Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica
Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil o...
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ftmdpi:oai:mdpi.com:/2079-7737/10/6/493/ 2023-08-20T04:00:48+02:00 Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica Ahmad Fareez Ahmad Roslee Claudio Gomez-Fuentes Nur Nadhirah Zakaria Nor Azmi Shaharuddin Azham Zulkharnain Khalilah Abdul Khalil Peter Convey Siti Aqlima Ahmad agris 2021-06-02 application/pdf https://doi.org/10.3390/biology10060493 EN eng Multidisciplinary Digital Publishing Institute Biotechnology https://dx.doi.org/10.3390/biology10060493 https://creativecommons.org/licenses/by/4.0/ Biology; Volume 10; Issue 6; Pages: 493 diesel microbial consortium biodegradation response-surface methodology (RSM) kinetic model Text 2021 ftmdpi https://doi.org/10.3390/biology10060493 2023-08-01T01:51:52Z Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil on the north-west Antarctic Peninsula, to metabolise diesel fuel as the sole carbon source in a shake-flask setting. Factors expected to influence the efficiency of diesel biodegradation, namely temperature, initial diesel concentration, nitrogen source type and concentration, salinity and pH were studied. Consortium BS24 displayed optimal cell growth and diesel degradation activity at 1.0% NaCl, pH 7.5, 0.5 g/L NH4Cl and 2.0% v/v initial diesel concentration during one-factor-at-a-time (OFAT) analyses. The consortium was psychrotolerant based on the optimum growth temperature of 10‒15 °C. In conventionally optimised media, the highest total petroleum hydrocarbons (TPH) mineralisation was 85% over a 7-day incubation. Further optimisation of conditions predicted through statistical response-surface methodology (RSM) (1.0% NaCl, pH 7.25, 0.75 g/L NH4Cl, 12.5 °C and 1.75% v/v initial diesel concentration) boosted mineralisation to 95% over a 7-day incubation. A Tessier secondary model best described the growth pattern of BS24 in diesel-enriched medium, with maximum specific growth rate, μmax, substrate inhibition constant, Ki and half saturation constant, Ks, being 0.9996 h−1, 1.356% v/v and 1.238% v/v, respectively. The data obtained suggest the potential of microbial consortia such as BS24 in bioremediation applications in low-temperature diesel-polluted soils. Text Antarc* Antarctic Antarctic Peninsula Antarctica MDPI Open Access Publishing Antarctic Antarctic Peninsula Trinity Peninsula ENVELOPE(-58.000,-58.000,-63.500,-63.500) Biology 10 6 493 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
diesel microbial consortium biodegradation response-surface methodology (RSM) kinetic model |
spellingShingle |
diesel microbial consortium biodegradation response-surface methodology (RSM) kinetic model Ahmad Fareez Ahmad Roslee Claudio Gomez-Fuentes Nur Nadhirah Zakaria Nor Azmi Shaharuddin Azham Zulkharnain Khalilah Abdul Khalil Peter Convey Siti Aqlima Ahmad Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica |
topic_facet |
diesel microbial consortium biodegradation response-surface methodology (RSM) kinetic model |
description |
Pollution associated with petrogenic hydrocarbons is increasing in Antarctica due to a combination of increasing human activity and the continent’s unforgiving environmental conditions. The current study focuses on the ability of a cold-adapted crude microbial consortium (BS24), isolated from soil on the north-west Antarctic Peninsula, to metabolise diesel fuel as the sole carbon source in a shake-flask setting. Factors expected to influence the efficiency of diesel biodegradation, namely temperature, initial diesel concentration, nitrogen source type and concentration, salinity and pH were studied. Consortium BS24 displayed optimal cell growth and diesel degradation activity at 1.0% NaCl, pH 7.5, 0.5 g/L NH4Cl and 2.0% v/v initial diesel concentration during one-factor-at-a-time (OFAT) analyses. The consortium was psychrotolerant based on the optimum growth temperature of 10‒15 °C. In conventionally optimised media, the highest total petroleum hydrocarbons (TPH) mineralisation was 85% over a 7-day incubation. Further optimisation of conditions predicted through statistical response-surface methodology (RSM) (1.0% NaCl, pH 7.25, 0.75 g/L NH4Cl, 12.5 °C and 1.75% v/v initial diesel concentration) boosted mineralisation to 95% over a 7-day incubation. A Tessier secondary model best described the growth pattern of BS24 in diesel-enriched medium, with maximum specific growth rate, μmax, substrate inhibition constant, Ki and half saturation constant, Ks, being 0.9996 h−1, 1.356% v/v and 1.238% v/v, respectively. The data obtained suggest the potential of microbial consortia such as BS24 in bioremediation applications in low-temperature diesel-polluted soils. |
format |
Text |
author |
Ahmad Fareez Ahmad Roslee Claudio Gomez-Fuentes Nur Nadhirah Zakaria Nor Azmi Shaharuddin Azham Zulkharnain Khalilah Abdul Khalil Peter Convey Siti Aqlima Ahmad |
author_facet |
Ahmad Fareez Ahmad Roslee Claudio Gomez-Fuentes Nur Nadhirah Zakaria Nor Azmi Shaharuddin Azham Zulkharnain Khalilah Abdul Khalil Peter Convey Siti Aqlima Ahmad |
author_sort |
Ahmad Fareez Ahmad Roslee |
title |
Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica |
title_short |
Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica |
title_full |
Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica |
title_fullStr |
Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica |
title_full_unstemmed |
Growth Optimisation and Kinetic Profiling of Diesel Biodegradation by a Cold-Adapted Microbial Consortium Isolated from Trinity Peninsula, Antarctica |
title_sort |
growth optimisation and kinetic profiling of diesel biodegradation by a cold-adapted microbial consortium isolated from trinity peninsula, antarctica |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/biology10060493 |
op_coverage |
agris |
long_lat |
ENVELOPE(-58.000,-58.000,-63.500,-63.500) |
geographic |
Antarctic Antarctic Peninsula Trinity Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula Trinity Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica |
op_source |
Biology; Volume 10; Issue 6; Pages: 493 |
op_relation |
Biotechnology https://dx.doi.org/10.3390/biology10060493 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/biology10060493 |
container_title |
Biology |
container_volume |
10 |
container_issue |
6 |
container_start_page |
493 |
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1774720501064663040 |