Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment
Regarding the sperm of cold-water fish, the contributions of different bioenergetic pathways, including mitochondrial respiration, to energy production at the spawning temperature and its adaptation at the maximum critical temperature (CTmax) are unclear. The roles of glycolysis, fatty acid oxidatio...
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ftmdpi:oai:mdpi.com:/2079-7737/10/8/739/ 2023-08-20T04:05:41+02:00 Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment Deepali Rahi Borys Dzyuba Tomas Policar Oleksandr Malinovskyi Marek Rodina Viktoriya Dzyuba agris 2021-08-01 application/pdf https://doi.org/10.3390/biology10080739 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/biology10080739 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 10; Issue 8; Pages: 739 oxidative phosphorylation glycolysis fatty acid oxidation sperm motility spawning temperature maximum critical temperature cold-water fish Text 2021 ftmdpi https://doi.org/10.3390/biology10080739 2023-08-01T02:20:30Z Regarding the sperm of cold-water fish, the contributions of different bioenergetic pathways, including mitochondrial respiration, to energy production at the spawning temperature and its adaptation at the maximum critical temperature (CTmax) are unclear. The roles of glycolysis, fatty acid oxidation, oxidative phosphorylation (OXPHOS) at 4 °C, and OXPHOS at 15 °C for energy production in burbot (Lota lota) spermatozoa were studied by motility and the oxygen consumption rate (OCR) (with and without pathway inhibitors and the OXPHOS uncoupler). At both temperatures, the effects of the inhibitors and the uncoupler on the motility duration, curvilinear velocity, and track linearity were insignificant; in addition, the OCRs in activation and non-activation media differed insignificantly and were not enhanced after uncoupler treatment. After inhibitor treatment in both media, OXPHOS was insignificantly different at the 2, 30, and 60 s time points at 4 °C but was reduced significantly at the 30 and 60 s time points after treatment with sodium azide at 15 °C. In conclusion, for burbot sperm at both the spawning temperature and the CTmax, the energy synthesized via OXPHOS during motility was insufficient. Therefore, the majority of the energy required to sustain motility was derived from pre-accumulated energy produced and stored during the quiescent state of the spermatozoa. Text Burbot Lota lota lota MDPI Open Access Publishing Biology 10 8 739 |
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oxidative phosphorylation glycolysis fatty acid oxidation sperm motility spawning temperature maximum critical temperature cold-water fish |
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oxidative phosphorylation glycolysis fatty acid oxidation sperm motility spawning temperature maximum critical temperature cold-water fish Deepali Rahi Borys Dzyuba Tomas Policar Oleksandr Malinovskyi Marek Rodina Viktoriya Dzyuba Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment |
topic_facet |
oxidative phosphorylation glycolysis fatty acid oxidation sperm motility spawning temperature maximum critical temperature cold-water fish |
description |
Regarding the sperm of cold-water fish, the contributions of different bioenergetic pathways, including mitochondrial respiration, to energy production at the spawning temperature and its adaptation at the maximum critical temperature (CTmax) are unclear. The roles of glycolysis, fatty acid oxidation, oxidative phosphorylation (OXPHOS) at 4 °C, and OXPHOS at 15 °C for energy production in burbot (Lota lota) spermatozoa were studied by motility and the oxygen consumption rate (OCR) (with and without pathway inhibitors and the OXPHOS uncoupler). At both temperatures, the effects of the inhibitors and the uncoupler on the motility duration, curvilinear velocity, and track linearity were insignificant; in addition, the OCRs in activation and non-activation media differed insignificantly and were not enhanced after uncoupler treatment. After inhibitor treatment in both media, OXPHOS was insignificantly different at the 2, 30, and 60 s time points at 4 °C but was reduced significantly at the 30 and 60 s time points after treatment with sodium azide at 15 °C. In conclusion, for burbot sperm at both the spawning temperature and the CTmax, the energy synthesized via OXPHOS during motility was insufficient. Therefore, the majority of the energy required to sustain motility was derived from pre-accumulated energy produced and stored during the quiescent state of the spermatozoa. |
format |
Text |
author |
Deepali Rahi Borys Dzyuba Tomas Policar Oleksandr Malinovskyi Marek Rodina Viktoriya Dzyuba |
author_facet |
Deepali Rahi Borys Dzyuba Tomas Policar Oleksandr Malinovskyi Marek Rodina Viktoriya Dzyuba |
author_sort |
Deepali Rahi |
title |
Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment |
title_short |
Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment |
title_full |
Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment |
title_fullStr |
Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment |
title_full_unstemmed |
Bioenergetic Pathways in the Sperm of an Under-Ice Spawning Fish, Burbot (Lota lota): The Role of Mitochondrial Respiration in a Varying Thermal Environment |
title_sort |
bioenergetic pathways in the sperm of an under-ice spawning fish, burbot (lota lota): the role of mitochondrial respiration in a varying thermal environment |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/biology10080739 |
op_coverage |
agris |
genre |
Burbot Lota lota lota |
genre_facet |
Burbot Lota lota lota |
op_source |
Biology; Volume 10; Issue 8; Pages: 739 |
op_relation |
https://dx.doi.org/10.3390/biology10080739 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology10080739 |
container_title |
Biology |
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10 |
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8 |
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739 |
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