Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a susta...
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ftmdpi:oai:mdpi.com:/2077-1312/9/6/648/ 2023-08-20T04:00:09+02:00 Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad agris 2021-06-11 application/pdf https://doi.org/10.3390/jmse9060648 EN eng Multidisciplinary Digital Publishing Institute Marine Biology https://dx.doi.org/10.3390/jmse9060648 https://creativecommons.org/licenses/by/4.0/ Journal of Marine Science and Engineering; Volume 9; Issue 6; Pages: 648 Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling Text 2021 ftmdpi https://doi.org/10.3390/jmse9060648 2023-08-01T01:55:48Z The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate (µmax) was 0.067 h−1. The data obtained support the potential use of marine bacteria in the bioremediation of Mo. Text Antarc* Antarctic Antarctica MDPI Open Access Publishing Antarctic Journal of Marine Science and Engineering 9 6 648 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling |
spellingShingle |
Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
topic_facet |
Antarctica molybdenum One-Factor-At-a-Time (OFAT) Response Surface Methodology (RSM) kinetic modelling |
description |
The extensive industrial use of the heavy metal molybdenum (Mo) has led to an emerging global pollution with its traces that can even be found in Antarctica. In response, a reduction process that transforms hexamolybdate (Mo6+) to a less toxic compound, Mo-blue, using microorganisms provides a sustainable remediation approach. The aim of this study was to investigate the reduction of Mo by a psychrotolerant Antarctic marine bacterium, Marinomonas sp. strain AQ5-A9. Mo reduction was optimised using One-Factor-At-a-Time (OFAT) and Response Surface Methodology (RSM). Subsequently, Mo reduction kinetics were further studied. OFAT results showed that maximum Mo reduction occurred in culture media conditions of pH 6.0 and 50 ppt salinity at 15 °C, with initial sucrose, nitrogen and molybdate concentrations of 2.0%, 3.0 g/L and 10 mM, respectively. Further optimization using RSM identified improved optimum conditions of pH 6.0 and 47 ppt salinity at 16 °C, with initial sucrose, nitrogen and molybdate concentrations of 1.8%, 2.25 g/L and 16 mM, respectively. Investigation of the kinetics of Mo reduction revealed Aiba as the best-fitting model. The calculated Aiba coefficient of maximum Mo reduction rate (µmax) was 0.067 h−1. The data obtained support the potential use of marine bacteria in the bioremediation of Mo. |
format |
Text |
author |
Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad |
author_facet |
Syazani Darham Khadijah Nabilah Mohd Zahri Azham Zulkharnain Suriana Sabri Claudio Gomez-Fuentes Peter Convey Khalilah Abdul Khalil Siti Aqlima Ahmad |
author_sort |
Syazani Darham |
title |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_short |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_full |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_fullStr |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_full_unstemmed |
Statistical Optimisation and Kinetic Studies of Molybdenum Reduction Using a Psychrotolerant Marine Bacteria Isolated from Antarctica |
title_sort |
statistical optimisation and kinetic studies of molybdenum reduction using a psychrotolerant marine bacteria isolated from antarctica |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/jmse9060648 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Journal of Marine Science and Engineering; Volume 9; Issue 6; Pages: 648 |
op_relation |
Marine Biology https://dx.doi.org/10.3390/jmse9060648 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/jmse9060648 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
9 |
container_issue |
6 |
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648 |
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1774716846905229312 |