Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii
Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii (PmFGF18) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domai...
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ftmdpi:oai:mdpi.com:/2077-1312/8/11/896/ 2023-08-20T04:06:04+02:00 Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang agris 2020-11-10 application/pdf https://doi.org/10.3390/jmse8110896 EN eng Multidisciplinary Digital Publishing Institute Marine Biology https://dx.doi.org/10.3390/jmse8110896 https://creativecommons.org/licenses/by/4.0/ Journal of Marine Science and Engineering; Volume 8; Issue 11; Pages: 896 FGF18 SNP growth traits Pinctada fucata martensii Text 2020 ftmdpi https://doi.org/10.3390/jmse8110896 2023-08-01T00:26:12Z Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii (PmFGF18) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domain analysis of PmFGF18 showed a distinctive FGF domain, with a high similarity to FGF18 protein sequences from Crassostrea gigas (43.35%) and C. virginica (37.43%). PmFGF18 expression was revealed in all analyzed tissues with a significantly higher expression level in the fast-growing group than the slow-growing group. The analysis of PmFGF18 polymorphism demonstrated 33 SNPs (single nucleotide polymorphisms) in the CDS and promoter region of PmFGF18 sequence. Association analysis revealed 19 SNPs (2 SNPs from CDS and 17 SNPs from the promoter region) associating significantly with growth traits. Among the associated SNPs, one SNP g.50918198 A > C was verified in the other breeding line. Therefore, PmFGF18 can be utilized as a candidate gene for growth, and its related SNPs could be used in selective breeding of P. f. martensii for the improvement of growth traits. Text Crassostrea gigas MDPI Open Access Publishing Journal of Marine Science and Engineering 8 11 896 |
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Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
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English |
topic |
FGF18 SNP growth traits Pinctada fucata martensii |
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FGF18 SNP growth traits Pinctada fucata martensii Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
topic_facet |
FGF18 SNP growth traits Pinctada fucata martensii |
description |
Fibroblast growth factor 18 (FGF18) plays an important functional role in skeletal growth and development. The FGF18 gene was characterized in pearl oyster Pinctada fucata martensii (PmFGF18) with the full-length sequence containing an open reading frame of 714 bp encoding 237 amino acids. The domain analysis of PmFGF18 showed a distinctive FGF domain, with a high similarity to FGF18 protein sequences from Crassostrea gigas (43.35%) and C. virginica (37.43%). PmFGF18 expression was revealed in all analyzed tissues with a significantly higher expression level in the fast-growing group than the slow-growing group. The analysis of PmFGF18 polymorphism demonstrated 33 SNPs (single nucleotide polymorphisms) in the CDS and promoter region of PmFGF18 sequence. Association analysis revealed 19 SNPs (2 SNPs from CDS and 17 SNPs from the promoter region) associating significantly with growth traits. Among the associated SNPs, one SNP g.50918198 A > C was verified in the other breeding line. Therefore, PmFGF18 can be utilized as a candidate gene for growth, and its related SNPs could be used in selective breeding of P. f. martensii for the improvement of growth traits. |
format |
Text |
author |
Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang |
author_facet |
Ruijuan Hao Chuchu Mo Linda Adzigbli Chuangye Yang Yuewen Deng Qingheng Wang |
author_sort |
Ruijuan Hao |
title |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_short |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_full |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_fullStr |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_full_unstemmed |
Molecular Cloning and Polymorphism Analysis of PmFGF18 from Pinctada fucata martensii |
title_sort |
molecular cloning and polymorphism analysis of pmfgf18 from pinctada fucata martensii |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/jmse8110896 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Journal of Marine Science and Engineering; Volume 8; Issue 11; Pages: 896 |
op_relation |
Marine Biology https://dx.doi.org/10.3390/jmse8110896 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/jmse8110896 |
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Journal of Marine Science and Engineering |
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8 |
container_issue |
11 |
container_start_page |
896 |
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1774716975730130944 |