Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro
High-quality eggs are essential for the sustainability of commercial aquaculture production. Melatonin is a potent candidate for regulating the growth and maturation of oocytes. Therefore, research on the effect of melatonin on marine fish oocytes in vitro has been conducted. The present study succe...
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ftmdpi:oai:mdpi.com:/2076-3921/12/7/1389/ 2023-08-20T04:09:39+02:00 Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro Jiarong Zhang Feixia Li Xiaoyu Zhang Ting Xie Hongyu Qin Junxian Lv Yunhong Gao Mingyue Li Yuntao Gao Yudong Jia agris 2023-07-06 application/pdf https://doi.org/10.3390/antiox12071389 EN eng Multidisciplinary Digital Publishing Institute Health Outcomes of Antioxidants and Oxidative Stress https://dx.doi.org/10.3390/antiox12071389 https://creativecommons.org/licenses/by/4.0/ Antioxidants; Volume 12; Issue 7; Pages: 1389 Scophthalmus maximus oocyte culture and maturation melatonin antioxidative capacity apoptosis Text 2023 ftmdpi https://doi.org/10.3390/antiox12071389 2023-08-01T10:45:43Z High-quality eggs are essential for the sustainability of commercial aquaculture production. Melatonin is a potent candidate for regulating the growth and maturation of oocytes. Therefore, research on the effect of melatonin on marine fish oocytes in vitro has been conducted. The present study successfully established a culture system of turbot (Scophthalmus maximus) oocytes in vitro and investigated the effect of melatonin on oocyte meiotic maturation, antioxidant capacity, and the expression of apoptosis-related genes. The cultures showed that turbot Scophthalmus maximus late-vitellogenic denuded oocytes, with diameters of 0.5–0.7 mm, had a low spontaneous maturation rate and exhibited a sensitive response to 17α, 20β-dihydroxyprogesterone (DHP) treatment in vitro. Melatonin increased by four times the rate of oocyte germinal vesicle breakdown (GVBD) in a concentration- and time-dependent manner. The mRNA of melatonin receptor 1 (mtnr1) was significantly upregulated in the oocyte and follicle after treatment with melatonin (4.3 × 10−9 M) for 24 h in vitro, whereas melatonin receptor 2 (mtnr2) and melatonin receptor 3 (mtnr3) remained unchanged. In addition, melatonin significantly increased the activities of catalase, glutathione peroxidase, and superoxide dismutase, as well as the levels of glutathione, while decreasing the levels of malondialdehyde and reactive oxygen species (ROS) levels in turbot oocytes and follicles cultures in vitro. p53, caspase3, and bax mRNAs were significantly downregulated in oocytes and follicles, whereas bcl2 mRNAs were significantly upregulated. In conclusion, the use of turbot late-vitellogenesis oocytes (0.5–0.7 mm) is suitable for establishing a culture system in vitro. Melatonin promotes oocyte meiotic maturation and antioxidative capacity and inhibits apoptosis via the p53-bax-bcl2 and caspase-dependent pathways, which have important potential to improve the maturation and quality of oocytes. Text Scophthalmus maximus Turbot MDPI Open Access Publishing Antioxidants 12 7 1389 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Scophthalmus maximus oocyte culture and maturation melatonin antioxidative capacity apoptosis |
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Scophthalmus maximus oocyte culture and maturation melatonin antioxidative capacity apoptosis Jiarong Zhang Feixia Li Xiaoyu Zhang Ting Xie Hongyu Qin Junxian Lv Yunhong Gao Mingyue Li Yuntao Gao Yudong Jia Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro |
topic_facet |
Scophthalmus maximus oocyte culture and maturation melatonin antioxidative capacity apoptosis |
description |
High-quality eggs are essential for the sustainability of commercial aquaculture production. Melatonin is a potent candidate for regulating the growth and maturation of oocytes. Therefore, research on the effect of melatonin on marine fish oocytes in vitro has been conducted. The present study successfully established a culture system of turbot (Scophthalmus maximus) oocytes in vitro and investigated the effect of melatonin on oocyte meiotic maturation, antioxidant capacity, and the expression of apoptosis-related genes. The cultures showed that turbot Scophthalmus maximus late-vitellogenic denuded oocytes, with diameters of 0.5–0.7 mm, had a low spontaneous maturation rate and exhibited a sensitive response to 17α, 20β-dihydroxyprogesterone (DHP) treatment in vitro. Melatonin increased by four times the rate of oocyte germinal vesicle breakdown (GVBD) in a concentration- and time-dependent manner. The mRNA of melatonin receptor 1 (mtnr1) was significantly upregulated in the oocyte and follicle after treatment with melatonin (4.3 × 10−9 M) for 24 h in vitro, whereas melatonin receptor 2 (mtnr2) and melatonin receptor 3 (mtnr3) remained unchanged. In addition, melatonin significantly increased the activities of catalase, glutathione peroxidase, and superoxide dismutase, as well as the levels of glutathione, while decreasing the levels of malondialdehyde and reactive oxygen species (ROS) levels in turbot oocytes and follicles cultures in vitro. p53, caspase3, and bax mRNAs were significantly downregulated in oocytes and follicles, whereas bcl2 mRNAs were significantly upregulated. In conclusion, the use of turbot late-vitellogenesis oocytes (0.5–0.7 mm) is suitable for establishing a culture system in vitro. Melatonin promotes oocyte meiotic maturation and antioxidative capacity and inhibits apoptosis via the p53-bax-bcl2 and caspase-dependent pathways, which have important potential to improve the maturation and quality of oocytes. |
format |
Text |
author |
Jiarong Zhang Feixia Li Xiaoyu Zhang Ting Xie Hongyu Qin Junxian Lv Yunhong Gao Mingyue Li Yuntao Gao Yudong Jia |
author_facet |
Jiarong Zhang Feixia Li Xiaoyu Zhang Ting Xie Hongyu Qin Junxian Lv Yunhong Gao Mingyue Li Yuntao Gao Yudong Jia |
author_sort |
Jiarong Zhang |
title |
Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro |
title_short |
Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro |
title_full |
Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro |
title_fullStr |
Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro |
title_full_unstemmed |
Melatonin Improves Turbot Oocyte Meiotic Maturation and Antioxidant Capacity, Inhibits Apoptosis-Related Genes mRNAs In Vitro |
title_sort |
melatonin improves turbot oocyte meiotic maturation and antioxidant capacity, inhibits apoptosis-related genes mrnas in vitro |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/antiox12071389 |
op_coverage |
agris |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Antioxidants; Volume 12; Issue 7; Pages: 1389 |
op_relation |
Health Outcomes of Antioxidants and Oxidative Stress https://dx.doi.org/10.3390/antiox12071389 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox12071389 |
container_title |
Antioxidants |
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12 |
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7 |
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1389 |
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