Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica)
In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. W...
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ftmdpi:oai:mdpi.com:/2079-7737/11/8/1143/ 2023-08-20T04:01:05+02:00 Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval agris 2022-07-29 application/pdf https://doi.org/10.3390/biology11081143 EN eng Multidisciplinary Digital Publishing Institute Microbiology https://dx.doi.org/10.3390/biology11081143 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 11; Issue 8; Pages: 1143 meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity antibiotic resistance Text 2022 ftmdpi https://doi.org/10.3390/biology11081143 2023-08-01T05:53:59Z In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86–94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera Pseudomonas, Shewanella, Acinetobacter, Sporosarcina, Facklamia, Carnobacterium, Arthrobacter, Brachybacterium, Micrococcus, Agrococcus, Leifsonia, and Flavobacterium. Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance. Text Antarc* Antarctic Antarctica East Antarctica Enderby Land MDPI Open Access Publishing Antarctic East Antarctica Thala Hills ENVELOPE(46.000,46.000,-67.667,-67.667) Biology 11 8 1143 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity antibiotic resistance |
spellingShingle |
meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity antibiotic resistance Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
topic_facet |
meltwater ponds Antarctic bacteria 16S rRNA gene sequencing plate-based assays thermotolerance enzymatic activity antibiotic resistance |
description |
In this study, for the first time, we report the identification and characterization of culturable fast-growing bacteria isolated from the sea-affected temporary meltwater ponds (MPs) in the East Antarctica area of the Vecherny region (−67.656317, 46.175058) of the Thala Hills Oasis, Enderby Land. Water samples from the studied MPs showed alkaline pH (from 8.0 to 10.1) and highly varied total dissolved solids (86–94,000 mg/L). In total, twenty-nine bacterial isolates were retrieved from the studied MPs. The phylogenetic analysis based on 16S rRNA gene sequence similarities showed that the isolated bacteria belong to the phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes and the twelve genera Pseudomonas, Shewanella, Acinetobacter, Sporosarcina, Facklamia, Carnobacterium, Arthrobacter, Brachybacterium, Micrococcus, Agrococcus, Leifsonia, and Flavobacterium. Most of the isolated bacteria were psychrotrophs and showed the production of one or more extracellular enzymes. Lipolytic and proteolytic activities were more prevalent among the isolates. Five isolates from the Actinobacteria phylum and one isolate from the Bacteroidetes phylum had strong pigmentation. Antibiotic susceptibility testing revealed that most of the isolates are resistant to at least one antibiotic, and seven isolates showed multi-resistance. |
format |
Text |
author |
Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval |
author_facet |
Volha Akulava Uladzislau Miamin Katsiaryna Akhremchuk Leonid Valentovich Andrey Dolgikh Volha Shapaval |
author_sort |
Volha Akulava |
title |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_short |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_full |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_fullStr |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_full_unstemmed |
Isolation, Physiological Characterization, and Antibiotic Susceptibility Testing of Fast-Growing Bacteria from the Sea-Affected Temporary Meltwater Ponds in the Thala Hills Oasis (Enderby Land, East Antarctica) |
title_sort |
isolation, physiological characterization, and antibiotic susceptibility testing of fast-growing bacteria from the sea-affected temporary meltwater ponds in the thala hills oasis (enderby land, east antarctica) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/biology11081143 |
op_coverage |
agris |
long_lat |
ENVELOPE(46.000,46.000,-67.667,-67.667) |
geographic |
Antarctic East Antarctica Thala Hills |
geographic_facet |
Antarctic East Antarctica Thala Hills |
genre |
Antarc* Antarctic Antarctica East Antarctica Enderby Land |
genre_facet |
Antarc* Antarctic Antarctica East Antarctica Enderby Land |
op_source |
Biology; Volume 11; Issue 8; Pages: 1143 |
op_relation |
Microbiology https://dx.doi.org/10.3390/biology11081143 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology11081143 |
container_title |
Biology |
container_volume |
11 |
container_issue |
8 |
container_start_page |
1143 |
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1774722533936857088 |