Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov.
The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspe...
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ftmdpi:oai:mdpi.com:/2077-1312/10/11/1656/ 2023-08-20T04:01:07+02:00 Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. Riccardo Trentin Emanuela Moschin André Duarte Lopes Stefano Schiaparelli Luísa Custódio Isabella Moro agris 2022-11-04 application/pdf https://doi.org/10.3390/jmse10111656 EN eng Multidisciplinary Digital Publishing Institute Marine Environmental Science https://dx.doi.org/10.3390/jmse10111656 https://creativecommons.org/licenses/by/4.0/ Journal of Marine Science and Engineering; Volume 10; Issue 11; Pages: 1656 Antarctica biodiversity Ross Sea diatoms Craspedostauros Craspedostauros ineffabilis sp. nov. Craspedostauros zucchellii sp. nov. morphology molecular phylogeny chemical diversity Text 2022 ftmdpi https://doi.org/10.3390/jmse10111656 2023-08-01T07:11:42Z The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspedostauros: cribrate areolae, two “fore-and-aft” chloroplasts and a narrow “stauros”. This classification is congruent with the molecular phylogeny based on the concatenated 18S rDNA-rbcL-psbC alignment, which showed that these algae formed a monophyletic lineage including six taxonomically accepted species of Craspedostauros. Since the study of the evolution of this genus and of others raphe-bearing diatoms with a “stauros” is particularly challenging and their phylogeny is still debated, we tested alternative tree topologies to evaluate the relationships among these taxa. The metabolic fingerprinting approach was implemented for the assessment of the chemical diversity of IMA082A and IMA088A. In conclusion, combining (1) traditional morphological features used in diatoms identification, (2) phylogenetic analyses of the small subunit rDNA (18S rDNA), rbcL and psbC genes, and (3) metabolic fingerprint, we described the strains IMA082A and IMA088A as Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. as new species, respectively. Text Antarc* Antarctic Antarctica Ross Sea MDPI Open Access Publishing Antarctic Ross Sea Journal of Marine Science and Engineering 10 11 1656 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctica biodiversity Ross Sea diatoms Craspedostauros Craspedostauros ineffabilis sp. nov. Craspedostauros zucchellii sp. nov. morphology molecular phylogeny chemical diversity |
spellingShingle |
Antarctica biodiversity Ross Sea diatoms Craspedostauros Craspedostauros ineffabilis sp. nov. Craspedostauros zucchellii sp. nov. morphology molecular phylogeny chemical diversity Riccardo Trentin Emanuela Moschin André Duarte Lopes Stefano Schiaparelli Luísa Custódio Isabella Moro Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
topic_facet |
Antarctica biodiversity Ross Sea diatoms Craspedostauros Craspedostauros ineffabilis sp. nov. Craspedostauros zucchellii sp. nov. morphology molecular phylogeny chemical diversity |
description |
The current study focuses on the biological diversity of two strains of Antarctic diatoms (strains IMA082A and IMA088A) collected and isolated from the Ross Sea (Antarctica) during the XXXIV Italian Antarctic Expedition. Both species presented the typical morphological characters of the genus Craspedostauros: cribrate areolae, two “fore-and-aft” chloroplasts and a narrow “stauros”. This classification is congruent with the molecular phylogeny based on the concatenated 18S rDNA-rbcL-psbC alignment, which showed that these algae formed a monophyletic lineage including six taxonomically accepted species of Craspedostauros. Since the study of the evolution of this genus and of others raphe-bearing diatoms with a “stauros” is particularly challenging and their phylogeny is still debated, we tested alternative tree topologies to evaluate the relationships among these taxa. The metabolic fingerprinting approach was implemented for the assessment of the chemical diversity of IMA082A and IMA088A. In conclusion, combining (1) traditional morphological features used in diatoms identification, (2) phylogenetic analyses of the small subunit rDNA (18S rDNA), rbcL and psbC genes, and (3) metabolic fingerprint, we described the strains IMA082A and IMA088A as Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. as new species, respectively. |
format |
Text |
author |
Riccardo Trentin Emanuela Moschin André Duarte Lopes Stefano Schiaparelli Luísa Custódio Isabella Moro |
author_facet |
Riccardo Trentin Emanuela Moschin André Duarte Lopes Stefano Schiaparelli Luísa Custódio Isabella Moro |
author_sort |
Riccardo Trentin |
title |
Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_short |
Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_full |
Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_fullStr |
Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_full_unstemmed |
Molecular, Morphological and Chemical Diversity of Two New Species of Antarctic Diatoms, Craspedostauros ineffabilis sp. nov. and Craspedostauros zucchellii sp. nov. |
title_sort |
molecular, morphological and chemical diversity of two new species of antarctic diatoms, craspedostauros ineffabilis sp. nov. and craspedostauros zucchellii sp. nov. |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/jmse10111656 |
op_coverage |
agris |
geographic |
Antarctic Ross Sea |
geographic_facet |
Antarctic Ross Sea |
genre |
Antarc* Antarctic Antarctica Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica Ross Sea |
op_source |
Journal of Marine Science and Engineering; Volume 10; Issue 11; Pages: 1656 |
op_relation |
Marine Environmental Science https://dx.doi.org/10.3390/jmse10111656 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/jmse10111656 |
container_title |
Journal of Marine Science and Engineering |
container_volume |
10 |
container_issue |
11 |
container_start_page |
1656 |
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1774722816283770880 |