Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases
Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In...
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ftmdpi:oai:mdpi.com:/2076-393X/9/4/361/ 2023-08-20T04:05:19+02:00 Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases Valentina Valenzuela-Muñoz Bárbara P. Benavente Antonio Casuso Yeny Leal Cristian Gallardo-Escárate 2021-04-09 application/pdf https://doi.org/10.3390/vaccines9040361 EN eng Multidisciplinary Digital Publishing Institute Veterinary Vaccines https://dx.doi.org/10.3390/vaccines9040361 https://creativecommons.org/licenses/by/4.0/ Vaccines; Volume 9; Issue 4; Pages: 361 iron transport chimeric protein chelating activity Salmo salar Aeromonas salmonicida Caligus rogercresseyi Piscirickettsia salmonis Text 2021 ftmdpi https://doi.org/10.3390/vaccines9040361 2023-08-01T01:27:48Z Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath®) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath® protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath® was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath® and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath® showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high modulation of ... Text Atlantic salmon Salmo salar MDPI Open Access Publishing Vaccines 9 4 361 |
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language |
English |
topic |
iron transport chimeric protein chelating activity Salmo salar Aeromonas salmonicida Caligus rogercresseyi Piscirickettsia salmonis |
spellingShingle |
iron transport chimeric protein chelating activity Salmo salar Aeromonas salmonicida Caligus rogercresseyi Piscirickettsia salmonis Valentina Valenzuela-Muñoz Bárbara P. Benavente Antonio Casuso Yeny Leal Cristian Gallardo-Escárate Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
topic_facet |
iron transport chimeric protein chelating activity Salmo salar Aeromonas salmonicida Caligus rogercresseyi Piscirickettsia salmonis |
description |
Infection processes displayed by pathogens require the acquisition of essential inorganic nutrients and trace elements from the host to survive and proliferate. Without a doubt, iron is a crucial trace metal for all living organisms and also a pivotal component in the host–parasite interactions. In particular, the host reduces the iron available to face the infectious disease, increasing iron transport proteins’ expression and activating the heme synthesis and degradation pathways. Moreover, recent findings have suggested that iron metabolism modulation in fish promotes the immune response by reducing cellular iron toxicity. We hypothesized that recombinant proteins related to iron metabolism could modulate the fish’s immune system through iron metabolism and iron-responsive genes. Here a chimeric iron transport protein (IPath®) was bioinformatically designed and then expressed in a recombinant bacterial system. The IPath® protein showed a significant chelating activity under in vitro conditions and biological activity. Taking this evidence, a vaccine candidate based on IPath® was evaluated in Atlantic salmon challenged with three different fish pathogens. Experimental trials were conducted using two fish groups: one immunized with IPath® and another injected with adjutant as the control group. After 400 accumulated thermal units (ATUs), two different infection trials were performed. In the first one, fish were infected with the bacterium Aeromonas salmonicida, and in a second trial, fish were exposed to the ectoparasite Caligus rogercresseyi and subsequently infected with the intracellular bacterium Piscirickettsia salmonis. Fish immunized with IPath® showed a significant delay in the mortality curve in response to A. salmonicida and P. salmonis infections. However, no significant differences between infected and control fish groups were observed at the end of the experiment. Notably, sea lice burden reduction was observed in vaccinated Atlantic salmon. Transcriptional analysis evidenced a high modulation of ... |
format |
Text |
author |
Valentina Valenzuela-Muñoz Bárbara P. Benavente Antonio Casuso Yeny Leal Cristian Gallardo-Escárate |
author_facet |
Valentina Valenzuela-Muñoz Bárbara P. Benavente Antonio Casuso Yeny Leal Cristian Gallardo-Escárate |
author_sort |
Valentina Valenzuela-Muñoz |
title |
Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_short |
Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_full |
Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_fullStr |
Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_full_unstemmed |
Chimeric Protein IPath® with Chelating Activity Improves Atlantic Salmon’s Immunity against Infectious Diseases |
title_sort |
chimeric protein ipath® with chelating activity improves atlantic salmon’s immunity against infectious diseases |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/vaccines9040361 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Vaccines; Volume 9; Issue 4; Pages: 361 |
op_relation |
Veterinary Vaccines https://dx.doi.org/10.3390/vaccines9040361 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/vaccines9040361 |
container_title |
Vaccines |
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9 |
container_issue |
4 |
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361 |
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1774715811105079296 |