Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis
The present study analyses the interactions between Philasterides dicentrarchi (a ciliate parasite that causes high mortalities in cultured flatfish) and the peritoneal cells of the turbot Scophthalmus maximus during an experimental infection. The transcriptomic response was evaluated in the parasit...
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ftmdpi:oai:mdpi.com:/2079-7737/9/10/337/ 2023-08-20T04:09:40+02:00 Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis Alejandra Valle José Manuel Leiro Patricia Pereiro Antonio Figueras Beatriz Novoa Ron P. H. Dirks Jesús Lamas agris 2020-10-15 application/pdf https://doi.org/10.3390/biology9100337 EN eng Multidisciplinary Digital Publishing Institute Genetics and Genomics https://dx.doi.org/10.3390/biology9100337 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 9; Issue 10; Pages: 337 Philasterides dicentrarchi turbot immune response transcriptomics infection Text 2020 ftmdpi https://doi.org/10.3390/biology9100337 2023-08-01T00:16:44Z The present study analyses the interactions between Philasterides dicentrarchi (a ciliate parasite that causes high mortalities in cultured flatfish) and the peritoneal cells of the turbot Scophthalmus maximus during an experimental infection. The transcriptomic response was evaluated in the parasites and in the fish peritoneal cells, at 1, 2 and 4 h post-infection (hpi) in turbot injected intraperitoneally (ip) with 107 ciliates and at 12 and 48 hpi in turbot injected ip with 105 ciliates. Numerous genes were differentially expressed (DE) in P. dicentrarchi, relative to their expression in control ciliates (0 hpi): 407 (369 were up-regulated) at 1 hpi, 769 (415 were up-regulated) at 2 hpi and 507 (119 were up-regulated) at 4 hpi. Gene ontology (GO) analysis of the DE genes showed that the most representative categories of biological processes affected at 1, 2 and 4 hpi were biosynthetic processes, catabolic processes, biogenesis, proteolysis and transmembrane transport. Twelve genes of the ABC transporter family and eight genes of the leishmanolysin family were DE at 1, 2 and 4 hpi. Most of these genes were strongly up-regulated (UR), suggesting that they are involved in P. dicentrarchi infection. A third group of UR genes included several genes related to ribosome biogenesis, DNA transcription and RNA translation. However, expression of tubulins and tubulin associated proteins, such as kinesins or dyneins, which play key roles in ciliate division and movement, was down-regulated (DR). Similarly, genes that coded for lysosomal proteins or that participate in the cell cycle mitotic control, glycolysis, the Krebs cycle and/or in the electron transport chain were also DR. The transcriptomic analysis also revealed that in contrast to many parasites, which passively evade the host immune system, P. dicentrarchi strongly stimulated turbot peritoneal cells. Many genes related to inflammation were DE in peritoneal cells at 1, 2 and 4 hpi. However, the response was much lower at 12 hpi and almost disappeared completely ... Text Scophthalmus maximus Turbot MDPI Open Access Publishing Krebs ENVELOPE(-61.467,-61.467,-64.633,-64.633) Biology 9 10 337 |
institution |
Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Philasterides dicentrarchi turbot immune response transcriptomics infection |
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Philasterides dicentrarchi turbot immune response transcriptomics infection Alejandra Valle José Manuel Leiro Patricia Pereiro Antonio Figueras Beatriz Novoa Ron P. H. Dirks Jesús Lamas Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis |
topic_facet |
Philasterides dicentrarchi turbot immune response transcriptomics infection |
description |
The present study analyses the interactions between Philasterides dicentrarchi (a ciliate parasite that causes high mortalities in cultured flatfish) and the peritoneal cells of the turbot Scophthalmus maximus during an experimental infection. The transcriptomic response was evaluated in the parasites and in the fish peritoneal cells, at 1, 2 and 4 h post-infection (hpi) in turbot injected intraperitoneally (ip) with 107 ciliates and at 12 and 48 hpi in turbot injected ip with 105 ciliates. Numerous genes were differentially expressed (DE) in P. dicentrarchi, relative to their expression in control ciliates (0 hpi): 407 (369 were up-regulated) at 1 hpi, 769 (415 were up-regulated) at 2 hpi and 507 (119 were up-regulated) at 4 hpi. Gene ontology (GO) analysis of the DE genes showed that the most representative categories of biological processes affected at 1, 2 and 4 hpi were biosynthetic processes, catabolic processes, biogenesis, proteolysis and transmembrane transport. Twelve genes of the ABC transporter family and eight genes of the leishmanolysin family were DE at 1, 2 and 4 hpi. Most of these genes were strongly up-regulated (UR), suggesting that they are involved in P. dicentrarchi infection. A third group of UR genes included several genes related to ribosome biogenesis, DNA transcription and RNA translation. However, expression of tubulins and tubulin associated proteins, such as kinesins or dyneins, which play key roles in ciliate division and movement, was down-regulated (DR). Similarly, genes that coded for lysosomal proteins or that participate in the cell cycle mitotic control, glycolysis, the Krebs cycle and/or in the electron transport chain were also DR. The transcriptomic analysis also revealed that in contrast to many parasites, which passively evade the host immune system, P. dicentrarchi strongly stimulated turbot peritoneal cells. Many genes related to inflammation were DE in peritoneal cells at 1, 2 and 4 hpi. However, the response was much lower at 12 hpi and almost disappeared completely ... |
format |
Text |
author |
Alejandra Valle José Manuel Leiro Patricia Pereiro Antonio Figueras Beatriz Novoa Ron P. H. Dirks Jesús Lamas |
author_facet |
Alejandra Valle José Manuel Leiro Patricia Pereiro Antonio Figueras Beatriz Novoa Ron P. H. Dirks Jesús Lamas |
author_sort |
Alejandra Valle |
title |
Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis |
title_short |
Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis |
title_full |
Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis |
title_fullStr |
Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis |
title_full_unstemmed |
Interactions between the Parasite Philasterides dicentrarchi and the Immune System of the Turbot Scophthalmus maximus. A Transcriptomic Analysis |
title_sort |
interactions between the parasite philasterides dicentrarchi and the immune system of the turbot scophthalmus maximus. a transcriptomic analysis |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/biology9100337 |
op_coverage |
agris |
long_lat |
ENVELOPE(-61.467,-61.467,-64.633,-64.633) |
geographic |
Krebs |
geographic_facet |
Krebs |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Biology; Volume 9; Issue 10; Pages: 337 |
op_relation |
Genetics and Genomics https://dx.doi.org/10.3390/biology9100337 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology9100337 |
container_title |
Biology |
container_volume |
9 |
container_issue |
10 |
container_start_page |
337 |
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