Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing
Peptide self-assembling materials have received significant attention from researchers in recent years, emerging as a popular field in biological, environmental, medical, and other new materials studies. In this study, we utilized controllable enzymatic hydrolysis technology (animal proteases) to ob...
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ftmdpi:oai:mdpi.com:/2076-3921/12/6/1190/ 2023-08-20T04:06:04+02:00 Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing Dingyi Yu Shenghao Cui Liqi Chen Shuang Zheng Di Zhao Xinyu Yin Faming Yang Jingdi Chen agris 2023-05-31 application/pdf https://doi.org/10.3390/antiox12061190 EN eng Multidisciplinary Digital Publishing Institute Natural and Synthetic Antioxidants https://dx.doi.org/10.3390/antiox12061190 https://creativecommons.org/licenses/by/4.0/ Antioxidants; Volume 12; Issue 6; Pages: 1190 marine-derived bioactive peptides self-assembled peptides materials wound healing topical application molecular docking Text 2023 ftmdpi https://doi.org/10.3390/antiox12061190 2023-08-01T10:17:54Z Peptide self-assembling materials have received significant attention from researchers in recent years, emerging as a popular field in biological, environmental, medical, and other new materials studies. In this study, we utilized controllable enzymatic hydrolysis technology (animal proteases) to obtain supramolecular peptide self-assembling materials (CAPs) from the Pacific oyster (Crassostrea gigas). We conducted physicochemical analyses to explore the pro-healing mechanisms of CAPs on skin wounds in both in vitro and in vivo experiments through a topical application. The results demonstrated that CAPs exhibit a pH-responsive behavior for self-assembly and consist of peptides ranging from 550 to 2300 Da in molecular weight, with peptide chain lengths of mainly 11–16 amino acids. In vitro experiments indicated that CAPs display a procoagulant effect, free radical scavenging activity, and promote the proliferation of HaCaTs (112.74% and 127.61%). Moreover, our in vivo experiments demonstrated that CAPs possess the ability to mitigate inflammation, boost fibroblast proliferation, and promote revascularization, which accelerates the epithelialization process. Consequently, a balanced collagen I/III ratio in the repaired tissue and the promotion of hair follicle regeneration were observed. With these remarkable findings, CAPs can be regarded as a natural and secure treatment option with high efficacy for skin wound healing. The potential of CAPs to be further developed for traceless skin wound healing is an exciting area for future research and development. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific Antioxidants 12 6 1190 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
marine-derived bioactive peptides self-assembled peptides materials wound healing topical application molecular docking |
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marine-derived bioactive peptides self-assembled peptides materials wound healing topical application molecular docking Dingyi Yu Shenghao Cui Liqi Chen Shuang Zheng Di Zhao Xinyu Yin Faming Yang Jingdi Chen Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing |
topic_facet |
marine-derived bioactive peptides self-assembled peptides materials wound healing topical application molecular docking |
description |
Peptide self-assembling materials have received significant attention from researchers in recent years, emerging as a popular field in biological, environmental, medical, and other new materials studies. In this study, we utilized controllable enzymatic hydrolysis technology (animal proteases) to obtain supramolecular peptide self-assembling materials (CAPs) from the Pacific oyster (Crassostrea gigas). We conducted physicochemical analyses to explore the pro-healing mechanisms of CAPs on skin wounds in both in vitro and in vivo experiments through a topical application. The results demonstrated that CAPs exhibit a pH-responsive behavior for self-assembly and consist of peptides ranging from 550 to 2300 Da in molecular weight, with peptide chain lengths of mainly 11–16 amino acids. In vitro experiments indicated that CAPs display a procoagulant effect, free radical scavenging activity, and promote the proliferation of HaCaTs (112.74% and 127.61%). Moreover, our in vivo experiments demonstrated that CAPs possess the ability to mitigate inflammation, boost fibroblast proliferation, and promote revascularization, which accelerates the epithelialization process. Consequently, a balanced collagen I/III ratio in the repaired tissue and the promotion of hair follicle regeneration were observed. With these remarkable findings, CAPs can be regarded as a natural and secure treatment option with high efficacy for skin wound healing. The potential of CAPs to be further developed for traceless skin wound healing is an exciting area for future research and development. |
format |
Text |
author |
Dingyi Yu Shenghao Cui Liqi Chen Shuang Zheng Di Zhao Xinyu Yin Faming Yang Jingdi Chen |
author_facet |
Dingyi Yu Shenghao Cui Liqi Chen Shuang Zheng Di Zhao Xinyu Yin Faming Yang Jingdi Chen |
author_sort |
Dingyi Yu |
title |
Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing |
title_short |
Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing |
title_full |
Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing |
title_fullStr |
Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing |
title_full_unstemmed |
Marine-Derived Bioactive Peptides Self-Assembled Multifunctional Materials: Antioxidant and Wound Healing |
title_sort |
marine-derived bioactive peptides self-assembled multifunctional materials: antioxidant and wound healing |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/antiox12061190 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Antioxidants; Volume 12; Issue 6; Pages: 1190 |
op_relation |
Natural and Synthetic Antioxidants https://dx.doi.org/10.3390/antiox12061190 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/antiox12061190 |
container_title |
Antioxidants |
container_volume |
12 |
container_issue |
6 |
container_start_page |
1190 |
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1774716988607692800 |