Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania
Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles (Clethrionomysglareolus) captured in several locations in Lithuania were examined in the present study. Based on morpho...
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ftmdpi:oai:mdpi.com:/2079-7737/11/4/512/ 2023-08-20T04:09:25+02:00 Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas agris 2022-03-26 application/pdf https://doi.org/10.3390/biology11040512 EN eng Multidisciplinary Digital Publishing Institute Evolutionary Biology https://dx.doi.org/10.3390/biology11040512 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 11; Issue 4; Pages: 512 bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny Text 2022 ftmdpi https://doi.org/10.3390/biology11040512 2023-08-01T04:34:41Z Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles (Clethrionomysglareolus) captured in several locations in Lithuania were examined in the present study. Based on morphological, genetic, and phylogenetic results, sarcocysts detected in one bank vole were described as Sarcocystis myodes n. sp. Using light microscopy analysis, the observed sarcocysts were ribbon-shaped, 6000–3000 × 70–220 µm in size. Sarcocysts were characterized by a relatively thin (about 1 μm) and apparently smooth cyst wall. The lancet-shaped bradyzoites were 9.6–12.0 × 3.1–4.6 μm in size. By transmission electron microscopy, the sarcocyst wall was up to 1 μm thick, parasitophorous vacuolar membrane had small knob-like blebs. Based on 18S rDNA, 28S rDNA, cox1, rpoB, and ITS1 loci, S. myodes showed highest similarity with S. ratti from the black rat (Rattus rattus). According to phylogenetic placement, S. myodes was most closely related to Sarcocystis spp. that employ predatory mammals as their definitive hosts. Morphologically, sarcocysts of S. myodes have similar features to those of S. cernae, S. dirumpens, and S. montanaensis described in voles, however, they use birds of prey or snakes as their definitive hosts. Text Rattus rattus MDPI Open Access Publishing Biology 11 4 512 |
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ftmdpi |
language |
English |
topic |
bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny |
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bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania |
topic_facet |
bank vole Sarcocystis myodes species description microscopy molecular characterization phylogeny |
description |
Numerous rodent species have been broadly examined for Sarcocystis parasites. Nevertheless, recent investigations on Sarcocystis spp. in voles are lacking. As many as 45 bank voles (Clethrionomysglareolus) captured in several locations in Lithuania were examined in the present study. Based on morphological, genetic, and phylogenetic results, sarcocysts detected in one bank vole were described as Sarcocystis myodes n. sp. Using light microscopy analysis, the observed sarcocysts were ribbon-shaped, 6000–3000 × 70–220 µm in size. Sarcocysts were characterized by a relatively thin (about 1 μm) and apparently smooth cyst wall. The lancet-shaped bradyzoites were 9.6–12.0 × 3.1–4.6 μm in size. By transmission electron microscopy, the sarcocyst wall was up to 1 μm thick, parasitophorous vacuolar membrane had small knob-like blebs. Based on 18S rDNA, 28S rDNA, cox1, rpoB, and ITS1 loci, S. myodes showed highest similarity with S. ratti from the black rat (Rattus rattus). According to phylogenetic placement, S. myodes was most closely related to Sarcocystis spp. that employ predatory mammals as their definitive hosts. Morphologically, sarcocysts of S. myodes have similar features to those of S. cernae, S. dirumpens, and S. montanaensis described in voles, however, they use birds of prey or snakes as their definitive hosts. |
format |
Text |
author |
Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas |
author_facet |
Eglė Rudaitytė-Lukošienė Marius Jasiulionis Linas Balčiauskas Petras Prakas Vitalijus Stirkė Dalius Butkauskas |
author_sort |
Eglė Rudaitytė-Lukošienė |
title |
Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania |
title_short |
Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania |
title_full |
Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania |
title_fullStr |
Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania |
title_full_unstemmed |
Morphological and Molecular Description of Sarcocystismyodes n. sp. from the Bank Vole (Clethrionomys glareolus) in Lithuania |
title_sort |
morphological and molecular description of sarcocystismyodes n. sp. from the bank vole (clethrionomys glareolus) in lithuania |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/biology11040512 |
op_coverage |
agris |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
Biology; Volume 11; Issue 4; Pages: 512 |
op_relation |
Evolutionary Biology https://dx.doi.org/10.3390/biology11040512 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology11040512 |
container_title |
Biology |
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11 |
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4 |
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512 |
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