and TGF-ß Induces IFN-γ, IL-4/13A, and IL-17A Expression in Atlantic Salmon |
title_sort |
overexpression of t-bet, gata-3 and tgf-ß induces ifn-γ, il-4/13a, and il-17a expression in atlantic salmon |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/biology9040082 |
op_coverage |
agris |
long_lat |
ENVELOPE(-19.702,-19.702,63.540,63.540) |
geographic |
Gata |
geographic_facet |
Gata |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Biology; Volume 9; Issue 4; Pages: 82 |
op_relation |
Immunology https://dx.doi.org/10.3390/biology9040082 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology9040082 |
container_title |
Biology |
container_volume |
9 |
container_issue |
4 |
container_start_page |
82 |
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1774715801406799872 |