Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtain...
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ftmdpi:oai:mdpi.com:/2076-3921/9/4/325/ 2023-08-20T04:02:34+02:00 Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature Evangelia Chatzidimitriou Paola Bisaccia Francesca Corrà Marco Bonato Paola Irato Laura Manuto Stefano Toppo Rigers Bakiu Gianfranco Santovito agris 2020-04-17 application/pdf https://doi.org/10.3390/antiox9040325 EN eng Multidisciplinary Digital Publishing Institute Antioxidant Enzyme Systems https://dx.doi.org/10.3390/antiox9040325 https://creativecommons.org/licenses/by/4.0/ Antioxidants; Volume 9; Issue 4; Pages: 325 Antarctica Chionodraco hamatus cold adaptation gene expression molecular evolution protein purification superoxide dismutase teleosts Text 2020 ftmdpi https://doi.org/10.3390/antiox9040325 2023-07-31T23:23:02Z In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the “unclock-like” behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress. Text Antarc* Antarctic Antarctica antartic* Icefish Ross Sea MDPI Open Access Publishing Antarctic The Antarctic Ross Sea Antioxidants 9 4 325 |
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MDPI Open Access Publishing |
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English |
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Antarctica Chionodraco hamatus cold adaptation gene expression molecular evolution protein purification superoxide dismutase teleosts |
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Antarctica Chionodraco hamatus cold adaptation gene expression molecular evolution protein purification superoxide dismutase teleosts Evangelia Chatzidimitriou Paola Bisaccia Francesca Corrà Marco Bonato Paola Irato Laura Manuto Stefano Toppo Rigers Bakiu Gianfranco Santovito Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
topic_facet |
Antarctica Chionodraco hamatus cold adaptation gene expression molecular evolution protein purification superoxide dismutase teleosts |
description |
In the present study, we describe the purification and molecular characterization of Cu,Zn superoxide dismutase (SOD) from Chionodraco hamatus, an Antarctic teleost widely distributed in many areas of the Ross Sea that plays a pivotal role in the Antarctic food chain. The primary sequence was obtained using biochemical and molecular biology approaches and compared with Cu,Zn SODs from other organisms. Multiple sequence alignment using the amino acid sequence revealed that Cu,Zn SOD showed considerable sequence similarity with its orthologues from various vertebrate species, but also some specific substitutions directly linked to cold adaptation. Phylogenetic analyses presented the monophyletic status of Antartic Teleostei among the Perciformes, confirming the erratic differentiation of these proteins and concurring with the theory of the “unclock-like” behavior of Cu,Zn SOD evolution. Expression of C. hamatus Cu,Zn SOD at both the mRNA and protein levels were analyzed in various tissues, highlighting the regulation of gene expression related to environmental stress conditions and also animal physiology. The data presented are the first on the antioxidant enzymes of a fish belonging to the Channichthyidae family and represent an important starting point in understanding the antioxidant systems of these organisms that are subject to constant risk of oxidative stress. |
format |
Text |
author |
Evangelia Chatzidimitriou Paola Bisaccia Francesca Corrà Marco Bonato Paola Irato Laura Manuto Stefano Toppo Rigers Bakiu Gianfranco Santovito |
author_facet |
Evangelia Chatzidimitriou Paola Bisaccia Francesca Corrà Marco Bonato Paola Irato Laura Manuto Stefano Toppo Rigers Bakiu Gianfranco Santovito |
author_sort |
Evangelia Chatzidimitriou |
title |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_short |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_full |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_fullStr |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_full_unstemmed |
Copper/Zinc Superoxide Dismutase from the Crocodile Icefish Chionodraco hamatus: Antioxidant Defense at Constant Sub-Zero Temperature |
title_sort |
copper/zinc superoxide dismutase from the crocodile icefish chionodraco hamatus: antioxidant defense at constant sub-zero temperature |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/antiox9040325 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic Ross Sea |
geographic_facet |
Antarctic The Antarctic Ross Sea |
genre |
Antarc* Antarctic Antarctica antartic* Icefish Ross Sea |
genre_facet |
Antarc* Antarctic Antarctica antartic* Icefish Ross Sea |
op_source |
Antioxidants; Volume 9; Issue 4; Pages: 325 |
op_relation |
Antioxidant Enzyme Systems https://dx.doi.org/10.3390/antiox9040325 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox9040325 |
container_title |
Antioxidants |
container_volume |
9 |
container_issue |
4 |
container_start_page |
325 |
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1774713083119271936 |