Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula
We studied the fungal diversity present in soils sampled along a deglaciated chronosequence from para- to periglacial conditions on James Ross Island, north-east Antarctic Peninsula, using DNA metabarcoding. A total of 88 amplicon sequence variants (ASVs) were detected, dominated by the phyla Ascomy...
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ftmdpi:oai:mdpi.com:/2079-7737/12/2/275/ 2023-08-20T04:01:08+02:00 Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula Vivian N. Gonçalves Juan M. Lirio Silvia H. Coria Fabyano A. C. Lopes Peter Convey Fábio S. de Oliveira Micheline Carvalho-Silva Paulo E. A. S. Câmara Luiz H. Rosa agris 2023-02-09 application/pdf https://doi.org/10.3390/biology12020275 EN eng Multidisciplinary Digital Publishing Institute Ecology https://dx.doi.org/10.3390/biology12020275 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 12; Issue 2; Pages: 275 Antarctica extremophiles environmental DNA fungi metabarcoding taxonomy Text 2023 ftmdpi https://doi.org/10.3390/biology12020275 2023-08-01T08:43:26Z We studied the fungal diversity present in soils sampled along a deglaciated chronosequence from para- to periglacial conditions on James Ross Island, north-east Antarctic Peninsula, using DNA metabarcoding. A total of 88 amplicon sequence variants (ASVs) were detected, dominated by the phyla Ascomycota, Basidiomycota and Mortierellomycota. The uncommon phyla Chytridiomycota, Rozellomycota, Monoblepharomycota, Zoopagomycota and Basidiobolomycota were detected. Unknown fungi identified at higher hierarchical taxonomic levels (Fungal sp. 1, Fungal sp. 2, Spizellomycetales sp. and Rozellomycotina sp.) and taxa identified at generic and specific levels (Mortierella sp., Pseudogymnoascus sp., Mortierella alpina, M. turficola, Neoascochyta paspali, Penicillium sp. and Betamyces sp.) dominated the assemblages. In general, the assemblages displayed high diversity and richness, and moderate dominance. Only 12 of the fungal ASVs were detected in all chronosequence soils sampled. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected. Based on the sequence diversity obtained, Clearwater Mesa soils contain a complex fungal community, including the presence of fungal groups generally considered rare in Antarctica, with dominant taxa recognized as cold-adapted cosmopolitan, endemic, saprotrophic and phytopathogenic fungi. Clearwater Mesa ecosystems are impacted by the effects of regional climatic changes, and may provide a natural observatory to understand climate change effects over time. Text Antarc* Antarctic Antarctic Peninsula Antarctica James Ross Island Ross Island MDPI Open Access Publishing Antarctic Antarctic Peninsula Clearwater Mesa ENVELOPE(-57.717,-57.717,-64.025,-64.025) Ross Island Biology 12 2 275 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctica extremophiles environmental DNA fungi metabarcoding taxonomy |
spellingShingle |
Antarctica extremophiles environmental DNA fungi metabarcoding taxonomy Vivian N. Gonçalves Juan M. Lirio Silvia H. Coria Fabyano A. C. Lopes Peter Convey Fábio S. de Oliveira Micheline Carvalho-Silva Paulo E. A. S. Câmara Luiz H. Rosa Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula |
topic_facet |
Antarctica extremophiles environmental DNA fungi metabarcoding taxonomy |
description |
We studied the fungal diversity present in soils sampled along a deglaciated chronosequence from para- to periglacial conditions on James Ross Island, north-east Antarctic Peninsula, using DNA metabarcoding. A total of 88 amplicon sequence variants (ASVs) were detected, dominated by the phyla Ascomycota, Basidiomycota and Mortierellomycota. The uncommon phyla Chytridiomycota, Rozellomycota, Monoblepharomycota, Zoopagomycota and Basidiobolomycota were detected. Unknown fungi identified at higher hierarchical taxonomic levels (Fungal sp. 1, Fungal sp. 2, Spizellomycetales sp. and Rozellomycotina sp.) and taxa identified at generic and specific levels (Mortierella sp., Pseudogymnoascus sp., Mortierella alpina, M. turficola, Neoascochyta paspali, Penicillium sp. and Betamyces sp.) dominated the assemblages. In general, the assemblages displayed high diversity and richness, and moderate dominance. Only 12 of the fungal ASVs were detected in all chronosequence soils sampled. Sequences representing saprophytic, pathogenic and symbiotic fungi were detected. Based on the sequence diversity obtained, Clearwater Mesa soils contain a complex fungal community, including the presence of fungal groups generally considered rare in Antarctica, with dominant taxa recognized as cold-adapted cosmopolitan, endemic, saprotrophic and phytopathogenic fungi. Clearwater Mesa ecosystems are impacted by the effects of regional climatic changes, and may provide a natural observatory to understand climate change effects over time. |
format |
Text |
author |
Vivian N. Gonçalves Juan M. Lirio Silvia H. Coria Fabyano A. C. Lopes Peter Convey Fábio S. de Oliveira Micheline Carvalho-Silva Paulo E. A. S. Câmara Luiz H. Rosa |
author_facet |
Vivian N. Gonçalves Juan M. Lirio Silvia H. Coria Fabyano A. C. Lopes Peter Convey Fábio S. de Oliveira Micheline Carvalho-Silva Paulo E. A. S. Câmara Luiz H. Rosa |
author_sort |
Vivian N. Gonçalves |
title |
Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula |
title_short |
Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula |
title_full |
Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula |
title_fullStr |
Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula |
title_full_unstemmed |
Soil Fungal Diversity and Ecology Assessed Using DNA Metabarcoding along a Deglaciated Chronosequence at Clearwater Mesa, James Ross Island, Antarctic Peninsula |
title_sort |
soil fungal diversity and ecology assessed using dna metabarcoding along a deglaciated chronosequence at clearwater mesa, james ross island, antarctic peninsula |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/biology12020275 |
op_coverage |
agris |
long_lat |
ENVELOPE(-57.717,-57.717,-64.025,-64.025) |
geographic |
Antarctic Antarctic Peninsula Clearwater Mesa Ross Island |
geographic_facet |
Antarctic Antarctic Peninsula Clearwater Mesa Ross Island |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica James Ross Island Ross Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica James Ross Island Ross Island |
op_source |
Biology; Volume 12; Issue 2; Pages: 275 |
op_relation |
Ecology https://dx.doi.org/10.3390/biology12020275 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology12020275 |
container_title |
Biology |
container_volume |
12 |
container_issue |
2 |
container_start_page |
275 |
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1774722970195853312 |